Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,125,327	C→A	6.1%	L13L (CTG→CTT)	CJ019_01064 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,125,327	0	C	A	6.1%	201.7 / 4.1	67	L13L (CTG→CTT)	CJ019_01064	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base A (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

AGACCGGGTGCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCCGGCGGGGACGTTT  >  ACB122/1125194‑1125457                                                                                                                                      |                                                                                                                                   agaCCGGGTGCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGATCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAg                                                                                                                                  <  7:328954/136‑1 (MQ=255)     cGGGTGCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTg                                                                                                                              >  8:340492/1‑136 (MQ=255)        gTGCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGAc                                                                                                                            >  1:7326/1‑135 (MQ=255)          gCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTc                                                                                                                          >  3:202269/1‑135 (MQ=255)          gCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACAAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTcc                                                                                                                         >  8:120656/1‑136 (MQ=255)             gCTCTGCGATTCGGGTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCgg                                                                                                                       <  6:5494/135‑1 (MQ=255)                      ttCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTc                                                                                                              <  5:94795/135‑1 (MQ=255)                         ggTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTg                                                                                                           <  1:392338/135‑1 (MQ=255)                           ttCCGCCAGGCCAGGCGCGGGCCACCGACCAGGTAGTTCTCCGGGGGAACTTCACACCAGAGCCCTGAGAGCTGTCACGCCAGCGCCTGGTCGGAGTGCTCGGACGGCCTGCTGACGCCGGCGCCCATCCGTGCg                                                                                                         >  4:204038/1‑135 (MQ=255)                           ttCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCg                                                                                                         <  6:336270/135‑1 (MQ=255)                           ttCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGt                                                                                                        >  8:86581/1‑136 (MQ=255)                           ttCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGt                                                                                                        <  7:86581/136‑1 (MQ=255)                           ttCAGCCAAGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCAGTCGTGCGt                                                                                                        >  8:120506/1‑136 (MQ=255)                                     cAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCa                                                                                               <  2:251455/135‑1 (MQ=255)                                               gCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGTCCTTTAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGttt                                                                                     <  4:297603/135‑1 (MQ=255)                                               gCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGttt                                                                                     <  6:348491/135‑1 (MQ=255)                                                   ccGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCt                                                                                <  7:280949/136‑1 (MQ=255)                                                      aCCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGAt                                                                             <  8:297333/136‑1 (MQ=255)                                                       ccATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCTCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGAt                                                                             <  4:292626/135‑1 (MQ=255)                                                         aTGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCa                                                                           <  3:95150/135‑1 (MQ=255)                                                         aTGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCa                                                                           <  5:86937/135‑1 (MQ=255)                                                           gTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCCTTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCTAg                                                                         >  5:13469/1‑135 (MQ=255)                                                            tAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGa                                                                        >  3:358372/1‑135 (MQ=255)                                                            tAGTTCTACGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGATGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGa                                                                        >  6:187438/1‑135 (MQ=255)                                                             aGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCGTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATGAAGAc                                                                       >  1:237052/1‑135 (MQ=255)                                                              gTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCGGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     <  7:114868/136‑1 (MQ=255)                                                              gTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     <  8:389292/136‑1 (MQ=255)                                                              gTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACt                                                                      <  7:156139/135‑1 (MQ=255)                                                               ttCTCCGGGTGAACTTCCAACCTTCGCCCTTAGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCGGAGTCCGGCGCCGATTCGTGCGTCTGTGTTCATGGTCGGTTTCCACTGATCAAGACtt                                                                     <  6:108823/135‑1 (MQ=255)                                                               ttCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     <  3:351547/135‑1 (MQ=255)                                                               ttCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     <  2:251710/135‑1 (MQ=255)                                                                    cGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTc                                                                                          <  2:91052/109‑1 (MQ=255)                                                                    cGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTc                                                                                          >  1:91052/1‑109 (MQ=255)                                                                     gggTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGgt                                                              >  7:71023/1‑136 (MQ=255)                                                                     gggTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGgt                                                              >  7:21886/1‑136 (MQ=255)                                                                         gAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGgtg                                                             >  5:45747/1‑133 (MQ=255)                                                                            cTTCAAACCAGCGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCcgcg                                                       >  7:217348/1‑136 (MQ=255)                                                                                    cAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTGGTAGTCCTCGGCCTTCACGCTGACTCCGTCGCCCATTCGTGCGTCTGTGTTCATCGTCGGTTACGCCTGATCAAGACTTGATAGCACTCAGAGAGCCAAt                                                >  3:81055/1‑135 (MQ=255)                                                                                        gcacTTCGAGAGTGCACGCCAGCGGCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGGCTGTGTTCATGGTCGGTTTCCCCTGATCAATACTTGATAGGTGTCAGCGAGCCCATGCcgg                                           <  8:268107/133‑1 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCcggcg                                         >  7:325598/1‑136 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCcggc                                          >  4:373567/1‑135 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTAAGGCTGACTCCGGCGCCCATTCGTGCg                                                                                                         >  6:203058/1‑72 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTAAGGCTGACTCCGGCGCCCATTCGTGCg                                                                                                         >  6:196858/1‑72 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTAAGGCTGACTCCGGCGCCCATTCGTGCg                                                                                                         <  5:196858/72‑1 (MQ=255)                                                                                          ccTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTAAGGCTGACTCCGGCGCCCATTCGTGCg                                                                                                         <  5:203058/72‑1 (MQ=255)                                                                                               gagaTTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     <  2:275472/103‑1 (MQ=255)                                                                                               gagaTTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACtt                                                                     >  1:275472/1‑103 (MQ=255)                                                                                               gagaTTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGgtg                                     >  4:95105/1‑135 (MQ=255)                                                                                                 gaTTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTAATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTc                                   >  2:80193/1‑135 (MQ=255)                                                                                                  aTTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCtt                                 <  7:120656/136‑1 (MQ=255)                                                                                                   ttGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTCGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCtt                                 <  2:19913/135‑1 (MQ=255)                                                                                                         gCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGAt                                                                             <  7:240018/85‑1 (MQ=255)                                                                                                         gCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGAt                                                                             >  8:240018/1‑85 (MQ=255)                                                                                                         gCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCAGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGa                           >  4:200154/1‑135 (MQ=255)                                                                                                          ccAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGAtg                         <  8:71023/136‑1 (MQ=255)                                                                                                           cAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGAtg                         <  6:290875/135‑1 (MQ=255)                                                                                                              cgcCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCTGCGAGCCCCTGCCGGCGCGAGTCGGGATCGAtgt                        >  3:314484/1‑133 (MQ=255)                                                                                                                      cGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCAATCGAGCGTCTGTGTTCATGGTCTGATACCCCAGATCAAGACTTGATAGGTGTCAGCGACCCCAAGCCGGCGGGTGTCTTGCTCGATGTGCAGGTGCcg              >  5:371582/1‑135 (MQ=255)                                                                                                                        tAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCcggcg           >  7:369275/1‑136 (MQ=255)                                                                                                                                ggCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCtt                                 >  7:45144/1‑106 (MQ=255)                                                                                                                                ggCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCtt                                 <  8:45144/106‑1 (MQ=255)                                                                                                                                ggCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGACCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCCGGCGGGGACGtt   >  8:364565/1‑136 (MQ=255)                                                                                                                                 gCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCCGGCGGGGACGttt  <  8:65176/136‑1 (MQ=255)                                                                                                                                  ccTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCCGGCGGGGACGttt  >  6:143537/1‑135 (MQ=255)                                                                                                                                  ccTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGAGCCGGCGGGGACGttt  >  6:180742/1‑135 (MQ=255)                                                                                                                                     tCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCcggcg                                         >  8:352295/1‑93 (MQ=255)                                                                                                                                     tCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCcggcg                                         <  7:352295/93‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   AGACCGGGTGCCGCTCTGCGATTCGGTTCAGCCAGGCAAGGCGAGGGCCACCGACCATGTAGTTCTCCGGGTGAACTTCAAACCAGAGCCCTTCGAGATTGCACGCCAGCGCCTGGTCGTAGTGCTCGGCCTTCAGGCTGACTCCGGCGCCCATTCGTGCGTCTGTGTTCATGGTCGGTTTCCCCTGATCAAGACTTGATAGGTGTCAGCGAGCCCATGCCGGCGGGTGTCTTGATCGATGTGCAGGTGCCGGCGGGGACGTTT  >  ACB122/1125194‑1125457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑