Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	624,906	A→C	27.1%	intergenic (‑164/+64)	potE ← / ← adh_1	Putrescine transporter PotE/Aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	624,906	0	A	C	27.1%	54.8 / 3.5	44	intergenic (‑164/+64)	potE/adh_1	Putrescine transporter PotE/Aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base C (7/6);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAGGGTTCCGGGCCGATGGTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCA  >  ACB122/624772‑625040                                                                                                                                       |                                                                                                                                       ggAAGGGTTCCGGGCCGATGGTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCAc                                                                                                                                       >  7:396161/1‑136 (MQ=255)      ggTTCCGGGCCGAGGGTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGc                                                                                                                                   <  2:194453/135‑1 (MQ=255)           cGGGCCGATGGTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGa                                                                                                                             <  7:296092/136‑1 (MQ=255)                   tggggctGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGGTACCGCCCTGGGGTTTGTGTAAAGCAAGGCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACgg                                                                                                                     <  8:35096/130‑1 (MQ=39)                    ggTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGGTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTCCCGCCCCGGCTCTGGAACCAACgg                                                                                                                     <  6:155028/135‑1 (MQ=255)                           ggTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACACGTACCGCACCGGCTCTGGAACCAACGGTGAACCt                                                                                                              >  3:301848/1‑135 (MQ=255)                               ctgcAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAg                                                                                                          <  4:185700/135‑1 (MQ=255)                                tgcAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACtgtg                                                                                                                   <  4:239186/125‑1 (MQ=255)                                tgcAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACtgtg                                                                                                                   >  3:239186/1‑125 (MQ=255)                                tgcAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTAGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGTACCAACGGTGAACCTGTa                                                                                                           >  4:366070/1‑133 (MQ=255)                                   aGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCCCAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGc                                                                                                      >  4:307590/1‑135 (MQ=255)                                    ggacccGAGCCAGAGCGGTTGGGGCACGGGTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCGCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCCCTGGAACCAACGGTGAACCTGTAGGAGCgg                                                                                                    <  7:364636/131‑1 (MQ=39)                                          gAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCCCACGTACCGCCCCGGCTCTGGAACCAACGGTGAACCGGTAGGCGCGGGTTTa                                                                                               >  3:323127/1‑135 (MQ=255)                                          gAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACACGTAACCCCCCCGCCCTGGACCCAACGGTGGACCGGTAGGAGCGGGTTTa                                                                                               >  4:224541/1‑135 (MQ=38)                                          gAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCGCCCCCAGGTACCGCCCCGGCGCTGGAACCAACCGTGTACCTGTAGGCGCGGGTTTa                                                                                               >  1:268250/1‑135 (MQ=255)                                          gAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGGTTTGTGTAAAGCAAGTCCGGCCTCTTGGCGGGGTGGCCCCCACCCCCCGGTACCCCCCCCGCGCTGGGACCCCACGTGCACCTGAAGGACCGGGgttt                                                                                               >  1:284659/1‑134 (MQ=255)                                            gCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCGTGGCCGCGCCCCCCGGTCCCGCCCCGGGGGGGGGACCCACGGGGGACCGGGAGGGGCGGGTTTTcc                                                                                             >  1:340417/1‑135 (MQ=255)                                                         gggCACGGTTACCGCCCTGGGTTTGGTGTAAAGCAAGCCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGAACCGCACCGGCTCTGGAACCACCGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCg                                                                                <  3:307590/133‑1 (MQ=39)                                                         gggCACGGTACCCGCCCTGGGTTTTGTGTAAAGCCAGTCCGGCCTCTGCGCGGGCTGGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGAAGGAGCGGGTTTACCCGCGAAGAAGGCg                                                                                <  8:124761/133‑1 (MQ=255)                                                         gTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGGGTGCCCGCGCCCCCAGGTTCCCCACCGGCTCTGGCACCAACCGTGGACCGGTAGGAGCGGGGTTTCCCGGGAAGAAGGCg                                                                                >  3:40956/1‑135 (MQ=255)                                                         gTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTAGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAAACAACGGTGCACCTGTATGAGCGGGGTTACCCGCGAAGAAGGCg                                                                                >  6:311430/1‑135 (MQ=39)                                                         gTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGGCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCg                                                                                <  3:95808/135‑1 (MQ=255)                                                         gTGCACGGCTACCGCCCTGGGTTTTGTGTAACGCAAGGCCGGCGTCTTCGCGGGCTTGCCCGCTCCCACAGGTCCCGCACCGGCGGGGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCg                                                                                <  1:249017/135‑1 (MQ=38)                                                                   aCCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTTCCCCCTCCCCCAGGTGCCCCACCGGGGCTGGGACCAACCGGGCACCGGTAGGAGCGGGTTTACCCGCGCAGAAGGcgccgccgg                                                                         >  1:308004/1‑129 (MQ=255)                                                                              ttttGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCACCCCCAGGTAACCCCCCCGCACTGGAACCAACGGTGAACCTGTATGCGCGGGGTTACCCGCGCAGCAGGCGCCGCGGTGTAGAAGAAGccac                                                           >  2:321971/1‑133 (MQ=255)                                                                                  gtgtAAAGCAAGTCCGGCCTCTGCGCGGGCTTGCCCGCTCCCACAGGTACCGCCCCGGCTCTGGAACCAACGGTGAACCTGTAGTAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCgg                                                       <  7:96558/135‑1 (MQ=255)                                                                                           aaGTCCGGCCTCTTCGTGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCg                                             >  8:131883/1‑136 (MQ=255)                                                                                                     tctTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGCACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCGAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCa                                      >  1:54885/1‑133 (MQ=255)                                                                                                     tctTCGCGGGCTGGCCCGCTCCCACCGGTACCCCCCCGGATCTGGAACAAAGGGTTAACCTGTTGGCGCGGGTTTACCCGGTAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCAcc                                    <  3:224541/135‑1 (MQ=21)                                                                                                        tCGCGGGCTTGCCCGCTCCCACAGTTACCGCACCGGCTCTGCAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACca                                   <  3:366070/133‑1 (MQ=255)                                                                                                               cTTGCCCGCTCCCCCAGGTACCGCCCCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCtt                         <  7:291700/136‑1 (MQ=255)                                                                                                               cTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCtt                         <  7:292494/136‑1 (MQ=255)                                                                                                                ttGCCCGCACCCACAGGTTACGCCCCGGCTCGGGAACCAACGGTGAACCAGTAGGAGCCGGTTTACCCGCGAAGAATCCGACGCGGGCTAGAAGAAGCCCAGCGGATTGATGTAGTAGCTCACCCGCAGGGTCtt                         >  2:136356/1‑135 (MQ=18)                                                                                                                    ccGCGCCCACAGGTCCGGCCCCGGCTCGGTACCCAACGGTGAACCTGTTGGAGCGGGGTTACCCGCGATGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTct                    <  8:83818/136‑1 (MQ=17)                                                                                                                      gCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCgg                                                                                                    <  6:197422/54‑1 (MQ=255)                                                                                                                      gCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCgg                                                                                                    >  5:197422/1‑54 (MQ=255)                                                                                                                           cacaGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGa               <  4:301848/134‑1 (MQ=255)                                                                                                                           cacaGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGa               >  8:238351/1‑134 (MQ=255)                                                                                                                               ggccccGCCCCGGCCCTGCAACCACCGGTTAACCGGTCGGCGGGGGTTACCCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTg          <  5:397128/131‑1 (MQ=17)                                                                                                                                   cggCAGCGGCTGTTGAACCAAGGTTGAACTTTTCGGCGGGGGTTTACCCGCGAAGATGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATcc      <  6:58854/133‑1 (MQ=17)                                                                                                                                   ccGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGGGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCa     <  8:44968/136‑1 (MQ=255)                                                                                                                                     gCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCa     >  3:368601/1‑134 (MQ=255)                                                                                                                                     gCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCa     >  1:11604/1‑134 (MQ=255)                                                                                                                                     gCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGCCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCa     >  5:70095/1‑134 (MQ=255)                                                                                                                                       aCCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGca  >  1:4937/1‑135 (MQ=255)                                                                                                                                       aCCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCAGATAGTGATCCAGca  >  4:289125/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                       GGAAGGGTTCCGGGCCGATGGTGATGGGTGCTGCAGGAAGCGAGCCAGAGCGGTTGGTGCACGGTTACCGCCCTGGGTTTTGTGTAAAGCAAGTCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTACCGCACCGGCTCTGGAACCAACGGTGAACCTGTAGGAGCGGGTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCA  >  ACB122/624772‑625040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑