Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,205,992 A→G 7.8% P1340P (CCT→CCC)  rhlB_1 ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,205,9920AG 7.8% 131.9 / 4.6 53P1340P (CCT→CCC) rhlB_1ATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base A (28/18);  new base G (2/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03

TTGCCAATCACTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCAA  >  ACB122/1205858‑1206123
                                                                                                                                      |                                                                                                                                   
ttGCCAATCACTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCa                                                                                                                                     <  4:169078/135‑1 (MQ=255)
       tCACTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGc                                                                                                                             <  7:236637/136‑1 (MQ=255)
        cACTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGc                                                                                                                             >  8:114388/1‑135 (MQ=255)
          cTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGa                                                                                                                           <  6:11531/135‑1 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCCCTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTAACCGGcc                                                                                                             >  5:65432/1‑135 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGCACTGTTCCCCGGc                                                                                                              >  8:183263/1‑134 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCgg                                                                                                               >  6:353784/1‑133 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCa                                                                                                            >  8:131139/1‑136 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCa                                                                                                            <  7:67860/136‑1 (MQ=255)
                        gCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCGCCGGCAAGGCGAACTGTTCCCCGGcc                                                                                                             >  2:114303/1‑135 (MQ=255)
                                 agcaACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCCCCGCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCa                                                                                                    >  1:252900/1‑135 (MQ=255)
                                      cgagccgagcAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAg                                                                                              <  7:184198/136‑1 (MQ=255)
                                           cGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGcgcc                                                                                          >  4:175909/1‑135 (MQ=255)
                                           cGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGCTTCACGTCGTCTCACATGCCGCAGCCATACCACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGCAGcgcc                                                                                          >  6:282916/1‑135 (MQ=255)
                                            gATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTAACGTCGTCTCACATGACGCAGCAATACTACCCCCGCACGCAAGGCGCACGGTTCACCGGCCAGCCCGGGAATGAAGcgccc                                                                                         >  2:199537/1‑135 (MQ=255)
                                             agcAGGTTCAGCGGGGCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGcgcccg                                                                                        <  6:264034/133‑1 (MQ=255)
                                             aTCAGGTTCAGCGGGGCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGcgcccg                                                                                        <  3:82208/135‑1 (MQ=255)
                                                  gTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCg                                                                                   <  4:345541/135‑1 (MQ=255)
                                                          ggTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCa                                                                                                                                     <  3:134812/77‑1 (MQ=255)
                                                          ggTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCa                                                                                                                                     >  4:134812/1‑77 (MQ=255)
                                                           gTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCACACATGCCGCATCAATACTACCGCCTCAGTCAATGCGAACAGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCCGATCTCGcc                                                                          >  1:333506/1‑135 (MQ=255)
                                                               ccGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCAcgc                                                                      >  4:312909/1‑135 (MQ=255)
                                                                    gcTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTc                                                                 <  4:75131/135‑1 (MQ=255)
                                                                        accaccTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCCGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCCGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACCCGCGTCCCAt                                                             >  4:204417/1‑135 (MQ=255)
                                                                        accaccTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAAt                                                             >  4:96075/1‑135 (MQ=255)
                                                                        accaccTGCAGCGTTCCCTCCGGTTCCCGTCGTCTGACTTTGCGCACCCATAATACCGCCTCAAGCAAGGCGCCCAGTTCACCGGGCCGCCCCGCCATGAAGCGCCCGCCCCGGAGCACCCCCCGCGCGTCGAAt                                                             >  2:183007/1‑135 (MQ=255)
                                                                                     ttCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCGGGCAAGGc                                                                                                                             >  3:35113/1‑58 (MQ=255)
                                                                                     ttCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCGGGCAAGGc                                                                                                                             <  4:30060/58‑1 (MQ=255)
                                                                                     ttCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCGGGCAAGGc                                                                                                                             >  3:30060/1‑58 (MQ=255)
                                                                                     ttCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCGGGCAAGGc                                                                                                                             <  4:35113/58‑1 (MQ=255)
                                                                                      tccgtccgGTTCAGGTCGTCTCACTTGCCGCAGAAATACAACCGCCTCAGCCAAGGCGAACTGTTCACCGGCCAGCCCGGAAATGAAGCGCCCGCCCCGGATCTCGCAACGCGCTTCCAATCGGTGATAgcagcg                                               <  5:292504/135‑1 (MQ=255)
                                                                                      tccgtccgGATCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCACCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAgcagcg                                               <  2:44565/135‑1 (MQ=255)
                                                                                       ccgtccgGTTCGCGTCGTCTCACTTGCCGCAGCACTACAACCGCCTCCGGCAACGCGAACTGGTCACCGGCCCGCCCGGCCATGAAGCGCCCGCCCCGGATCTCGCCACGCGCGTCCAGTCGGTGATagccgc                                                >  5:263872/1‑133 (MQ=255)
                                                                                       ccgtccgGTTCACGTCGTCTGACTTTGCGCAGCAATACTACCCCCTCAGGCAAGGCGGACGGTTAACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCCGCTCTCGCCCCCCGCGTCCAATCGGTGATagccgc                                                >  6:292504/1‑133 (MQ=255)
                                                                                       ccgtccgGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATagcagc                                                >  1:313662/1‑133 (MQ=255)
                                                                                       ccgtccgGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCGCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATagcagc                                                >  3:344400/1‑133 (MQ=255)
                                                                                          tccgGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCCCGCGCTTCCAATCGGTGATAgcagcgca                                             >  1:210560/1‑133 (MQ=255)
                                                                                                tCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCAc                                    >  8:136651/1‑136 (MQ=255)
                                                                                                 cACGTCGGCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCAc                                    <  2:2345/135‑1 (MQ=255)
                                                                                                  aCGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGACCTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGCTCTCGCCCCGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACg                                   >  1:314067/1‑135 (MQ=255)
                                                                                                  aCGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGCTCTCGCCACGCGCGGCCAATCCGTGATAGCAGCGCAGCAGCTCACg                                   >  6:9550/1‑135 (MQ=255)
                                                                                                  aCGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGc                                  >  7:191240/1‑136 (MQ=255)
                                                                                                  aCGTCGTCTCAATTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCGTCCATACCGTGTTAGCAGCGCAGCAGCTCACg                                   >  6:317073/1‑135 (MQ=255)
                                                                                                     tcgtcTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCa                                <  3:96075/135‑1 (MQ=255)
                                                                                                     tcgtcTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCa                                <  2:18845/135‑1 (MQ=255)
                                                                                                       gtcTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAggg                             <  7:114388/136‑1 (MQ=255)
                                                                                                        tctcACTTGCCGCAGCAATACTACCGCCTCCGGCAAGGCGAACTGTTCACCGGCCAGCCCGCCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAggg                             <  3:32395/135‑1 (MQ=255)
                                                                                                         ctcACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGggc                            <  2:251863/135‑1 (MQ=255)
                                                                                                            aCTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCATCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGggcg                           >  2:292175/1‑133 (MQ=255)
                                                                                                            aCTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGggcgg                          <  7:131139/134‑1 (MQ=255)
                                                                                                                     agcaATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGGTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCACTACATCa                >  2:308338/1‑135 (MQ=255)
                                                                                                                               ccGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCgcgcgc     <  7:136651/136‑1 (MQ=255)
                                                                                                                                  ccTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCaa  >  8:229767/1‑136 (MQ=255)
                                                                                                                                  ccTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCaa  >  8:369386/1‑136 (MQ=255)
                                                                                                                                  ccTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCa   >  3:105081/1‑135 (MQ=255)
                                                                                                                                  ccTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCa   <  3:280618/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TTGCCAATCACTGCCGGCACTTTCGCCCCAGGCAGCAACGAGCCGATCAGGTTCAGCGGGTCACCGGCGCTCACCACCTGCAGCGTTCCGTCCGGTTCACGTCGTCTCACTTGCCGCAGCAATACTACCGCCTCAGGCAAGGCGAACTGTTCACCGGCCAGCCCGGCAATGAAGCGCCCGCCCCGGATCTCGCCACGCGCTTCCAATCGGTGATAGCAGCGCAGCAGCTCACGCCAGGGCGGCAGTACATCACTCTCGCGCGCCAA  >  ACB122/1205858‑1206123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑