Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,828,161 G→T 7.3% R152R (CGG→AGG)  sprT ← Protein SprT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,828,1610GT 7.3% 193.6 / 4.8 67R152R (CGG→AGG) sprTProtein SprT
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base T (3/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTGTGCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTG  >  ACB122/1828032‑1828296
                                                                                                                                 |                                                                                                                                       
tttGTGCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGTGCAGCGCACGTCTCGGCAATTCTACGCCCCCCGATCTATTCGACCCGCGACTCGCCGCTGTACACCAGGACTTCACGCCACCg                                                                                                                                   >  8:163294/1‑136 (MQ=38)
tttGTGCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCg                                                                                                                                   >  8:163292/1‑136 (MQ=255)
tttGTGCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCg                                                                                                                                   >  7:115658/1‑136 (MQ=255)
    tgCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCt                                                                                                                                 <  5:47016/134‑1 (MQ=255)
            cTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCCGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCTCCGCTGTACACCAGGACTTCCCTGCACCGCTTGCACAgtt                                                                                                                        >  1:184526/1‑133 (MQ=255)
            cTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACCCCAGGACTTCCCGGCACCGCTTGCACAGGt                                                                                                                        >  4:374569/1‑135 (MQ=255)
            cTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGt                                                                                                                        >  1:20225/1‑135 (MQ=255)
            cTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCAGATCTATTCGACCCGCGACTCGCCGCTGTACACCAGGACTTCCCGTCACCGCTTGCACAgct                                                                                                                        >  8:258530/1‑133 (MQ=255)
                      ccTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGccc                                                                                                              <  4:34645/135‑1 (MQ=255)
                           cgcgGGTAAACCCGCTCCTACGCGGGGCTGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         <  7:356379/135‑1 (MQ=255)
                            gctggTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         <  5:177136/131‑1 (MQ=255)
                            gcgGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCACGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTg                                                                                                        >  5:111900/1‑135 (MQ=255)
                                            cTACGCGGGGCAGCGCACGTCTCGGGACTTGCACGACCCCCGATCTATTCGACCCGCGTCTCGACGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGCGCCGCt                                                                                       <  7:163294/136‑1 (MQ=255)
                                            cTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCt                                                                                       <  7:163292/136‑1 (MQ=255)
                                            cTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCt                                                                                       >  7:273269/1‑136 (MQ=255)
                                             tACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCt                                                                                       <  5:94672/135‑1 (MQ=255)
                                             tACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCt                                                                                       >  6:389653/1‑135 (MQ=255)
                                                 cgGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTgcgc                                                                                   >  3:141897/1‑135 (MQ=255)
                                                       aGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGGCGCGGCGGGGTaaa                                                                             >  6:44171/1‑135 (MQ=255)
                                                       aGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCAGCCTCTCGCCGCTGTTCACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGGCCCGGCGCgggtca                                                                             >  5:238761/1‑131 (MQ=255)
                                                         cgcACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTAGTGCCGCTGCGCGGTaaaag                                                                           >  2:298459/1‑133 (MQ=255)
                                                            aCGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGa                                                                        <  5:389653/135‑1 (MQ=255)
                                                                 tcGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCg                                                                   >  5:32378/1‑135 (MQ=255)
                                                                 tcGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTACCGGCACCGATTGCACAGGTATCGCCGCCATTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCg                                                                   >  1:62898/1‑135 (MQ=255)
                                                                      aaTTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGAATCGCCGCCCTTGGTGAACCAGCTTGTGCCGCTGCGCGGTTAACGGGAAGTCGCTTTg                                                              >  6:291877/1‑135 (MQ=255)
                                                                             cGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcac                                                       <  3:48275/135‑1 (MQ=255)
                                                                             cGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcac                                                       <  8:273269/135‑1 (MQ=255)
                                                                              gACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGTACTTCCCGGCACCGCTTGCTCAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcacgg                                                     <  7:258530/136‑1 (MQ=255)
                                                                              gACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcacg                                                      <  5:375238/135‑1 (MQ=255)
                                                                               aCCCCCGATCTATTCGACCCGCTTCTCGCCGGTTTACACCAGGACTTCCCGGCACCGCTTGAACAGGTTTCGCGGCCCTTGGTGCACCAGATTGTGCCGCTGCGCGGTAAAGGGGAAGTCGCTTTGcgggcacgg                                                     <  2:14172/135‑1 (MQ=255)
                                                                                  cccGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGcgggcacgggca                                                  <  3:385393/135‑1 (MQ=255)
                                                                                   ccGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAg                                                 >  1:261031/1‑135 (MQ=255)
                                                                                   ccGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGc                                                >  7:113684/1‑136 (MQ=255)
                                                                                        cTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCg                                               >  3:234983/1‑132 (MQ=255)
                                                                                        cTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCAGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCg                                               >  2:17821/1‑132 (MQ=255)
                                                                                           ttCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCgg                                              <  8:113684/130‑1 (MQ=255)
                                                                                            tCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCgg                                              <  2:20225/129‑1 (MQ=255)
                                                                                            tCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGAt                                         <  6:111900/134‑1 (MQ=255)
                                                                                            tCGACCCGCGTCTCGCCGCTGGACACCAGGACTTACCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCgg                                              <  3:35159/129‑1 (MQ=255)
                                                                                             cGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGAt                                         >  6:185672/1‑133 (MQ=255)
                                                                                             cGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGCAAGTCGCTTTTCGGGCACGCGCAGCGGTAGATg                                        >  8:223524/1‑134 (MQ=255)
                                                                                              gACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCt                                                                 <  8:393871/108‑1 (MQ=255)
                                                                                              gACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCt                                                                 >  7:393871/1‑108 (MQ=255)
                                                                                              gACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTa                                      >  2:122248/1‑135 (MQ=255)
                                                                                              gACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCGTTGCGGGCACGGGCAGCGGTAGATGTa                                      >  5:191212/1‑135 (MQ=255)
                                                                                              gACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGGAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGGAGATGTa                                      >  1:25602/1‑135 (MQ=255)
                                                                                                   gcgTCTCGCCGCTGTACACCAGGACTTCCCTGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         <  3:249294/63‑1 (MQ=255)
                                                                                                   gcgTCTCGCCGCTGTACACCAGGACTTCCCTGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         <  3:254038/63‑1 (MQ=255)
                                                                                                   gcgTCTCGCCGCTGTACACCAGGACTTCCCTGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         >  4:249294/1‑63 (MQ=255)
                                                                                                   gcgTCTCGCCGCTGTACACCAGGACTTCCCTGCACCGCTTGCACAGGTATCGCCGCCCTTGGt                                                                                                         >  4:254038/1‑63 (MQ=255)
                                                                                                      tctcGCCGCTGTACACCAGGACTTCCCGGCACCGCTAGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTTTGCCGCTGAGCGGTCAACGGGAAGTCGCTTTGCGCGCCCGGGCAGCGGGAGATGTAGCGGGTCa                              >  6:171080/1‑135 (MQ=255)
                                                                                                        tcGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACt                            >  6:74951/1‑135 (MQ=255)
                                                                                                           ccgcTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACt                            <  3:112848/132‑1 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCAGCGCTTGCACAGGTATCGCCGCCCTTGGTGCACGAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCg                    <  1:298459/133‑1 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCg                    <  5:185672/133‑1 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGtt                  <  6:32378/135‑1 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGtt                  <  5:44171/135‑1 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGtt                  >  4:383701/1‑135 (MQ=255)
                                                                                                                  acacCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGtt                  <  4:234983/135‑1 (MQ=255)
                                                                                                                       aGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACg             <  2:25240/135‑1 (MQ=255)
                                                                                                                         gACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAg                                                 >  1:334674/1‑97 (MQ=255)
                                                                                                                         gACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAg                                                 <  2:334674/97‑1 (MQ=255)
                                                                                                                            ttCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTgcgc                                                                                   <  3:124808/60‑1 (MQ=255)
                                                                                                                            ttCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTgcgc                                                                                   >  4:124808/1‑60 (MQ=255)
                                                                                                                            ttCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATa        <  1:385320/135‑1 (MQ=255)
                                                                                                                              cccGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCGCGTCATAGt      >  3:243018/1‑135 (MQ=255)
                                                                                                                              cccAGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCCCTGCGCGGGAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTATCGGGTAACTTCGCGCCGGTGCCCGTCATAGt      >  1:131120/1‑135 (MQ=255)
                                                                                                                                 ggCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTtgtg  >  8:270971/1‑136 (MQ=255)
                                                                                                                                 ggCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTtgt   >  5:86648/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                       
TTTGTGCATTGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGCGGGGCAGCGCACGTCTCGGGAATTGTACGACCCCCGATCTATTCGACCCGCGTCTCGCCGCTGTACACCAGGACTTCCCGGCACCGCTTGCACAGGTATCGCCGCCCTTGGTGCACCAGCTTGTGCCGCTGCGCGGTAAACGGGAAGTCGCTTTGCGGGCACGGGCAGCGGTAGATGTAGCGGGTCACTGCGCGCCGTTGCACGTCATAGTTGTG  >  ACB122/1828032‑1828296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑