Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,464,306 T→G 17.9% Q16P (CAG→CCG)  CJ019_01381 ← Mannuronan synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,464,3060TG17.9% 81.7 / 2.3 53Q16P (CAG→CCG) CJ019_01381Mannuronan synthase
Reads supporting (aligned to +/- strand):  ref base T (17/25);  new base G (6/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACCGACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCG  >  ACB122/1464172‑1464433
                                                                                                                                      |                                                                                                                               
ccACCGCCTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCTGGCGTGTTGGCGCTg                                                                                                                                >  7:180439/1‑136 (MQ=255)
ccACCGACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGGGCt                                                                                                                                 >  1:274278/1‑135 (MQ=255)
ccACCGACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGGGTTGGCGCt                                                                                                                                 >  4:203888/1‑135 (MQ=255)
  acggACTGCGGTTGCTGGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGAGTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTgg                                                                                                                               <  6:345706/132‑1 (MQ=255)
   ccGACTGGGGTTGCTTGGGGGGGACGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTggg                                                                                                                              <  1:373628/135‑1 (MQ=255)
   ccGACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTTGCGGGAATGCGTAGCCGGGCGTGGTGGGGCTggg                                                                                                                              >  4:157995/1‑135 (MQ=255)
     gACTGCGGTTGCTTGGTGTGGAAGCTCAGACCTCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGcg                                                                                                                            >  6:86302/1‑134 (MQ=255)
     gACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGGTGGCGCGGGGcgt                                                                                                                           >  7:37534/1‑134 (MQ=255)
        tGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGCCCTTGACATCGTGGACCTGGCGCTGGGCGTCCACGCACCGCAGCATGGCGGGAATGAGTAACCGGGCGGGGTGGGGCCgggggtgg                                                                                                                         >  4:300167/1‑130 (MQ=255)
          cGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGa                                                                                                                       >  3:167048/1‑135 (MQ=255)
             ttGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCGTCgggcgca                                                                                                                   >  7:134930/1‑131 (MQ=255)
               gCTTGGTGTGGACGCGCAGACCGCCGGAAGAAAGGTCTTCGACCTTGACTGCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  1:393810/135‑1 (MQ=255)
                cTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  1:117143/134‑1 (MQ=255)
                cTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  4:115266/134‑1 (MQ=255)
                cTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  2:245350/134‑1 (MQ=255)
                cTTGGTGTGGAAGCGCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  3:300167/134‑1 (MQ=255)
                     tgtgGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGGTGGGGCGGGGCGTCGGCCTCATGCAcc                                                                                                             >  5:187791/1‑133 (MQ=255)
                      gtgGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCTGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACa                                                                                                          <  8:180439/136‑1 (MQ=255)
                        ggAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACa                                                                                                          <  4:365374/134‑1 (MQ=255)
                           agatCACACCGCCGGCAGAATGGTCTTCGACGTTGACTTCGTGGACCGGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAAGGCGTACCCGGGCGTGTGGGCGCTGGGCTGCGGACTCATGCACGACATTGAc                                                                                                     <  8:373435/133‑1 (MQ=255)
                             cTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  >  7:10593/1‑121 (MQ=255)
                             cTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  8:10593/121‑1 (MQ=255)
                                aGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCGTCGGACTCAATCACCACATTTACGGTc                                                                                                 >  4:286308/1‑135 (MQ=255)
                                  aCCGCCGGCAGAATGGGTTTCGACCTTGACTGCGTGGACGTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTGGGACTCATGCACGACATTGACGTTCAc                                                                                               <  3:286308/135‑1 (MQ=255)
                                  aCCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGGCTCATGCACGACAttgacgttgacg                                                                                              >  7:31388/1‑136 (MQ=255)
                                  aCCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACACATGCCCGACATTGACGTTCAcg                                                                                              >  8:5768/1‑136 (MQ=255)
                                   ccgccgGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCAcg                                                                                              <  4:167048/135‑1 (MQ=255)
                                   ccgccgGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCAcg                                                                                              <  3:203888/135‑1 (MQ=255)
                                     gccgGCATAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCTGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGAGGTGCAcggc                                                                                            <  4:387894/135‑1 (MQ=255)
                                         gCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTTGCGCGGGGCTTCGGACTCAAGCACGCCCTTGACGTTGACGGCGGtt                                                                                        >  6:285271/1‑134 (MQ=255)
                                                ggTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGGGTGTTGGCGCTGGGCTTCGGAGTCATGCAGGACATTGACGTTCACGGCGGTATTCAtggt                                                                                <  8:202849/136‑1 (MQ=255)
                                                ggTCTTCGACCTTGACTTCGTGGACCTGGCGCGGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCGGGGCTTCTGACTCATGCACGACATTGCCGTTCACGGCGGTATTCAtggt                                                                                <  8:28685/136‑1 (MQ=255)
                                                 ggtttCGCCGTTGACTTGGGGGAGGGGGGGGTGGGGGTCCTGTAACCGCCGGTTGGCGGTATTGGGTACCGGGGCGTGTGGGCGCGGGGGTCGGGCCTCATCCACGACATGGACGTTCACGGCGGTATTCAtggt                                                                                <  2:198466/132‑1 (MQ=255)
                                                 gTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCAtggt                                                                                <  3:93990/135‑1 (MQ=255)
                                                 gTCTTCGACCTTGACTTCGTGGACCTGGCGCGGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCGGGGCGGGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTCCACGGCGGTATTCAtggt                                                                                <  4:71998/135‑1 (MQ=255)
                                                       gACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCGTCGGAGTCCTGCACGACAATGACGGTCCCCGGGGGATTGATGGTGGGTTg                                                                          >  5:130990/1‑135 (MQ=255)
                                                       gACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGGTGGCGCTTGGCGTCGTGCGCATGGAAGCCATTGACGTTCACGGAGGTATTCAtggtgtgttg                                                                          >  1:123448/1‑130 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGCCTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGt                                                                   >  7:243334/1‑134 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  <  7:391778/87‑1 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCAt                                                                                                                  >  8:391778/1‑87 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTc                                                                                     >  7:375178/1‑116 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTc                                                                                     <  8:375178/116‑1 (MQ=255)
                                                               cTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCGGGGCTTCGGACTCATGCACGCCATTTACGTTCACGGCGGTATTGATGGTGGTTTGTTGCTGtt                                                                  >  1:64064/1‑135 (MQ=255)
                                                                     ggACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCGGGGCGTCCGACTCCTGCCAGACATTTTCGGTCCAGGGGGTATTCATCGTGGGTTGGTCCGGGTAggacc                                                            >  2:311474/1‑131 (MQ=255)
                                                                       aCCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGGTGGCGCTGGGGTTCGGACTGATGCACGCCATTTACGTTGCCGGCGGTATTCATGGTGGTTTGTTCCTGTTAgatccgct                                                         >  8:269479/1‑134 (MQ=255)
                                                                          tGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCGGGGCGGGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAgatccgatcc                                                       <  3:383544/135‑1 (MQ=255)
                                                                                     gcccAGTAACGGAAGCTGGGGGGTAATGCTTCCCGGGGGGTGGGGGCGGGGGGCTGCGGACTCTTCCACGAAATTGAGGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTc                                            <  2:274278/133‑1 (MQ=255)
                                                                                     gTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGGTGGGGGGGGGGGTGGGGCTCATGCACGCCAATGACGTTGACGGCGGGATTCATGGGGGTTTGTTGGGGGTAGATCCGATGCGGGGTGGGCTca                                           >  7:85886/1‑136 (MQ=255)
                                                                                                   ggttGGGGGGAATGCGTACCCGGGCGTGTGGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAaca                             <  8:85886/134‑1 (MQ=255)
                                                                                                   gCTGGGCGGGAATGCGTCCCCGGGCGTGTGGGCGCTGGGCTTCGGACTCATGCACGAAATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAaca                             <  8:243334/136‑1 (MQ=255)
                                                                                                    cTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAaca                             <  3:382435/135‑1 (MQ=255)
                                                                                                            gAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGCCATTGACGTTCCCGGCGGTTTTAATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCaa                    >  7:64017/1‑136 (MQ=255)
                                                                                                               tGCGTACCCGGGCGTGTTGGCGCTGGGCTGCGGACTCATCCACCACATTGCCGTCCACGGCGGTATTCATGGGGGTTTGTACCTGTTCGATCCGAGCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACg                  <  2:55787/135‑1 (MQ=255)
                                                                                                                            gtgtTGGCGCTGGGCTTCGGACTCATGCACGACATTTACGTTCACGGCGGTATTCATGGTGGGTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACCCGACCATGAACAGCACAGCAACGAAGATGCTGGCAg     >  5:262968/1‑135 (MQ=255)
                                                                                                                                tGGCGCGGGGCTTCGTACTCATGCACGACATTGACGTGCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCg  <  2:64064/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                               
CCACCGACTGCGGTTGCTTGGTGTGGAAGCTCAGACCGCCGGCAGAAAGGTCTTCGACCTTGACTTCGTGGACCTGGCGCTGGGCGTCCAGGAACCGCAGCTTGGCGGGAATGCGTACCCGGGCGTGTTGGCGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCG  >  ACB122/1464172‑1464433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑