| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,687,834 | C→G | 5.5% | G248G (GGC→GGG) | wspC → | putative biofilm formation methyltransferaseWspC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,687,834 | 0 | C | G | 5.5% | 272.0 / 6.3 | 88 | G248G (GGC→GGG) | wspC | putative biofilm formation methyltransferaseWspC |
| Reads supporting (aligned to +/- strand): ref base C (41/39); new base G (3/2); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01 | |||||||||||
GTTCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCAGCCC > ACB122/1687699‑1687969 | gtTCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGc > 7:19776/1‑136 (MQ=255) ttCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGc < 8:267719/135‑1 (MQ=255) ttCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGc < 7:135501/135‑1 (MQ=255) tCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCa > 3:273249/1‑135 (MQ=255) tCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCa > 5:325662/1‑135 (MQ=255) tCTGTCGCAACCTGTTGATCCATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCa > 5:193316/1‑135 (MQ=255) tGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATg < 3:107413/135‑1 (MQ=255) cGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATg < 4:89191/132‑1 (MQ=255) gCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCATGCTGGCACGCATGGGCATGCGTc > 2:101876/1‑135 (MQ=255) cTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTgg < 8:203126/136‑1 (MQ=255) tGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTTCTGTTCATCGGCCCGGCCGAAGGCAGCTTGGTGGCACGCATGGGCATGCGTCCATTgg < 1:136360/135‑1 (MQ=255) tGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTGGCTGGCACGCATGGGCATGCGTCCATTgg < 3:79362/135‑1 (MQ=255) aTCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATc > 6:49001/1‑135 (MQ=255) tCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATc < 6:127300/134‑1 (MQ=255) tttCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCa > 6:178202/1‑135 (MQ=255) cGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGt < 3:325613/134‑1 (MQ=255) cGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTc > 3:183857/1‑135 (MQ=255) aCGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGtt > 7:23441/1‑136 (MQ=255) tACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGTACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGc > 7:303471/1‑136 (MQ=255) aCCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGt < 6:306399/124‑1 (MQ=255) aCCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGt > 5:306399/1‑124 (MQ=255) aCCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTTCCTAt > 6:320025/1‑135 (MQ=255) gCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTTCCTATGTGCGTCa > 6:6277/1‑135 (MQ=255) gCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTTCCTATGTGAGTCa > 2:268400/1‑135 (MQ=255) cGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCAc < 6:193316/135‑1 (MQ=255) gtgtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGaa < 4:10667/135‑1 (MQ=255) tgtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTcc < 2:368141/94‑1 (MQ=255) tgtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTcc > 1:368141/1‑94 (MQ=255) tgtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGCAg > 1:116450/1‑135 (MQ=255) gtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTTCCTATTTGCGTCACGAAgg > 5:334712/1‑135 (MQ=255) gtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAgg > 5:28006/1‑135 (MQ=255) gtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAgg < 6:28006/135‑1 (MQ=255) gtTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGc > 7:171431/1‑136 (MQ=255) gAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCg > 4:53544/1‑133 (MQ=255) gTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAgc < 2:265312/135‑1 (MQ=255) tGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACa > 2:350477/1‑132 (MQ=255) tGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACa < 1:350477/132‑1 (MQ=255) gctgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTggcggc > 6:174515/1‑135 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGTGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAgg > 4:218110/1‑112 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGTGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAgg < 3:218110/112‑1 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGt < 7:193671/87‑1 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGt > 8:193671/1‑87 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGCCAGCGCTCCCTTggcggcgg > 5:142143/1‑135 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACCGCGCTCCCTTggcggcggc > 8:196613/1‑136 (MQ=255) tgcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGAGCTCCCGTggcggcggc > 7:131137/1‑136 (MQ=255) gcATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCTCCCAGTCGTTTGCCTAGGTGCGTCACGAAGGCGACAGCGCTCCCTTggcggcggc < 2:171220/135‑1 (MQ=255) aTCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGGCTATGTGCGTCACGAAGGCGACACCGCGCCCTTGGCGGCGGCAc > 6:397500/1‑135 (MQ=255) tCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCAGGCGTCCATTGGGGAGCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCAcc < 6:256011/135‑1 (MQ=255) cGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGc > 8:98132/1‑136 (MQ=255) gCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGAATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGcc < 8:275362/136‑1 (MQ=255) cAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGcc < 3:153361/135‑1 (MQ=255) cAGGGTGTGCTGATCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGAGCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGcc < 2:291483/135‑1 (MQ=255) tgtgCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACt < 8:17407/136‑1 (MQ=255) gCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCGATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGc > 6:51666/1‑135 (MQ=255) tGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGGATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTcc < 4:105421/106‑1 (MQ=255) tGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGGATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTcc > 3:105421/1‑106 (MQ=255) tGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGGATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTcc > 3:99778/1‑106 (MQ=255) tGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGGATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTcc < 4:99778/106‑1 (MQ=255) tGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCCCGAAGGCGACAGCGCTCCCGTGGCGGCGGCCCCCGCCCCAACTGCCaa > 8:361102/1‑136 (MQ=255) ttCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGAGCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAg < 7:398328/135‑1 (MQ=255) ttCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGGCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAg < 7:395925/135‑1 (MQ=255) tCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAgcgtg > 1:293421/1‑135 (MQ=255) gcccggccGCAGGACGCTTGCTGGCACGCATGGGAATGCGTCCATTGGGGAGCGCCCCGTCGTGTGCCTATGTGCGTCACGCCGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAgcgtgcgt < 2:218162/135‑1 (MQ=255) gcccggccGAAGGCAGCTTGCTGGCACGCTTGGGCAGGCGTCAATTGGGTAGCGCCATGTCGTTTGCTTTTGTGCGTCACTAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAgcgtgcgt < 1:201176/135‑1 (MQ=255) cggccGAAGGCAGCTTGCTGGTACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCac < 8:303471/136‑1 (MQ=255) gccGAAGGCAGCTTGCTGGCACGCAGGGGCTTGCGTCCATTGGGGAGCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCacg < 2:63884/135‑1 (MQ=255) ccGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGGCCCGAAGGCGACCGCGCTCCCTTGGCGGCGGCCCCCGCCCCACCTGCCAAGCGTGCGGTCacga > 2:304874/1‑135 (MQ=255) ccGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTGTTCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTTGCGGCGGCACCCGCCCCAACTGCCCAGCGTGCGTTGacgc > 5:92139/1‑134 (MQ=255) gAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCacgacg > 5:145550/1‑135 (MQ=255) cAGCTTGCTGTCACGCATGGGAATGCGTCCATTGGGTATCGCCCAGTCGTTTGACTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc < 7:91131/136‑1 (MQ=255) cAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGTGCGCCGAGTCGTTTGCCTATGTGGGTCCAGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc < 7:378913/136‑1 (MQ=255) tgctggctgGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGGGCGTTCACGACGTTGccc < 4:32125/134‑1 (MQ=255) aGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGCAGGCGCCAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc > 1:289143/1‑135 (MQ=255) aGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc < 3:150622/135‑1 (MQ=255) aGCTTGCTGGCACGAATGGGCATGCGTCCATTGGGGAGCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc < 1:357084/135‑1 (MQ=255) gCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGc < 7:98132/136‑1 (MQ=255) cTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGc < 4:317500/135‑1 (MQ=255) gcgggCACGCATGGGAATGCGTCCATTGGGGATCGCCCATTCGTTTGCCTTTGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCAcc < 6:23686/132‑1 (MQ=255) gCTGGCACGCATGGGGATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCCCCCGCCCCAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCg > 8:279322/1‑136 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGCCAGCGCTCCCTTGGCGGCGGCCCCCGCCCCAACAGCCAAGCGTGCGTTCACGACGTTGCCCGCAcc > 5:201447/1‑135 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCCCCCGCCCCAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCg > 8:117220/1‑136 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCCCCCGCCCAAACTGCCAAGCGTGCGTTCACGCCGTTGCCCGCAcc > 3:322035/1‑135 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc > 7:225587/1‑130 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGccc < 8:225587/130‑1 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACAGCCAAGCGTGCGTTCACGACGTTGCCCGCAcc > 4:374323/1‑135 (MQ=255) gCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTGTGGCTTTGTGCGTCACGAAGGCGGCCGCGCTCCCTTGGCGGCGGCCCCCGCCCCAACTGCCAAGCGTGCGTTGCCGCCGTTGCCCGCCCCg > 7:158690/1‑136 (MQ=255) cGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCAATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTAt < 3:387881/135‑1 (MQ=255) gCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATcc < 8:32500/136‑1 (MQ=255) gggCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCa < 4:183857/135‑1 (MQ=255) gggCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCa < 2:134117/135‑1 (MQ=255) gCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCAGc < 6:397199/135‑1 (MQ=255) cATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCAGccc < 7:273798/136‑1 (MQ=255) | GTTCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTCGAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGCAGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTGCGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCGTTCACGACGTTGCCCGCACCGGTTTATCCGCAGCCC > ACB122/1687699‑1687969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |