Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,791,678 T→G 32.8% L182R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6780TG32.8% 49.6 / 5.0 45L182R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (23/6);  new base G (6/10);  total (29/16)
Fisher's exact test for biased strand distribution p-value = 8.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGC  >  ACB122/1791545‑1791812
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gACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACCCCATACCGCTTGAAGCCGACCGCCCGGCCGCGCCAGCCGCGctg                                                                                                                                       >  4:240325/1‑135 (MQ=255)
gACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGctg                                                                                                                                       >  2:235021/1‑135 (MQ=255)
               ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCCCCCGCTGGCAGACACCATACCGCGTGAAGCCGCCCGCCCGGCCGCGCGGGGCGCGCGGGGCCGGGGCgggggg                                                                                                                        >  1:103733/1‑135 (MQ=255)
                             ggCGCTGTTCTCCGATGAGCAGGGGGTGCCAGTGCGGTTTGGCGGCCACCCGCTGGCCGACACCATACCGCGTGAAGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTg                                                                                                         <  8:140050/136‑1 (MQ=255)
                                               gCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGGGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGt                                                                                       >  8:346601/1‑136 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCCCCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGCGGGGGCCGTGGGGGCGGTGGAGCCCGGCCGACgggggg                                                                                      >  5:264842/1‑133 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCCGGCAGCCgggggg                                                                                      >  5:89910/1‑133 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAggg                                                                           >  7:295638/1‑136 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                            >  4:382836/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                            >  3:342850/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                            >  3:292280/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCCCCGGGGGCCTGGGGGGGGGGCCGGGGGTGGGGTTGTTGCCGGCCCCCCGGGGGGGCGCATTAgg                                                                            >  5:12616/1‑135 (MQ=255)
                                                                gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                       >  1:117799/1‑135 (MQ=255)
                                                                gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                       >  1:39992/1‑135 (MQ=255)
                                                                     ggcGGCCACCCGGTGGCAGACACCATACGGATTGAAGCCGACGGCCCGGCGGCGCGGGCGGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                  <  7:189772/133‑1 (MQ=255)
                                                                      gcGGCCACCCGCTGCCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                 <  6:328799/134‑1 (MQ=255)
                                                                       cGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcgc                                                                <  1:19852/135‑1 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGCTGCCGGGCCGCCGGGGGGGCGCAGGAGGGCGCTTGGGCGCGCTGTTCCTCGCCGCCGCCGCGCGcc                                      >  5:110932/1‑135 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                                      >  5:88263/1‑135 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGGGCTGTTCCTCGACGCCGCCGAGCGcc                                      >  1:2600/1‑135 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                     >  8:97821/1‑136 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCCCTGGGCCTGGGCGAGGGGCCCGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTACGGCGCTTGGGCGCGCTGTTCCTCGCCGCCGCCGCGCCCCt                                     >  8:345507/1‑136 (MQ=255)
                                                                                                 cGCTTGAAGCCGACCGCCCGGCCGCGCGGGGCCCGCGGGGGCGGGGGGCGGGGGCGGGGGTGGGGGTGTTGGCGGGGCGCCGGGGGGGCGGAGGTGGGCGCGTGGGCGGGCGGTTTCTCGCCCCCGCCCCGCGcc                                      >  6:245675/1‑135 (MQ=255)
                                                                                                    tggacgCCGACGGCCCGGCGGCGGGTGCCGGGCGGGGCGTGGGGGAGGGGCCGGGGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCa                                   <  3:138721/130‑1 (MQ=255)
                                                                                                      gacgccgccCGCCCGGCCGCGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGGGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                 <  7:15519/132‑1 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                 >  8:229311/1‑135 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCCGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTACGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCCGcc                                >  7:352862/1‑136 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                  >  2:299334/1‑134 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                 >  8:49973/1‑135 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCCGTGGTGGCGTTGATGCCGGGCAGCCGGGGTTGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                 >  7:57803/1‑135 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCAGGGCGGCGGGCCGGTGGTGGCGGTGGTGCCGGGCCGCCGGGGTGGGGAAGTAGGGCGCGTGGGCGCGCGGGTCCGAGCCGCCGCCGCGCGccgccgc                                 >  4:360973/1‑130 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGGGGGGGGGCGGGGGTGGGGGTGCTGCCGGGCCGCCCGGGGGGCGAAGTAGGGCGCGTGGGCGCGCTGTTGCTCGACGCCCCCGCGCGCCGCAGc                                 >  6:268808/1‑135 (MQ=255)
                                                                                                       aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGAAGTAGGGCCCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTccccc                                >  8:61084/1‑131 (MQ=255)
                                                                                                       aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                 >  4:25914/1‑134 (MQ=255)
                                                                                                        aGCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                               <  3:21320/135‑1 (MQ=255)
                                                                                                             cccGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                          <  6:105963/134‑1 (MQ=255)
                                                                                                                gCCCGGCCGCGCGTGGCGCGCGGGGCCTGGGCGGGGGGCCGGTGGTGGGGGTGCTGCCGGGCAGCCGGGGGGGCGAAGTAGGGCGCTTGGGCGCGCTGGTCCGCGCCGCCGCCGCGCCCCTCCGCCAACCAGTGc                       >  4:18295/1‑135 (MQ=255)
                                                                                                                gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGTTGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGCCGCCGCCGCGCGCCTCCGCCAACCAGTGc                       >  4:25921/1‑135 (MQ=255)
                                                                                                                gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGGTGGCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCGGTTCCTCGCCGCCGCCGAGCGCCTCAGCCAAGAAGTGc                       >  1:113966/1‑135 (MQ=255)
                                                                                                                  ccggccggGGGGGCCGGGGGGGGCCGGGGCGCGGGGCCGGTGGGGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCgg                    <  7:375219/136‑1 (MQ=255)
                                                                                                                   cGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCgg                    <  4:342850/135‑1 (MQ=255)
                                                                                                                      cggcgcgTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGTCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGgtgt                <  8:168916/134‑1 (MQ=255)
                                                                                                                        gggcgGGCCGCGCTGGGCGGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc              <  8:214803/134‑1 (MQ=255)
                                                                                                                         cgggTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc              <  5:331243/132‑1 (MQ=255)
                                                                                                                         cgcgTGCCGCGCTGGGACTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc              <  3:111511/135‑1 (MQ=255)
                                                                                                                         cgcgGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc              <  6:270566/135‑1 (MQ=255)
                                                                                                                              gCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTc         <  6:366093/135‑1 (MQ=255)
                                                                                                                                    gtgggcgtgggcTCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGGGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg   <  3:99981/134‑1 (MQ=255)
                                                                                                                                     tgggcctgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGc  >  6:198748/1‑135 (MQ=255)
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GACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTGC  >  ACB122/1791545‑1791812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑