Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,196,474 T→G 17.3% G55G (GGT→GGG)  CJ019_01126 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,196,4740TG17.3% 97.0 / 2.6 59G55G (GGT→GGG) CJ019_01126hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/34);  new base G (10/2);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 6.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGCAG  >  ACB122/1196348‑1196606
                                                                                                                              |                                                                                                                                    
cGCCTGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCt                                                                                                                              <  8:3166/135‑1 (MQ=255)
cGCCTGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCt                                                                                                                              <  7:376020/135‑1 (MQ=255)
   cTGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACCGGCGGCCAGCCTCCAAGGCGGGCCCGGGTTGAACCTGGa                                                                                                                           >  4:195241/1‑135 (MQ=255)
    tGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGAGAAGGCATTGCGCAAGGGCGCGTGCTTGGCCAGTGGCGTGTGCGGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAg                                                                                                                          <  1:300562/135‑1 (MQ=255)
         aCTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGATCCAGGAGCGGCTa                                                                                                                    >  7:71281/1‑136 (MQ=255)
             aTCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  8:330849/136‑1 (MQ=255)
             aTCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  7:279179/136‑1 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGCCCCGGCGGCCCGCCTCCCAGGCGGGGCCGGGTTGGTCCGGGAGCGGCGACggc                                                                                                                >  4:7113/1‑133 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  3:14504/135‑1 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  6:357866/135‑1 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGGTTGAACCTGGAGCGGCTACGTc                                                                                                                <  6:398944/135‑1 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGGGGTGGGCGGACACGCGGCCCCCCCGCCACGCGGGGCCCGGTTGCAACTGGCGCGGcgccg                                                                                                                  >  4:206857/1‑129 (MQ=255)
              tCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGACAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCTGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  5:303770/135‑1 (MQ=255)
              gcGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTc                                                                                                                <  6:292364/134‑1 (MQ=255)
                gCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGGCGCACAGGCGGCCCGCCGCCAAGGCGGGCCCGGGTTGGACCTGGAGCGGCGACGTCAg                                                                                                              >  2:2021/1‑135 (MQ=255)
                gCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGGCGCACAGGCGGCCCGCCGCCAAGGCGGGCCCGGGTTGCACCGGGAGCGGCGACGGCAg                                                                                                              >  1:133685/1‑135 (MQ=255)
                gCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGGACCTGGAGCGGCTGCGTCAg                                                                                                              >  4:77466/1‑135 (MQ=255)
                gCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGGTCTGGCCGCACCGGCGGGCCGCCGCCAAGGCGGGCCCGGGTTGAAACTGGAGCGGCTACGTCAg                                                                                                              >  2:66077/1‑135 (MQ=255)
                  ccTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGGGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCg                                                                                                           <  7:125637/136‑1 (MQ=255)
                   ctgctgctgGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGGGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCg                                                                                                           <  6:71982/135‑1 (MQ=255)
                                  cTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCTGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCTGCTACGTCAGTCGGCCCAGGCCCGTgg                                                                                             <  1:299049/134‑1 (MQ=255)
                                    ggCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCCGCCACCAACGCGGGCCCGGGTTGAACCTGGCGCGGCGACGGCAGTCGGGCCCGGCCCCGggggg                                                                                          >  4:50756/1‑134 (MQ=255)
                                     gCCCTGCGCGAAGGCATTGGGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGCACAGGCGGCCAGCCTCCAAGGCGGGCCCTGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTc                                                                                         <  8:190603/135‑1 (MQ=255)
                                            gcgAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGCACAGGCGGCCCGCCGCCCAGGCGGGCCCGGGTTGAACCGGGAGCGGCTACGTGAGTCGGCCCAGGCCCGGGGGGTGGCACTggt                                                                                 >  8:24576/1‑134 (MQ=255)
                                                        cgcAATGGCGGGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACTGGCGGCCAGCCTCCAAGGCGGGCCCTGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGAt                                                                       <  5:322546/134‑1 (MQ=255)
                                                               gcgcGTGCTTGGCCAGTGGCGTGGGCGGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACgcaagg                                                               <  1:319118/135‑1 (MQ=255)
                                                                cgcgTGCTTGGCCAGTGGCGTGTGCTGGCCGCACAGGCGGCCCGCCGCCAAGGCGGGCCCGGTGTGAACCTGGAGCGGCGACGTCAGTCGGCCCCGGCCCGGGGGGGCGCACTGATTGGAGGGGATACgcaaggc                                                              >  4:341247/1‑135 (MQ=255)
                                                                cgcgTGCTTGGCCAGTGGCGTGTGCTGGCCGAAGAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCTTGGGGTCGAACTGATTGAAGTCGATACgcaagg                                                               <  1:266571/134‑1 (MQ=255)
                                                                cgcgTGCTTGGCCAGTGGCGTGTGCTGGCCCCACAGGCGGCCCGCCAGCAAGGCGGGCCCGGTTTGAACCTGGTGCGGCTACGTCACTCGGCCCAGGCCCGTGGGGGCGCACGGAGTTAACGCGCTACgcaaggca                                                             >  8:320077/1‑136 (MQ=255)
                                                                cgcgTGCTTGGCCAGTGGCGTGGGCTGGGCGCACACGCGGCCCGCCGCCCAGGCGGGCCCGGGGTGGACCGGGGGCGGCTACCTGAGTCGGCCCAGGCCCGGGGGGGCGGACTGATTGTAGTGGAGACgccagg                                                               >  4:345071/1‑134 (MQ=255)
                                                                                  cGTGTGGTGGCCGCACACGCGGCCAGCCTCCAAGGCGGGCCCCGGTTGAACCTGGAGCGGCTACGTCCGTCGGGCCACGCCCGGGGGGGCGAACAGATAGGATTACATACCCCAGGGAAGGCCTGGGAACTGGGt                                            >  6:355048/1‑135 (MQ=255)
                                                                                  cGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGACCCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGt                                            <  4:118449/135‑1 (MQ=255)
                                                                                        cTGGCCGAACAGGCGGCCAGCCTCCACGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGGGGGGTCTAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAgg                                     <  7:51640/136‑1 (MQ=255)
                                                                                               aaCAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGG‑CGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGAt                             <  7:272291/136‑1 (MQ=255)
                                                                                                   ggcggcCAGCCTCCAAGGCGGGCCCGGTTTGAAGCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAg                          >  8:190117/1‑136 (MQ=255)
                                                                                                    gcggcCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAgc                         <  8:301712/136‑1 (MQ=255)
                                                                                                     cggcCAGCCTCCAAGGCGGGCCCGGTGTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAgc                         <  3:50756/135‑1 (MQ=255)
                                                                                                     cggcCAGCCGCCACGGCGGGCCGGGTTTGACCCTGGAGCGGCGACGGCAGTCGGCCCAGGCCCGTGGGGGCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAgc                         <  1:161538/135‑1 (MQ=255)
                                                                                                     cggcCAGCCGCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAgc                         <  6:145250/135‑1 (MQ=255)
                                                                                                     cggcCAGCCGCCAAGGCGGGCCCGGTGCGAACCTGGCGCGGCTACGGCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAgc                         <  1:399075/135‑1 (MQ=255)
                                                                                                          aGCCTCCCAGGCGGGCCCGGGTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGGGGGCAGGTGGCCGATGAGCGCAtg                    <  3:10611/135‑1 (MQ=255)
                                                                                                            ccgccACGGCGGGCCCGGTTTGAACCTGGAGCGGCTTCGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAACGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCAtgct                  <  5:333310/132‑1 (MQ=255)
                                                                                                                 aaGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAgg            <  8:53369/136‑1 (MQ=255)
                                                                                                                 aaGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAg             <  4:10577/135‑1 (MQ=255)
                                                                                                                      gggCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTgg        <  3:345071/135‑1 (MQ=255)
                                                                                                                      gggCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTgg        <  3:341247/135‑1 (MQ=255)
                                                                                                                        gCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGGGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTg     <  7:317701/136‑1 (MQ=255)
                                                                                                                         cccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTg     <  4:312267/135‑1 (MQ=255)
                                                                                                                         cccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGc    >  7:242071/1‑136 (MQ=255)
                                                                                                                          ccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGCTACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGt      >  6:330690/1‑133 (MQ=255)
                                                                                                                          ccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCAtgct                  >  2:357964/1‑121 (MQ=255)
                                                                                                                          ccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCAtgct                  <  1:357964/121‑1 (MQ=255)
                                                                                                                          ccGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTg     >  7:192068/1‑134 (MQ=255)
                                                                                                                           ctgTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGAAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGCag  <  8:70422/134‑1 (MQ=255)
                                                                                                                           cGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGt      >  6:325856/1‑132 (MQ=255)
                                                                                                                           cGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGt      >  1:130597/1‑132 (MQ=255)
                                                                                                                           cGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGCa   >  3:308467/1‑135 (MQ=255)
                                                                                                                           cGGTTTGAACCTGGAGCGGCTACGTCAGGCGGCCCAGGCCCGTGGGGTCGAACTGATTTAAGGCGCTACGCAAGGCAACGCCTGGCCAATGCGTGGGCAGGTGGCCGATGAGCCCATTCTGCAAGGCTGGGTGcc   >  2:175799/1‑134 (MQ=255)
                                                                                                                            ggTGTGAACCTGGAGCGGCTACGTCAGGCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGCag  <  3:7113/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                    
CGCCTGGGGACTGATCGCCCTGCTGCTGGCCTTCCTGGCCCTGCGCGAAGGCATTGCGCAATGGCGCGTGCTTGGCCAGTGGCGTGTGCTGGCCGAACAGGCGGCCAGCCTCCAAGGCGGGCCCGGTTTGAACCTGGAGCGGCTACGTCAGTCGGCCCAGGCCCGTGGGGTCGAACTGATTGAAGTCGATACGCAAGGCAAGGCCTGGCAACTGCGTGGGCAGGTGGCCGATGAGCGCATGCTGCAAGGCTGGGTGCAG  >  ACB122/1196348‑1196606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑