Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,503,076	G→A	69.1%	P516L (CCG→CTG)	lpoA ←	Penicillin‑binding protein activator LpoA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,503,076	0	G	A	69.1%	90.6 / 57.5	68	P516L (CCG→CTG)	lpoA	Penicillin‑binding protein activator LpoA
Reads supporting (aligned to +/- strand):  ref base G (11/10);  new base A (20/27);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGTTGGCCCAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGCTTGATC  >  ACB122/1502942‑1503204                                                                                                                                       |                                                                                                                                 tCAGTTGGCCCAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACg                                                                                                                                  >  1:222664/1‑135 (MQ=255) tCAGTTGGCCCAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGACGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACgg                                                                                                                                 >  8:18025/1‑136 (MQ=255)          ccAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGCCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTCTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGAGTTTc                                                                                                                           >  2:158460/1‑133 (MQ=255)           cAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTc                                                                                                                           >  6:256543/1‑132 (MQ=255)            aGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGa                                                                                                                      <  7:283679/136‑1 (MQ=255)                gcggcgcCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGc                                                                                                                   >  5:215105/1‑135 (MQ=255)                 cggcgcCAGGCTGTAGGCATCGACGCCCATGGCATACCGGCGGCCGCGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCGGTTGACGCAGGCTGGTGCTGGTGTCGCGCAGCCACGGTGTTTCGCAGAAGCg                                                                                                                  >  5:111771/1‑135 (MQ=255)                  ggcgcCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCgg                                                                                                                 <  5:127982/135‑1 (MQ=255)                      ccAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGc                                                                                                             >  1:126883/1‑135 (MQ=255)                        aGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGt                                                                                                          <  7:49912/136‑1 (MQ=255)                         ggCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGt                                                                                                          <  4:391158/135‑1 (MQ=255)                         ggCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGt                                                                                                          <  2:110170/135‑1 (MQ=255)                         ggCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGt                                                                                                          <  3:8286/135‑1 (MQ=255)                                 gCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGTCTTGCGGCCACTGCTGTACACCCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCa                                                                                                  >  3:38490/1‑135 (MQ=255)                                 gCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCa                                                                                                  >  1:174754/1‑135 (MQ=255)                                   aTCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTAGAACCTGGTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCAtt                                                                                                <  6:342793/135‑1 (MQ=255)                                   aTCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCAtt                                                                                                <  5:189461/135‑1 (MQ=255)                                    tCGACGCCCATGGGATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGTCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGGGTTTCGCAGAAGCGGATGCCGTTCATGTCATTg                                                                                               <  5:242124/135‑1 (MQ=255)                                        cGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACg                                                                                                                                  >  3:276811/1‑96 (MQ=255)                                        cGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACg                                                                                                                                  <  4:276811/96‑1 (MQ=255)                                        cGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACt                                                                                           <  4:320235/135‑1 (MQ=255)                                        cGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTg                                                                                          >  7:198638/1‑136 (MQ=255)                                         gCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTg                                                                                          <  4:278187/135‑1 (MQ=255)                                                   tACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCAc                                                                               <  8:236609/136‑1 (MQ=255)                                                    aCAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCAc                                                                               <  4:308484/135‑1 (MQ=255)                                                    aCAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCAc                                                                               <  1:194093/135‑1 (MQ=255)                                                    aCAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCAc                                                                               <  2:196041/135‑1 (MQ=255)                                                       ggcggcCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGc                                                                            <  4:177763/135‑1 (MQ=255)                                                        gcggcCGAGGCTGCCGGCGGCTTGCGTCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTg                                                                          <  7:288771/136‑1 (MQ=255)                                                         cggcCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTg                                                                          <  4:314598/135‑1 (MQ=255)                                                         cggcCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTg                                                                          <  2:222664/135‑1 (MQ=255)                                                         cggcCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTg                                                                          <  3:275993/135‑1 (MQ=255)                                                         cggcCGAGGCGGCCGGCGGCTTGCGGCCACTTCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTg                                                                          <  2:88923/135‑1 (MQ=255)                                                           gcCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGgc                                                                        >  4:219511/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGTTTGATGTCACCGCTGGCGCt                                                                     >  1:150579/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  6:275358/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTg                                                                    >  7:190591/1‑136 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGCTGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  3:392168/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  4:297002/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  6:247250/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  1:155428/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  2:325550/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     >  4:328354/1‑135 (MQ=255)                                                              gAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCAAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCt                                                                     <  4:330944/135‑1 (MQ=255)                                                               aGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTg                                                                    <  1:165313/135‑1 (MQ=255)                                                                    gCCTGCGGCTTGCGGCCTCTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGGGTCGAGCAGCCTCAGTGTTTCGCAGTAGCGGAAGGCGATCATGGCATTGTACTGGTGGATGTCACCTCTGGGGCTGGACAg                                                               <  1:158460/135‑1 (MQ=255)                                                                        gcggcTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGCTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTc                                                           <  3:69758/135‑1 (MQ=255)                                                                         cggcTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCg                                                          <  3:51459/135‑1 (MQ=255)                                                                               gCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTg                                                     >  2:394662/1‑134 (MQ=255)                                                                                   ccACTGCTGTACAACCTTTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTGATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCTATGTGGCATa                                                <  2:122030/135‑1 (MQ=255)                                                                                          tgTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCg                                           <  2:148424/133‑1 (MQ=255)                                                                                          tgTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGCGGTGGCATAGACCg                                           <  6:324607/133‑1 (MQ=255)                                                                                               aaCCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGt                                     <  5:247250/134‑1 (MQ=255)                                                                                                    gTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGCCCGGCACGTCACCGGc                              >  8:216620/1‑136 (MQ=255)                                                                                                      tGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTc                                                           >  8:228530/1‑105 (MQ=255)                                                                                                      tGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTc                                                           <  7:228530/105‑1 (MQ=255)                                                                                                      tGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGc                              >  3:100876/1‑134 (MQ=255)                                                                                                       gACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGt                            >  6:363534/1‑135 (MQ=255)                                                                                                       gACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGt                            >  3:353694/1‑135 (MQ=255)                                                                                                       gACGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGt                            >  3:347870/1‑135 (MQ=255)                                                                                                         cGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTAc                          >  4:391733/1‑135 (MQ=255)                                                                                                         cGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTAc                          <  2:343726/135‑1 (MQ=255)                                                                                                         cGCAGGCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTAc                          >  6:88182/1‑135 (MQ=255)                                                                                                              gCTGTTGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGaa                     <  5:88182/135‑1 (MQ=255)                                                                                                                   tGCTGGTGTCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCa                <  1:12190/135‑1 (MQ=255)                                                                                                                     cTGGTGTCGAGCAGCCACAGTGTTTCGAAGAAGCGGATGCCGGTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAg               <  5:256543/134‑1 (MQ=255)                                                                                                                        gtgtCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTc           <  7:198841/135‑1 (MQ=255)                                                                                                                           tCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGc        >  1:89555/1‑135 (MQ=255)                                                                                                                           tCGAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGc        >  4:113332/1‑135 (MQ=255)                                                                                                                             gAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGCtt      >  6:370165/1‑135 (MQ=255)                                                                                                                             gAGCAGCCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGCTTg     >  8:98591/1‑136 (MQ=255)                                                                                                                                 agcCACAGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGCTTGATc  <  3:328354/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 TCAGTTGGCCCAGGCGCGGCGCCAGGCTGTAGGCATCGACGCCCATGGCATACAGGCGGCCGAGGCTGCCGGCGGCTTGCGGCCACTGCTGTACAACCTGTTGACGCAGGCTGTTGCTGGTGTCGAGCAGCCACGGTGTTTCGCAGAAGCGGATGCCGTTCATGTCATTGTACTGGTTGATGTCACCGCTGGCGCTGTACAGGTTCGAGGTGGCATAGACCGGCACGTCACCGGCGTACTGGAAGTTCAGGGTCGGCTTGATC  >  ACB122/1502942‑1503204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑