Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122179,4840TC100.0% 244.8 / ‑6.8 70C316R (TGC→CGC) CJ019_00182hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (22/47);  minor base G (0/1);  total (22/48)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB122/179360‑179614
                                                                                                                             |                                                                                                                                  
tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          >  3:209946/1‑135 (MQ=255)
         ggctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  4:209946/135‑1 (MQ=255)
          gctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  7:304744/134‑1 (MQ=255)
           ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGCACAGATGCGTCGTACTTGTGCCAGCTACCCGGAACCCAACGACCACATTTCCCGCAGCCGCGGCGGCGCGtgag                                                                                                               >  1:193293/1‑132 (MQ=255)
           ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                >  1:273278/1‑134 (MQ=255)
           ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                >  3:339109/1‑134 (MQ=255)
           ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  4:339109/133‑1 (MQ=255)
           ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTACCGCAGCCGCGGCGGCGAGttt                                                                                                                >  2:210001/1‑134 (MQ=255)
               ccTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGt                                                                                                           <  2:309588/135‑1 (MQ=255)
                    tcgcccgcgTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTTAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  8:153069/134‑1 (MQ=255)
                    aCGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  6:75322/135‑1 (MQ=255)
                      gcccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCTAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTCCCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCAc                                                                                                   >  7:310701/1‑136 (MQ=255)
                      gcccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCa                                                                                                    >  6:306182/1‑135 (MQ=255)
                         cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  7:334139/136‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTCCCGACGAGTGTTGCCAAGCGGTAGGTCAACCGCTGAGTCTTACTTGTGCCAGCTACCCGTAACCCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:193293/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCTGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGTAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:220676/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  1:217542/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  3:232923/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:216827/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:112237/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:61319/135‑1 (MQ=255)
                          gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGACACCgg                                                                                                <  4:210036/135‑1 (MQ=255)
                          gccTTGGAGGCCAGCGTTGCGACCAGTTGTTGCAAGCGGTAGGTGAAGAGCTGCGTCGTACTTGTGCCAGGTACCCGGTTACCTACGTCCTCATTTCCGGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCAGCggg                                                                                               <  8:205111/136‑1 (MQ=255)
                          gccGTGGCGGCCAGCGGACCGACCAGTTGTTGCAAGCGGTATGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCCACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  4:213340/135‑1 (MQ=255)
                           ccTTGGCGGCCAGCGTACCGACCAGTGGTTGCCAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGc                                                                                              <  8:368900/136‑1 (MQ=255)
                             ttGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCACCGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCa                                                                                             >  5:54053/1‑135 (MQ=255)
                                gcggcCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATg                                                                                           <  2:80444/134‑1 (MQ=255)
                                gcggcCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATg                                                                                           <  3:73809/134‑1 (MQ=255)
                                      aGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  8:236251/136‑1 (MQ=255)
                                       gCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCTGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  4:26600/135‑1 (MQ=255)
                                         gTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATgagg                                                                                 <  4:242458/135‑1 (MQ=255)
                                             cGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGaggagg                                                                              <  6:267927/134‑1 (MQ=255)
                                                 cAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGt                                                                          >  1:322840/1‑134 (MQ=255)
                                                  aGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGCCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTaca                                                                       >  7:267614/1‑136 (MQ=255)
                                                     tgttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCTCCGGGCATGGTGCATGAGGAGGCAGTACAcc                                                                     >  2:265474/1‑135 (MQ=255)
                                                       tggCAAGCGGGAGGGGATCAGCTGCGTCGTACTTGTGCCAGCGACCCGGAACCCCACGACCTCATTTCCCGCAGGCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCtt                                                                   <  6:264417/133‑1 (MQ=255)
                                                        tgCCAGCGCTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGCAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  5:398905/135‑1 (MQ=255)
                                                        tgCAAGCGGTTGGTGATCAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAAGGACCTCCTTTCGCGCAGCCGCGGCGGCGAGTTTGCATTGCTGTCTCCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  1:210001/135‑1 (MQ=255)
                                                        tgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCtt                                                                   <  5:79959/134‑1 (MQ=255)
                                                        tgCAAGCGGTAGGGGAACTGCTGCGGCGTACTGGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGTCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  5:38678/135‑1 (MQ=255)
                                                           cAAGCGGTAGGGGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGc                                                                 <  6:312943/134‑1 (MQ=255)
                                                                       gAACAGCTGCGTCGAACTTGTGCCAGCAACCCGGAACCCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAg                                                    <  4:54550/135‑1 (MQ=255)
                                                                         aCAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCCGGAgg                                                   <  6:357409/134‑1 (MQ=255)
                                                                             cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACCCCTTGCCCCGGCTCTGGAGGCAACg                                              >  1:198167/1‑135 (MQ=255)
                                                                             cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  >  5:44307/1‑115 (MQ=255)
                                                                             cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  6:44307/115‑1 (MQ=255)
                                                                             cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACg                                              >  6:384500/1‑135 (MQ=255)
                                                                                   cgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGc                                          >  6:387078/1‑133 (MQ=255)
                                                                                   cgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGc                                          >  3:60109/1‑133 (MQ=255)
                                                                                     tACTTGTGCAAGATACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTTCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCagag                                      <  3:345309/135‑1 (MQ=255)
                                                                                        ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTTCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  3:113074/135‑1 (MQ=255)
                                                                                        ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  8:271141/86‑1 (MQ=255)
                                                                                        ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    >  7:271141/1‑86 (MQ=255)
                                                                                          gtgCCAGCTAGCGGGAAACCAACGACCTCATTTCCCGCCGCCGCGGCGGGGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCTGGCTCTGGAGGCAACGTTGCAGAGCCTGc                                 <  7:255409/135‑1 (MQ=255)
                                                                                             ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                   >  7:269450/1‑114 (MQ=255)
                                                                                             ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                   <  8:269450/114‑1 (MQ=255)
                                                                                             ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCAc                               >  4:210000/1‑134 (MQ=255)
                                                                                                  tACCCGGAAACCAACGAACTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         <  6:377389/135‑1 (MQ=255)
                                                                                                   aCCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  6:27869/37‑1 (MQ=255)
                                                                                                   aCCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          >  5:27869/1‑37 (MQ=255)
                                                                                                                  aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      >  7:335736/1‑42 (MQ=255)
                                                                                                                  aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  8:335736/42‑1 (MQ=255)
                                                                                                                    cTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCTGTGCTGGCACCGGGAATGGTGGATGATGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCATCGTTGCAGTGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  3:322229/135‑1 (MQ=255)
                                                                                                                      cATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCg    >  7:223728/1‑136 (MQ=255)
                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCTTGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  2:236633/135‑1 (MQ=255)
                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCTTGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  6:54053/135‑1 (MQ=255)
                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  4:60109/135‑1 (MQ=255)
                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGAATGGTGCAGGAGGAGGCAGTACACCTTGCCCAGGCTCTGTAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  2:230491/135‑1 (MQ=255)
                                                                                                                          tCCCGCAGCCGCGGCGGCGATTTTGCAGTGCTGGCACCGGGCTGGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCTGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  2:82485/134‑1 (MQ=255)
                                                                                                                          tCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  5:306182/134‑1 (MQ=255)
                                                                                                                             |                                                                                                                                  
TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB122/179360‑179614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: