| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 96,480 | T→G | 25.7% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 96,480 | 0 | T | G | 25.7% | 68.3 / 2.4 | 62 | intergenic (+121/‑72) | cynT/CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/25); new base G (13/3); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.87e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTGG > ACB122/96348‑96609 | ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc < 1:375510/135‑1 (MQ=255)ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc > 1:44353/1‑135 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCGTGAATTCCCTGACAGCCCTGCCGGCCTGCTCGCTGGCGGCCCGCCCCGGCGCCCtg > 4:259104/1‑134 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGCCGTGAATTCCCTGACTGCCCTGCCGGCGTGCGCGCTGGCGGCCCGCGCCGgcgcgggt > 6:351302/1‑132 (MQ=255) ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGt > 3:162587/1‑135 (MQ=255) gCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCCGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAg < 1:314205/134‑1 (MQ=255) gACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCCCCGGCCCGCGTGTCAgggg > 4:337013/1‑135 (MQ=255) cccGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGCCGTGAATTACCTGCCTGCCCTGCCGGCGTGCGCGCTGGCGGCCCGCGCCGGCGGGCTTGGGAGGGGcg > 7:347381/1‑134 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGGTGCCTTGTATTACCGGCCTGCCCAGCCCGCGGGGACGCTGGCGGCCCCCCCCCGGGCGCGTGTGAcgggggaac > 6:48279/1‑130 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGGGGCCTTGAATTACCTGACTGCCCAGCCGGCGTGCTCGCTGGCGGCCCCGCCCCGCGCGCCTGTGAgggggcacc > 6:11830/1‑130 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACg > 7:303225/1‑136 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGTGAGGGGCAAACg > 8:213846/1‑136 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGTCAGGGGCAAACg > 8:116533/1‑136 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCaaaa > 2:2085/1‑134 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCAAACg > 8:275095/1‑136 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCGTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGGCGGGGGGCGTGTCAgggggcaac > 1:93523/1‑130 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCGTGAATTCCCTGACAGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCGCCAGCGCGCGTGTCACGGGCAAAc > 2:39427/1‑135 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCGGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCCAACg > 7:192558/1‑136 (MQ=255) cgccgcTAGAACCTTGATAGCCGGCTGCACCCTTCCGGGGTGTGGCGCGGCCTTCGGCGGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGGTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGAAAACGTg < 2:239758/135‑1 (MQ=255) cgccgcTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTg < 2:240168/135‑1 (MQ=255) cgccgcTAGAACCTTGATAGCCGGCTGCACCCTGCAGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGGGCGCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGtc < 2:367762/135‑2 (MQ=255) aGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCGCGCTGGCGGCCCCCCCCGGCCCGCGTGTCAGGGGCCAACGGGccccccg > 3:77990/1‑131 (MQ=255) gcggCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTg < 8:347381/133‑1 (MQ=255) cACCCTTCAGGGGGGGGGCGCGGCCTGCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGGGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGa < 5:133735/135‑1 (MQ=255) gggTGTGGCGCGGCCGTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGG‑CCGCGCCTGCGCGCTTGTCCGGGGCAAACGTGCCCACGGCCAGGGGCAAGGCCGTGCGACTACAGaaa > 6:153935/1‑133 (MQ=255) gcgcGGCCTTGGGCTGCGTTGAATTCCCTGAGGGCCGTGCGGGGGTGCGGGCGGGGGGCCCGCGCGTGCGGGGTTGTCGGGGGAAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAac < 5:337291/135‑1 (MQ=255) cgcgGCTTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCGGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:275095/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTTCCCTGCCGGCGTGCTCGCTGGCCGCCCGCGGCTGCGCGCATGTAAGGGGCAAAAGTTCCCACCGGCACGGGAAATGCCCGGCGAATAGAAAAAGGGGAAaccc > 8:71959/1‑134 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGGTGGCGGCCCGCGCCTGCGCGGTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:8397/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTGAGGGGCAAACGTGCCCACGGCCAGGGGGATGGCCGTGCGACGAGCGCAAGGAGCAacac > 8:181110/1‑136 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGGATGGCCGTGCGACTAGAGAAAGGAGTAacac > 7:233619/1‑136 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGTCAGGGGCAAACGTGCCCACGGCCCGGGGCATGGCCGTGCGACGAGAGAAAGGAGCAacac > 8:114343/1‑136 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTGAGGGGCAAACGGGCCCCCGGCCCGGGGGATGGCCGTGCGACTATAGAAACGAGCAacc > 2:98887/1‑134 (MQ=255) cgcgGCCTGGGGCTGCCTTGAATTCCCTGACTGCCCTGCGGGCGTGCGCGGTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:245047/136‑1 (MQ=255) gcgGCCTTCGGCTGCCTTGAATACCCTGACTGCCCTGCCGGCGGGCGCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 4:237131/135‑1 (MQ=255) cGGCTGCCTTGAAATCCCTGACGGCCCTGCCGGGGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGCCCGGGGCAACCGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACa < 3:45599/135‑1 (MQ=255) ggttGCCTTGAATTCCGTGACTGCCCTGCCGGCGTGCTCGGTGGCGGTCGGGGACTGGGGGCTTGTGAGGGGCAAACGTGCCGACGGCCAGGGGAATGGCCGTGAGACTAGAGAAAGGAGAAACACGGTGAACAtt < 7:330101/133‑1 (MQ=255) gggtGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 7:71959/133‑1 (MQ=255) ggCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 7:181110/136‑1 (MQ=255) gCTGCCTTTAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGGGGCCCGCGCGTGGGCGCTTGTCAGGGGAAAAGGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 1:2085/135‑1 (MQ=255) gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 4:162587/135‑1 (MQ=255) gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 2:44353/135‑1 (MQ=255) gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCTGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 3:337013/135‑1 (MQ=255) gCTGCCTTGAATGCCCTGCCGCCCCGGCGGGGGGGCTCGGGGGCGGCCGGCGCGGGCGGGGTTGGCGGGGGAAAAGGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 3:259104/135‑1 (MQ=255) gCTGCCTTGAAATCCCTGACTCCCCTGCGGGGGTGCTCGCTGGGGGCCCGCGCCTGGGCGGTTGTCTGGGGCAAAGGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACTGTGAACAtt < 4:84024/135‑1 (MQ=255) gCTGCCTTCAATTCCCTGACTGCCGTGCCGGCGTGCTGGCGGGCGGCCCGCGCCTGCGCGCTTGTCTGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 4:33428/135‑1 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGtt > 8:94085/1‑136 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGGCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGGCGAGAGAAAGGAGAAACACGGTGAACATTACACAGt > 2:103875/1‑135 (MQ=255) aTTCCTTTACGGCCCTGCCGGCGGGGGCGGGTGGGGCCCGCGCCTGCGCGGTTGGCAGGGGCAAACGTGCCCACGGCATGGTTAATGGCCGTGCGACTAGAGAAAGGATAAACACGGTGAAAATTACAGAGTTGa < 8:230862/135‑1 (MQ=255) gACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGcc > 7:123613/1‑136 (MQ=255) ctgccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt > 7:378334/1‑109 (MQ=255) ctgccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt < 8:378334/109‑1 (MQ=255) tgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGGCAGGGGCATGGCCGGGCGCCTAGAGAAAGGAGCAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCcgc > 6:89537/1‑135 (MQ=255) tgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:399109/100‑1 (MQ=255) tgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac > 8:399109/1‑100 (MQ=255) gCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGGGCGACTAGAGAAAGGAGAAACCCGGTGAACATTACACAGTTGAAAGCGGCCCTGCcgctgggt > 1:242620/1‑130 (MQ=255) cTCGCTGGCGGCCCGCGCCTGCGCGCGTGTGAGGGGCAAACCTGCCCCCCGCCAGGGGCATGGCCGTTCGACGTGAGAAATGAGAAAAACGGTGCACATTTCACAGTTGAAAGCGGCCCTGCCGCGGGCGTTGCt > 6:225733/1‑135 (MQ=255) cTCGCTGGCGGCCCGCGCCTGCGCGCGTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCGGCCGCGTGAGGTGCt > 5:304170/1‑135 (MQ=255) cgggggcggcCGGCGCCGGCGCGCTTGGCCGGGCCAACCGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAACGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTgg < 5:187991/131‑1 (MQ=255) cgcgCCTGCGCGCGTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGGGCGACTAGAGAAAGGAGACACCCGGTGAACATTACACAGTTGAACGCGGCCCTGCCGCGTGAGGTGCTGGCGTCggggggg > 4:57061/1‑133 (MQ=255) gcgcGTGCGGGTTTGCCAGGGGCAAACGTGCCAACGGCCTGGGGAATGGCGTTGCGACTAGATAAAGGAGAAACACGGTTAACATTACACAGTTGAACGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtggtgg < 6:176623/135‑1 (MQ=255) gcgcCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGCCTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGGGAGTTGCGGGCGTGggtggtgg > 4:45950/1‑135 (MQ=255) | GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTGG > ACB122/96348‑96609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |