Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 999,352 G→A 8.0% R411Q (CGG→CAG)  fecA_1 → Fe(3 ) dicitrate transport protein FecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122999,3520GA 8.0% 135.4 / 3.8 50R411Q (CGG→CAG) fecA_1Fe(3 ) dicitrate transport protein FecA
Reads supporting (aligned to +/- strand):  ref base G (19/27);  new base A (2/2);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GCGATTACGACTACTTCGCGGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGGTTACCGTTACCTGAAGGAAGCGA  >  ACB122/999220‑999486
                                                                                                                                    |                                                                                                                                      
gcgATTACGACTACTTCGCGGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCggg                                                                                                                                      <  3:293988/135‑1 (MQ=255)
 cgATTACGACTACTTCGCGGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCggg                                                                                                                                      >  3:158508/1‑134 (MQ=255)
        gACTACTTCGCGGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAg                                                                                                                              <  3:207125/135‑1 (MQ=255)
                 gcgGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTgtcgt                                                                                                                    >  7:161208/1‑136 (MQ=255)
                     gCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTcgtcgt                                                                                                                 <  4:361202/135‑1 (MQ=255)
                       cGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTAc                                                                                                               >  3:17026/1‑135 (MQ=255)
                          cgcAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCcg                                                                                                            <  1:301959/135‑1 (MQ=255)
                          cgcAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCcg                                                                                                            <  6:198761/135‑1 (MQ=255)
                          cgcAAGGATGTGGCGTTCAAATGGCAGCGGCAGCGCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTCCACCGACCGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCcg                                                                                                            <  4:15085/135‑1 (MQ=255)
                           gcAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCCCCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACCAAAGCCTCGCGGGACATGACGACGTTGTCGTCGTCCCcgcg                                                                                                          >  7:173542/1‑136 (MQ=255)
                            cAAGGAGGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGGCGTCGTACCcgcg                                                                                                          <  6:170072/135‑1 (MQ=255)
                                 aTGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACt                                                                                                     <  2:268074/135‑1 (MQ=255)
                                       cGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACcaca                                                                                               >  1:158065/1‑135 (MQ=255)
                                       cGTTCAAATGGCAGCGGCAGCGCGACGATTCCACCCAGTTCGAAGTGCTGACCTACTACCCCGACAGCTTCCGAGGCAGCCCAATAGCCTCGCGGGACATGAAGCCGTTGTCGTCGTACCCGCGCAACTACcaca                                                                                               >  5:9788/1‑135 (MQ=255)
                                         ttCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCacac                                                                                              <  5:177950/134‑1 (MQ=255)
                                          tCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGCCCTCCTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGGTGTCGTCGTACCCGCGCAACTACCACACCt                                                                                            <  5:144715/135‑1 (MQ=255)
                                          tCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCt                                                                                            <  4:158508/135‑1 (MQ=255)
                                          tCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCt                                                                                            <  3:332047/135‑1 (MQ=255)
                                            aaaTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCg                                                                                         >  8:375307/1‑136 (MQ=255)
                                            aaaTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCAGGACATGAAGACg                                                                                                                           >  1:148492/1‑102 (MQ=255)
                                            aaaTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCAGGACATGAAGACg                                                                                                                           >  1:153493/1‑102 (MQ=255)
                                            aaaTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCAGGACATGAAGACg                                                                                                                           <  2:153493/102‑1 (MQ=255)
                                            aaaTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCAGGACATGAAGACg                                                                                                                           <  2:148492/102‑1 (MQ=255)
                                              aTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCCGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCCCCCCTACGc                                                                                        >  1:375913/1‑135 (MQ=255)
                                              aTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGAc                                                                                                                            <  6:344886/99‑1 (MQ=255)
                                              aTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGAc                                                                                                                            >  5:344886/1‑99 (MQ=255)
                                              aTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTCCCACACCTTCg                                                                                         >  3:37008/1‑134 (MQ=255)
                                              aTGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCg                                                                                         >  2:143768/1‑134 (MQ=255)
                                                   agcggcagcTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTg                                                                                   <  1:143768/135‑1 (MQ=255)
                                                                cgaTGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTcg                                                                     <  7:237589/136‑1 (MQ=255)
                                                                   tGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCGTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTcgcg                                                                   <  6:241100/135‑1 (MQ=255)
                                                                                gAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCg                                                     >  8:267855/1‑136 (MQ=255)
                                                                                   gTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGc                                                   <  5:278513/135‑1 (MQ=255)
                                                                                    tGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGccc                                                 <  7:375307/136‑1 (MQ=255)
                                                                                     gCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGccc                                                 <  3:388841/135‑1 (MQ=255)
                                                                                     gCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGccc                                                 <  3:382331/135‑1 (MQ=255)
                                                                                       tGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCaa                                               <  4:17026/135‑1 (MQ=255)
                                                                                          ccTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGcccaccca                                            >  2:207828/1‑135 (MQ=255)
                                                                                          ccTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAacca                                            >  3:97065/1‑135 (MQ=255)
                                                                                                   ccGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAg                                   <  2:327689/135‑1 (MQ=255)
                                                                                                   ccGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATCAAGACGTTGTCGTCGTCCCCGCGCAACTACCACACCTTCGCCATTGACCCCCGTGTGTCGCGCATCGTCTTTGCCGGCCCTACCACCCACGAAGt                                  >  8:392490/1‑136 (MQ=255)
                                                                                                     gtcAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTc                                 <  6:54922/133‑1 (MQ=255)
                                                                                                     gACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTc                                 <  6:386658/135‑1 (MQ=255)
                                                                                                       cAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCATCTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAgt                              >  7:259754/1‑136 (MQ=255)
                                                                                                       cAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAgt                              >  8:27803/1‑136 (MQ=255)
                                                                                                        aGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAgtg                             >  8:118242/1‑136 (MQ=255)
                                                                                                         gCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAgtg                             >  5:99012/1‑135 (MQ=255)
                                                                                                         gCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAgtg                             >  3:348210/1‑135 (MQ=255)
                                                                                                          cTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTc                           <  7:267855/136‑1 (MQ=255)
                                                                                                               gcgTCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGgtt                       <  4:59964/135‑1 (MQ=255)
                                                                                                               gaggCAGCACAATAGCCTCGCGTGAAATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGgtt                       <  6:9788/133‑1 (MQ=255)
                                                                                                                               cTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGGTTACCGTTACCTgaagga       <  6:251339/135‑1 (MQ=255)
                                                                                                                                   cgGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCCCCCAGGAAGTCAGTGTCGGTTTCCGTTTCCTGAAGGTAGCg   >  2:381565/1‑135 (MQ=255)
                                                                                                                                   cgGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGGTTACCGTTACCTGCAGGAAGCGa  >  8:344590/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCGATTACGACTACTTCGCGGGCCGGCGCAAGGATGTGTCGTTCAAATGGCAGCGGCAGCTCGACGATGCCACCCAGTTCGAAGTGCTGACCTACTACACCGACAGCTTCCGCGGCAGCACAATAGCCTCGCGGGACATGAAGACGTTGTCGTCGTACCCGCGCAACTACCACACCTTCGCCATTGAACCCCGTGTGTCGCGCATCTTCTTTGCCGGCCCAACCACCCAGGAAGTCAGTGTCGGTTACCGTTACCTGAAGGAAGCGA  >  ACB122/999220‑999486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑