| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,892,407 | A→C | 29.6% | intergenic (+421/‑99) | PP_RS08755 → / → napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,892,407 | 0 | A | C | 29.6% | 39.2 / 3.5 | 37 | intergenic (+421/‑99) | PP_RS08755/napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base C (9/3); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGCTCCCACAGGTACTGCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTC > ACB122/1892275‑1892532 | cccGCCCCCACAGGAAGGCCCCCCCCCGCCGCCCTGGCGATAACCGTGGGGGCGGGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGc < 4:192053/135‑1 (MQ=255) caccGGTACTGCGCCGCCCGCAGGCCTTGCGATATCCCTGGGGGAGCGGGCAGGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTcacca < 2:61524/135‑1 (MQ=255) gTACTGCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCCACGTCGACCGCCCTCACCACTTCa > 2:55125/1‑135 (MQ=255) cTGCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTTCCTCATGCCGGGGGGTGGACCTCGGCCGCCCTCCCCACTTcgcg > 3:137376/1‑134 (MQ=37) gcgcCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCAGAAATTGCTGATCTACCTCATCCCGGGCGGTCAACGTCGCCCGCCCGCACCACTTCACGAc > 1:263979/1‑134 (MQ=39) gccgccCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCGGTCAACGTCGCCCGCCCGCCCCACTTCACGACaaa > 3:82582/1‑135 (MQ=39) gccgccCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGATGATCTACCTCATCCCCGGCGGTGCACGTCGACCGCCCGCACCCCAGCCCGACaaaa > 7:213059/1‑135 (MQ=37) cgccCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTCCCTCATCCCGGGCGGGCCACGGGGGCAGCCCGCCCCCCTTCCCGACaaacg > 1:162779/1‑133 (MQ=37) cgccCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGGGGTCAACGTCGCCCGCGCTCCCCCCGTCACCACaaacg > 6:135701/1‑133 (MQ=38) accAGGCCTTGCTAATTCCCTGTGGGAGCGGGCTTGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGcc < 4:73401/133‑1 (MQ=255) tCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGCTCTACCTCATCCCGGGCGGTGAACCTCGCCCGCCCGCCCCCCCTCACCACAAACGCGCCgt > 8:58861/1‑136 (MQ=37) tCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCCCCCCATCAAGACAAACccgccg > 6:287134/1‑135 (MQ=38) tCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGGCGCCAGCCCTCACCCCTTCACTCCAAACGCCCCg > 6:281953/1‑135 (MQ=38) ccTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTg > 3:26457/1‑135 (MQ=255) gATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTGACAGTGGTCAGCCCGAACCCCATCACGACAAAGGCGCCCGGTTGcacccc > 5:371266/1‑133 (MQ=37) tCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTGAACGGGGAGAGACCGCTCCACATCCCGACAAACGCGCCCAGGTGCCCCGCGTGc > 3:244072/1‑135 (MQ=25) ccTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCGGGCAACGTCGACAGCCCGCCCCACTTCACGACAAACGCGCCGTGGTGGCCCGcgttcct > 1:349108/1‑129 (MQ=38) ggCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTAAAGACAAAGGCGCCGTGTTGACCCGCTTGCATCAGCATTCCggg > 6:373015/1‑135 (MQ=255) gCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCCGGCTGTCAACGTCGACCGCCCTCACCACTTCACGACAAACGCGCCCTGTTGCCCCGCTTGCATCAGCATGCCgggg > 1:34722/1‑135 (MQ=255) gCCGGTAAAGCTGGCCAATAAATTGCTGATCTACCTCATCCCGGCCTGTCAACGTCGACAGCCCTCACCACTTCACGACAACGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCttttt < 3:26634/135‑1 (MQ=255) tataGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCAt < 3:345004/101‑1 (MQ=255) tataGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCAt > 4:345004/1‑101 (MQ=255) taGCTGGCCCATAAATTGCTGCTCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCACCCCTTCCCGACAAAGGCGCCGTGTTGCCCCGCGTGCATCCGCATGCCGGGGCA‑GCCGGAGGCTGTTTGCGgtt > 2:27959/1‑134 (MQ=255) aTAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAg > 7:94902/1‑66 (MQ=255) aTAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAg < 8:94902/66‑1 (MQ=255) tAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCCACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTGGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAgcc < 1:55125/135‑1 (MQ=255) tAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGGCGCCAGCCCTCACCCCATCACGACAAACGCGCCGTGTTGCCCCGCATGCATAAGCATGCCGGGGCA‑GCCGGCGCCATTTTGCGTTTTCCACAGGGgcc > 6:91841/1‑135 (MQ=255) aTTGCTGATCTACCTCATCCCGGGCTGTAAACGTAGACAGCCCTCACCACTTCACCACAAAAGCCCCGTGTTTCCCCCCCTGCAACAGCCTTCCCGGGCACCCCGCCGCCTTTTTAGGTTTACACAAGGAgcccc > 2:378189/1‑134 (MQ=255) tcaTCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCAt < 4:377383/132‑1 (MQ=255) cccTCATCCGGGGCGGTCAACGTCGACAGCCCTCACAACTTCACGACAACGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCGGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACa < 7:28227/135‑1 (MQ=255) tCACCCCGGGCTGGCAAGGCCGACACCCCTCACCACTACACTACAACGGCGCCGGGTGCCCCCCCTGGAATCACCTGGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGc < 1:324194/135‑1 (MQ=255) ccGGGCTGTCAACGTCGACCGCCCCCACCACTTCACGACAACGGCGCCGTGTGGCCCCGCTGGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTa < 8:218993/136‑1 (MQ=255) cGGGCTGTCAACGTCGACCGCCCTCCCCACATCACGACAAAGGCCCCGTGTTGCCCCGCATGCATCAGCATGACGGGGCA‑GCCCGCCCCGTTTTGCGTTTTAAACACGAGCCCGCCCCAGGCCAACACCGAAGAAc > 8:162322/1‑136 (MQ=255) cTGTCAACGTCGCCCTCCCTCCCCCCTTCACGACAACGGCGCCGGGTTGCCCCGCTTGCAGCAGAAGGCCGGGGCT‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCa < 3:292205/135‑1 (MQ=255) cTGCCACCGTCGCCCCCCCGCACCCCTTCCCGACAACGGCGCCGGGTGGCCCCGCTGGCATCACCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCa < 5:196996/135‑1 (MQ=255) gTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCGTTTTGCGGTTTGCACAGGCGCCCGCCCATGGCCAACCCCGAAGTACGCAGCg > 8:297674/1‑136 (MQ=255) aaCGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTc < 4:173483/135‑1 (MQ=255) | CCCGCTCCCACAGGTACTGCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCA‑GCCGGCGCCTTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTC > ACB122/1892275‑1892532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |