| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,585,043 | T→G | 32.3% | intergenic (‑94/‑109) | rhaS_1 ← / → CJ019_01492 | HTH‑type transcriptional activator RhaS/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,585,043 | 0 | T | G | 32.3% | 25.0 / 3.1 | 32 | intergenic (‑94/‑109) | rhaS_1/CJ019_01492 | HTH‑type transcriptional activator RhaS/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (5/14); new base G (8/2); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCC > ACB122/1584912‑1585176 | aGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTg < 6:391892/133‑1 (MQ=255) cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCGGCAGGa > 1:271626/1‑133 (MQ=255) tcggttcggtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGAAACAGCCCCCCAGCAGGCACAGGc > 5:394967/1‑134 (MQ=255) tagctGGACGTGGCGGTGGTCATGATGGCTATCCTATGCTGACCGACACATCATAGATTTGGGCAAAGACGCGCTCTAGTCGAAAGATGCATTTGGAATGCAATATACAGCCCCCCAGCAGGAACAGGCAATCGa > 4:155169/5‑135 (MQ=255) aCGGGGCGGTGGTCATGATGGCTTCCCTCTGCTGACCGACGCAGAATAGGTTTGGGCAACGACGGGCGCTAGTCTAAAGCGGCATGTGGATTGCCTGATCCGGCCGCGCTGCAGGATCAGGCAATCGACATGCAg < 6:394967/135‑1 (MQ=255) cGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGATACTTCCCCCCCGCACGCTCAGGCAATACACATGCAGt > 7:81021/1‑135 (MQ=255) ggtggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGATACTGCCGCGCTGCAGGCTCAGGCAATCGACAGGCAGTAATgg > 6:319458/1‑134 (MQ=255) atgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATAGACAAGCAGTAAATGCCTAAcgcgcc > 4:307584/1‑135 (MQ=255) tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCGGGTACTGCCCCGCGGCCGGGTCCGGCCATCGCCATGAAGTAATGGCCCAACCCGCCGCGCGGGTGAg > 8:217846/1‑136 (MQ=255) tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCGGATCCTGCCGCGCGGCCCGCTAAGGGAATCGCCAGTCACTAAAGGGCGAACCCCCCGCGCgggggg > 3:374774/1‑132 (MQ=255) gaggcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGCTCCGGCCGCGCGGGCGGCTCAGGCCATCGACCTGGAGTTATGGCCTAACGCGCCCCGCGGGTGGGCGCCGAAcc > 3:198926/4‑135 (MQ=255) gACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGCAACAGCCCCGCGGCACGTTGCGGCAATCGACAAGCACTAATGGCCGTACGCGCCGCGCGGGTGCGCGCCGAAcca > 8:296410/1‑135 (MQ=255) agcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGATCAGGCCGCGCGGGAGGCTCAGGCAAACGACATGCAGTATTTGCCTAACGCGCCGCGCGGGGGGGCGCCGAACCGTGGc > 6:317711/1‑135 (MQ=255) gcaTAGGTTTGGGCAAAGACGGGCGCTAGTCTAAAGCTGCATGTGGATTGCCTGATCCGGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCa < 6:167158/135‑1 (MQ=255) tGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATAGCCTGATACTGCCGCGCGGCAGGTTCAGGCCATCGCCATGGAGTAATGGCCGAACGCGCCGCGCGGGTGCGCGCCGAACCTTGGCAGCCTGGcgt > 4:222906/1‑133 (MQ=255) aaaGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATACTGCCGCGCTGCAGGATAATGCAATCGACATTCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCAAACCTTGGCAGCCTGGCTTTtccgt > 8:287903/1‑136 (MQ=255) aaGACGGGCGCTAGTCGAAAGCTGCATGTGGATGGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 7:121428/136‑1 (MQ=255) aaGACGGGCGCAAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 7:316257/136‑1 (MQ=255) agccGGGCGCTAGGCGAAAGCTGCATGTGGATGGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 1:34253/132‑1 (MQ=255) aGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 2:271626/135‑1 (MQ=255) cGGGCGCTAGTCGAAAGCTGCATGTGGATTTCCGGGTACTGCCGCGCGGCCGGAGCAGGGCATAGCCCTGGAGGTAAGGCCTAAACCCCCGCGCGGGGGAGCGCCGAAACATTGCCCCCTGGCGTTTACGGCCGc > 6:332584/1‑135 (MQ=255) cGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCGGATGCTGCCGCCCTGCCGGCTACCGCAATCGCCATTCACTAATGGCCGAACGCCCCGCCCCGGTGTGCGCCGAACCGTTGCAGCCGGGCGTTtccgtgccc > 4:334010/1‑131 (MQ=255) tCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCAACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCa < 3:18452/133‑1 (MQ=255) cGAAAGCTGAATGTGGATTGCCTGATCCTGCCGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAgg < 5:319458/135‑1 (MQ=255) cgctgctTGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGAAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGgaga < 4:374774/133‑1 (MQ=255) aaTGTGGATTGCCTGATCGTGCCGGTATTCATTTTCAGCAAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc < 6:337446/134‑1 (MQ=255) gtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAAt < 4:346043/135‑1 (MQ=255) gttCCTGCCGCTGTGATGTATAAGTCAATCGACATGCAGTAATTGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaac < 3:316327/133‑1 (MQ=255) gagccTGCCGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaac < 3:222906/132‑1 (MQ=255) cgcgCTGCTGGATCAGGAAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTc < 5:332584/135‑1 (MQ=255) cgcTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcac > 2:316745/1‑135 (MQ=255) cTGCAGGATCCGTCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGAAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccc < 5:68282/135‑1 (MQ=255) | AGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCC > ACB122/1584912‑1585176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |