Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,856,481	G→A	5.4%	G11S (GGC→AGC)	CJ019_01772 →	Sodium‑lithium/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,856,481	0	G	A	5.4%	245.6 / 2.2	74	G11S (GGC→AGC)	CJ019_01772	Sodium‑lithium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (21/49);  new base A (2/2);  total (23/51)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTCGCGTACCT  >  ACB122/1856350‑1856616                                                                                                                                    |                                                                                                                                        cAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGgcg                                                                                                                                      <  3:50734/135‑1 (MQ=255) cAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTGTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCGGGATCTGGCTGGgcg                                                                                                                                      <  6:353360/135‑1 (MQ=255)  aGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGgcgc                                                                                                                                     >  2:37845/1‑135 (MQ=255)  aGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGgcgc                                                                                                                                     >  1:236984/1‑135 (MQ=255)  aGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCt                                                                                                                                    <  7:299487/136‑1 (MQ=255)   gTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCt                                                                                                                                    <  3:145222/135‑1 (MQ=255)   gTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCt                                                                                                                                    <  2:368284/135‑1 (MQ=255)   gTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCt                                                                                                                                    <  2:227800/135‑1 (MQ=255)   gTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCt                                                                                                                                    <  6:331464/135‑1 (MQ=255)       cGGCAGTGGCCGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCGGGATCTGGCTGAGCGCTGCAc                                                                                                                                >  3:84794/1‑135 (MQ=255)       cGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCAct                                                                                                                               <  7:208653/136‑1 (MQ=255)       cGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCAct                                                                                                                               <  7:20436/136‑1 (MQ=255)       cGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGAGCGCTGCAc                                                                                                                                >  3:90307/1‑135 (MQ=255)           aggggACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCACGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCActgct                                                                                                                            <  5:326379/132‑1 (MQ=255)            gTGGACGCCGCCGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGCCTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCActgct                                                                                                                            >  7:390149/1‑134 (MQ=255)            gTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCActgctg                                                                                                                           >  3:391233/1‑135 (MQ=255)             tGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTTACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGa                                                                                                                          <  1:79355/135‑1 (MQ=255)                 cgccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGCCGGCTGGCGGTTTCGCTGGTAAGGAGCGCGTGCCGTGCCTGACATGCGTCGCTGGCTCTGGCTGGGCGCTGCACTGCTGATCGc                                                                                                                      >  6:396231/1‑135 (MQ=255)                 cgccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGTCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  8:136845/136‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCCAGCCGTTCGACGGCTTGCGGTGTCGCTGGTAAGGAGCGCTTGCCGTGTCTGACATGCGTCGCTGGATCTGGCGGGGCGCTGCACTGCTGATCGCt                                                                                                                     >  1:225867/1‑135 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  6:104232/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  5:232078/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  5:220443/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  5:199994/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  6:48493/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  6:63520/135‑1 (MQ=255)                  gccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  1:77920/135‑1 (MQ=255)                   ccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCtgt                                                                                                                   <  8:374125/136‑1 (MQ=255)                   ccgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTAGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCtg                                                                                                                    <  6:257317/135‑1 (MQ=255)                    cgcAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCtgt                                                                                                                   <  2:176838/135‑1 (MQ=255)                       aGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACCGCTTGCGTTTTAGCAGGTAAGGAGCGCTTGCCGTGAATGACATGCGTCGCTGGATGTGGGTGGGCGCTGCTCTGCTGATCGCTGtgca                                                                                                                >  1:109886/1‑134 (MQ=255)                              cAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTtat                                                                                                         <  2:395256/135‑1 (MQ=255)                               aGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTtata                                                                                                        >  4:87686/1‑135 (MQ=255)                                  ccGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCt                                                                                                    <  7:6521/136‑1 (MQ=255)                                  ccGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCt                                                                                                    <  8:317580/136‑1 (MQ=255)                                   cgcgCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCt                                                                                                    <  1:280656/135‑1 (MQ=255)                                     cgcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGca                                                                                                 <  7:15381/136‑1 (MQ=255)                                     cgcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGca                                                                                                 <  7:213774/136‑1 (MQ=255)                                      gcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGca                                                                                                 <  1:174401/135‑1 (MQ=255)                                      gcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGca                                                                                                 <  1:336336/135‑1 (MQ=255)                                      gcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGca                                                                                                 <  3:11272/135‑1 (MQ=255)                                      gcCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGc                                                                                                  >  5:369555/1‑134 (MQ=255)                                        cTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGcaca                                                                                               >  3:390088/1‑135 (MQ=255)                                              agccagcACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     >  8:390681/1‑107 (MQ=255)                                              agccagcACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCt                                                                                                                     <  7:390681/107‑1 (MQ=255)                                                  agcACCCAAGCCGTTCGACGGCTTGCGGTTTCGCTGGGAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTAc                                                                                     <  4:18553/135‑1 (MQ=255)                                                        cAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTc                                                                              >  8:136123/1‑136 (MQ=255)                                                          aGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCt                                                                            <  8:286258/136‑1 (MQ=255)                                                          aGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCt                                                                            >  8:182502/1‑136 (MQ=255)                                                          aGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCt                                                                            <  7:42495/136‑1 (MQ=255)                                                           gCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCt                                                                            <  1:61966/135‑1 (MQ=255)                                                           gCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCt                                                                            <  6:369555/135‑1 (MQ=255)                                                             cGTTCGACGGCTTGCGTTTTCGCTGGTAAGGCGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTGTATAGGCTGCACAATATTCTTACGCCGTTCCTGGt                                                                         >  7:169308/1‑136 (MQ=255)                                                             cGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTgg                                                                          >  3:122191/1‑135 (MQ=255)                                                                tCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCg                                                                       <  4:301906/135‑1 (MQ=255)                                                                tCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCg                                                                       <  1:292396/135‑1 (MQ=255)                                                                tCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCg                                                                       <  6:367648/135‑1 (MQ=255)                                                                  gACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGAGCGCTGTGCTGCTTGATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGc                                                                     >  6:178799/1‑135 (MQ=255)                                                                    cGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGcatc                                                                  <  7:196424/136‑1 (MQ=255)                                                                     ggCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGcatc                                                                  <  1:280072/135‑1 (MQ=255)                                                                           cGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGc                                                              <  6:36059/133‑1 (MQ=255)                                                                                       tAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGc                                                 <  2:231587/134‑1 (MQ=255)                                                                                       tAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGc                                                 <  2:109886/134‑1 (MQ=255)                                                                                                   gCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGTCATCATGCTGGCCTACCTGGCCGACCCGCTGGTc                                    >  8:343862/1‑135 (MQ=255)                                                                                                   gCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGt                                     >  3:180487/1‑134 (MQ=255)                                                                                                   gCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTc                                    >  7:119006/1‑135 (MQ=255)                                                                                                   gCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTc                                    >  6:247636/1‑135 (MQ=255)                                                                                                   gCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGCGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGt                                     >  2:113319/1‑134 (MQ=255)                                                                                                          ctgacATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGtct                            <  8:266197/136‑1 (MQ=255)                                                                                                          ctgacATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGt                              <  1:374454/134‑1 (MQ=255)                                                                                                           tgacATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGtct                            <  2:149997/135‑1 (MQ=255)                                                                                                           tgacATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGtct                            <  4:175472/135‑1 (MQ=255)                                                                                                                       cTGGATCTGGCTGAGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTggg                <  4:90307/135‑1 (MQ=255)                                                                                                                       cTGGATCTGGCTGAGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTggg                <  4:84794/135‑1 (MQ=255)                                                                                                                                 ctggGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTcgcg       <  5:122445/134‑1 (MQ=255)                                                                                                                                 ctggGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTc          <  4:218443/131‑1 (MQ=255)                                                                                                                                  tggGCGCTGCACTGCTGATCGCTGTGCTGCTTtat                                                                                                         <  5:262372/35‑1 (MQ=255)                                                                                                                                  tggGCGCTGCACTGCTGATCGCTGTGCTGCTTtat                                                                                                         >  6:262372/1‑35 (MQ=255)                                                                                                                                  tggGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTCGCGTa     >  2:385982/1‑135 (MQ=255)                                                                                                                                    ggCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTCGCGTAc    <  7:182502/134‑1 (MQ=255)                                                                                                                                    ggCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTCGCGTACCt  >  8:13856/1‑136 (MQ=255)                                                                                                                                    |                                                                                                                                        CAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCGCTTGCCGTGACTGACATGCGTCGCTGGATCTGGCTGGGCGCTGCACTGCTGATCGCTGTGCTGCTTTATAGCCTGCACAATATTCTTACGCCGTTCCTGGTCGGCATCATGCTGGCCTACCTGGCCGACCCGCTGGTCGATCGTCTCGAGCGCCTGGGGCTTTCGCGTACCT  >  ACB122/1856350‑1856616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑