Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,202,727 G→A 7.8% G95S (GGC→AGC)  proY_1 → Proline‑specific permease ProY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,202,7270GA 7.8% 160.8 / 2.7 55G95S (GGC→AGC) proY_1Proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base A (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCCGGCCCGGCCATCATGTTGTCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCT  >  ACB122/1202593‑1202860
                                                                                                                                      |                                                                                                                                     
tGGCCGGCCCGGCCATCATGTTGTCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCg                                                                                                                                       <  2:127580/135‑1 (MQ=255)
tGGCCGGCCCGGCCATCATGTTGTCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGCGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCg                                                                                                                                       <  6:266495/135‑1 (MQ=255)
          ggccATCATGTTGTCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACTACGCTGTCGCCGGCTAGATCAGCCGTTATGCCCAGGATTACCTCGGGCCATTgga                                                                                                                             >  2:118611/1‑134 (MQ=255)
                  tgttggCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTcc                                                                                                                     <  6:42860/135‑1 (MQ=255)
                    ttgtCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTg                                                                                                                   >  2:22089/1‑135 (MQ=255)
                     tgtCGTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGa                                                                                                                  <  5:322789/135‑1 (MQ=255)
                       tCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGAcc                                                                                                                <  8:126645/135‑1 (MQ=255)
                         cTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCAGCCCATTGGCGGGCTTCCTGACCgg                                                                                                              >  1:40002/1‑135 (MQ=255)
                         cTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCAGCCCATTGGCGGGCTTCCTGACCGGc                                                                                                             >  7:272766/1‑136 (MQ=255)
                         cTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCATGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCgg                                                                                                              >  3:161987/1‑135 (MQ=255)
                             atcatcGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTg                                                                                                           <  6:153540/134‑1 (MQ=255)
                                atcGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCATCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAact                                                                                                      <  8:309041/136‑1 (MQ=255)
                                 tcGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAact                                                                                                      <  3:376289/135‑1 (MQ=255)
                                    gcggcCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAactact                                                                                                   >  4:337184/1‑135 (MQ=255)
                                         cTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTc                                                                                              >  5:381674/1‑135 (MQ=255)
                                            gCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCtg                                                                                           <  7:39816/135‑1 (MQ=255)
                                               aTTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCTGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTgg                                                                                        <  4:139512/135‑1 (MQ=255)
                                               aTTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTg                                                                                                  <  7:86809/125‑1 (MQ=255)
                                               aTTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTg                                                                                                  >  8:86809/1‑125 (MQ=255)
                                                 tCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCt                                                                                      <  3:207172/135‑1 (MQ=255)
                                                      tGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGt                                                                                   <  2:100294/133‑1 (MQ=255)
                                                               gcgcCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCAGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGc                                                                       <  8:272766/136‑1 (MQ=255)
                                                                cgcCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCAGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGc                                                                       <  2:40002/135‑1 (MQ=255)
                                                                     ttGGTGAAATGGCCGTGCACAACCCTGTCGCCGGGTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGTAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGaaa                                                                  <  3:345010/135‑1 (MQ=255)
                                                                     ttGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCCTTGGCGGGCGTCCTGCCCGGCTGGCACTACTGGTTCCTGTGGCTGGTGCCCTGTGTTGCCGaaa                                                                  >  2:181332/1‑135 (MQ=255)
                                                                     ttGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGaaa                                                                  <  5:73393/135‑1 (MQ=255)
                                                                     ttGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGaa                                                                   >  6:297114/1‑134 (MQ=255)
                                                                     ttGGTGAAATGGCCGGGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCGTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGaaa                                                                  <  4:6405/135‑1 (MQ=255)
                                                                           aaaTGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACgg                                                            <  7:210698/135‑1 (MQ=255)
                                                                                 ccGTGCACAACCCTGTCGCCGGCTCGTTCAGCCTTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGc                                                     <  8:15192/136‑1 (MQ=255)
                                                                                 ccGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTg                                                       <  6:381674/134‑1 (MQ=255)
                                                                                 ccGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTg                                                       <  4:159454/134‑1 (MQ=255)
                                                                                 ccGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTg                                                       <  3:337184/134‑1 (MQ=255)
                                                                                     gCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCAt                                                  >  5:162029/1‑135 (MQ=255)
                                                                                       acaACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGAGTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCt                                                <  1:22089/135‑1 (MQ=255)
                                                                                       acaACCCTGGCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCt                                                <  1:296591/135‑1 (MQ=255)
                                                                                            ccTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGGGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATg                                           <  2:9681/135‑1 (MQ=255)
                                                                                              tGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGCACTACTGGTTCCTGTGGCTGGGGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGc                                        >  7:374443/1‑136 (MQ=255)
                                                                                              tGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATgg                                          >  1:260/1‑134 (MQ=255)
                                                                                                gcGCCGGCTCGTTAAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGc                                        <  8:294655/133‑1 (MQ=255)
                                                                                                 cGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAATTCACGGCAGTGGCCATCTACATGGGCATc                                     >  7:117777/1‑136 (MQ=255)
                                                                                                 cGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCAt                                      >  5:203023/1‑135 (MQ=255)
                                                                                                   ccGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCAGAAATCACGGCAGTGGCCATCTACATGGGCATCt                                    >  6:221291/1‑135 (MQ=255)
                                                                                                      gCTCGTTAAGCCGTTATGCCCAGGATTACCTCCGCCCATTGGCGGGCTTCCAGACCGGCTGGAACGACAGGTTCCGGTGGCTGGTGACCTGTGTTGCCGAAATCCCGGCAGTGGCCCTCTACATGGGCATCGGGt                                 >  4:360266/1‑135 (MQ=255)
                                                                                                        tCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTc                               >  5:289765/1‑135 (MQ=255)
                                                                                                         cGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGCCCGGCTGGAACTACTGGTTCCGGTGGCTGGTGACCTGTGTTGCCGa                                                                    <  2:396091/97‑1 (MQ=255)
                                                                                                         cGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGa                                                                    >  1:215940/1‑97 (MQ=255)
                                                                                                         cGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGa                                                                    <  2:215940/97‑1 (MQ=255)
                                                                                                         cGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCGGTGGCTGGTGACCTGTGTTGCCGa                                                                    >  1:396091/1‑97 (MQ=255)
                                                                                                              aGCCGTTATGCCCAGGATTACCTGCGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGAc                         <  2:377434/135‑1 (MQ=255)
                                                                                                                      tGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCcgc                  <  2:236310/134‑1 (MQ=255)
                                                                                                                        cccAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCt               >  3:332680/1‑135 (MQ=255)
                                                                                                                         ccAGGATTCCCGCGGCCCCTTGGCGGGCTTCCTGCCCGGCTGGAACTCCTGGTTCCGGTGGCTGGGGACCGGTGTTGCCTAACTCACGGCAGTGGCCATCTACATGGGCATCTGGGTCCCCGACGTTCCGCGCTg              <  5:233930/135‑1 (MQ=255)
                                                                                                                           aGGATTACCTCGGCCGATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTGCCTGTGGCTGGGGACCTGTGTTGCCGAAATCTCGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGAt           <  7:307774/136‑1 (MQ=255)
                                                                                                                             gATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATc          >  3:143879/1‑135 (MQ=255)
                                                                                                                                tACATCGGCCCATTGGCGCGCTTCCTGACCGGCTGGATCTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCAGAACTCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTgg       >  4:390510/1‑135 (MQ=255)
                                                                                                                                     cGGCCCATTGGCGGGCTTCCTGCCCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCCTCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCt  >  3:320133/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TGGCCGGCCCGGCCATCATGTTGTCCTACATCATCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCT  >  ACB122/1202593‑1202860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑