| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,188,694 | T→G | 46.3% | L43R (CTG→CGG) | CJ019_01117 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,188,694 | 0 | T | G | 46.3% | 1.4 / 2.1 | 21 | L43R (CTG→CGG) | CJ019_01117 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/7); new base G (4/6); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTGGTGCT > ACB122/1188564‑1188820 | gTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGCCCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACCCCCGGCCACGGCGCCTGCGGTGGCCGACCGCCCGCTGccc > 1:61365/1‑135 (MQ=255)gTGATGCTCACCTCGCGTCTGCTCACCGGAGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCCGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCCCGGCGCCTGCGGTGGCCGACACCCCGCTGccc > 4:67777/1‑135 (MQ=255) gATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGATAGCCCGCTGCCCggg > 7:176433/1‑136 (MQ=255) gATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGAGCGCCCCCGGCGCgg > 2:392787/1‑135 (MQ=255) gATGCGCACCTCGCGGCTGCTCACCGGGGCTGGCCTGCCCCTGGCCGGGTGGCTTTCTGCGCAGGGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACAGCCCGCTGCCCgg < 2:69823/135‑1 (MQ=255) cACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACCGCCCGCGGGCCCGGCTGAt > 6:320079/1‑135 (MQ=255) cTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACCGCCCGCGGCCCGGGCGGAtggttggggac > 4:173846/1‑131 (MQ=255) tGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACAGCCCGCGGCCCGGGCCGCTGGGGGGGGCCTTGCAGGGGCCGccc > 8:366563/1‑136 (MQ=255) cTGGCGGGCGGGCTTGCTGCGATGTGTGGGCTGCAGTTCACCGGCCTCCCACACCGGGCCACGGCCCCGGCGGGGGCCGACAGCCCGCGGCCCGGGCTGATGGTGGGTCACTGGCAGGCGCCGCCCGACGACGgc < 4:292083/135‑1 (MQ=255) cGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCAGACAGCCCCCTTCCCGGGGGGATGGGTTGTGACAGGCCGGCGCCGCCCCacgacgaccgcc > 2:53774/1‑128 (MQ=255) ggccAGGGTGTGCGGCAGTTGCCCCGGCAGCCACACCGGGCCACGGCGCCGGGGGGGGCCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTc < 7:322704/133‑1 (MQ=255) cgatgtgtttGCGGCAGTGGCGCCGGCACCAACACCCGCCAAGGGCGCGTGGGGGGCCCGACAGCCCGCTGCCCGGGCTTATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCt < 7:316812/132‑1 (MQ=255) ggcccgCCCCACCCGGCCCGGGGGCGGGCGGGGGCCGACCGCCCGCTGCCCGGGCTGATGGTTGGGCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGcc < 8:337835/131‑1 (MQ=255) gcccgCCACCCCGGGCCCGGGCGCGGGGGGGGGCCCCCAGCCCGCGGCCCGGGCTGTTGGTGGGGCACGGCCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCt < 7:100600/132‑1 (MQ=255) ccgCCACCCCCGGCCCGGGCGCGGCCGGGGCCCGACAGCCCGGTGCCGGGGCTGATGGTTGGACAGTGCCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCa < 8:121246/134‑1 (MQ=255) cgcCTGGGGTGGCCTACAGCCCGCTGCCGGGGATGAGGGTTGGTCACTGGCTGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGgccgc < 8:320810/136‑1 (MQ=255) ggggTGGCCCACAGCCCGAGGCCGGGGCTGTTGGGTGGTCTCTGGCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAgtg < 8:187010/134‑1 (MQ=255) gCCAACCGCCGGCGGCCGGGGGTGTTGGTGGGCCAGTGCCCGGGGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 1:217709/135‑1 (MQ=255) ccgcaCAGCCGGGGGCCGGGGCTGTTGGTGGGTCACTGGCTGGCGCGGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 3:73397/131‑1 (MQ=255) cccccgCCCGCGGCCGGGGCTTTGGGTGGGCCACGGGCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtggt < 1:285431/130‑1 (MQ=255) ccgCCCGCTGCCCGGGCTGATGTTTGGTCATTGGCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTGGtgct < 4:151123/133‑1 (MQ=255) | GTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGGCCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTGGTGCT > ACB122/1188564‑1188820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |