Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,037,270	C→G	5.6%	E57Q (GAA→CAA)	CJ019_01932 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,037,270	0	C	G	5.6%	211.7 / 5.9	71	E57Q (GAA→CAA)	CJ019_01932	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/42);  new base G (2/2);  total (27/44)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

CAGGTCGGCCTGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAACAGCAGCAACG  >  ACB122/2037142‑2037404                                                                                                                                 |                                                                                                                                       caggtcGGCCTGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCata                                                                                                                                 >  8:330140/1‑136 (MQ=255)  aggtcGGCCTGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCatat                                                                                                                                <  7:93053/136‑1 (MQ=255)   ggtcGGCCTGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCatat                                                                                                                                <  1:246480/135‑1 (MQ=255)     tcGGCCTGGCCGTCCTTGAGCGGCTGGTCATGCTGGTTCGCGTACATGCCGCTGGCTTCGGCCGGGTAAACAAGGCCTTGCCAGGCCGGCATATTGGTGCCGTAGGTAATGAATACGCCACCTGCGGGCAGATCtt                                                                                                                             >  7:265516/1‑136 (MQ=255)           tGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGtt                                                                                                                        >  2:249497/1‑135 (MQ=255)            ggCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGGCATCTTGTTGCCGTAGTTTATGAAAAAGCCACCGGCGGGCATATCTTCGGtt                                                                                                                        >  4:158188/1‑134 (MQ=255)                     tGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTAc                                                                                                              >  4:221416/1‑135 (MQ=255)                          ttCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCg                                                                                                         <  5:60865/135‑1 (MQ=255)                              ggTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGAt                                                                                                    >  7:110129/1‑136 (MQ=255)                              ggTCATGCTGTTTCGAGTACATGACGCTGGATTCGGCCTGGTAAACAAGGCCTTGCCTGGCGGTCATCTTGTTGCCGTATTTAATGAATAAGACACAGTCTGTCATATCTTCGGTTTCATTGTTACGGCAGTTGa                                                                                                     >  2:299424/1‑135 (MQ=255)                               gTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAATCCACCTTCGGGCATATCTTAGGTTTAATTGTTTCGGCCGTTGAt                                                                                                    >  4:254642/1‑135 (MQ=255)                               gTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGtgggt                                                                                                    >  1:2718/1‑131 (MQ=255)                               gTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGAt                                                                                                    >  1:269889/1‑135 (MQ=255)                               gTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGAt                                                                                                    >  4:31222/1‑135 (MQ=255)                                tCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCCCCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGtgggtt                                                                                                   >  3:233593/1‑130 (MQ=255)                                tCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTg                                                                                                      <  4:234559/132‑1 (MQ=255)                                  aTGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTc                                                                                                 <  8:25466/135‑1 (MQ=255)                                    gCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCa                                                                                               <  2:188996/135‑1 (MQ=255)                                      tGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGt                                                                                             >  3:250518/1‑135 (MQ=255)                                         ttCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTgg                                                                                          <  5:50964/135‑1 (MQ=255)                                            gAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGgtg                                                                                       <  8:179332/135‑1 (MQ=255)                                            gAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGgtg                                                                                       <  7:364315/135‑1 (MQ=255)                                             aGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGgtg                                                                                       <  2:387529/134‑1 (MQ=255)                                              gTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTa                                                                                     <  2:69518/135‑1 (MQ=255)                                              gTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTa                                                                                     <  2:47956/135‑1 (MQ=255)                                                    gCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTccc                                                                               <  6:259050/135‑1 (MQ=255)                                                    gCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTccc                                                                               <  4:331103/135‑1 (MQ=255)                                                     ccGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCtt                                                                             >  8:98349/1‑136 (MQ=255)                                                     ccGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCt                                                                              >  5:174916/1‑135 (MQ=255)                                                       gCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGt                                                                           >  8:303653/1‑136 (MQ=255)                                                        cTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTa                                                                          <  7:342327/136‑1 (MQ=255)                                                        cTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTa                                                                          <  7:393479/136‑1 (MQ=255)                                                         tGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAg                                                                         >  8:239938/1‑136 (MQ=255)                                                           gCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAgg                                                                        <  7:37906/135‑1 (MQ=255)                                                           gCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAgg                                                                        <  3:221416/135‑1 (MQ=255)                                                                ggCCTGGTAAACAAGGCCTTGCCTGGCGGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTGATTGTTACGGCCGTTGGTTTCAAGTTGGGTGTTGCTCCCGTGTAGGCCAAgc                                                                  >  8:343691/1‑135 (MQ=255)                                                                 gCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGTGTGTAGCTCCCTTGTAGGCCAAgg                                                                  <  3:208751/135‑1 (MQ=255)                                                                   cTGGTAAACAAGGCCTTGCCTGGCCGTGATCTTGTTTGCGTAGTTAATGAAAAAGCCACCTTCGGGCATATCTTCGGTTTAATTGTTTCGGCCGTTTAATTACAGTTGGGGGTAGCAACCGTGTTGGGCAAAGGt                                                                >  4:325456/1‑135 (MQ=255)                                                                         aaCAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGt                                                                <  3:94481/129‑1 (MQ=255)                                                                         aaCAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGt                                                                >  4:94481/1‑129 (MQ=255)                                                                          aCAAGGCCTTGCGTTGCCGTCATCTTGTTGCCGTAGTTAATTAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGCCCATGGGTGaacaa                                                          <  1:187464/134‑1 (MQ=255)                                                                          aCAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCTGGAATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGaacaa                                                          <  5:217335/134‑1 (MQ=255)                                                                          aCAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGaacaa                                                          <  3:25730/134‑1 (MQ=255)                                                                                 cTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGATTAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGt                                                 <  7:98349/136‑1 (MQ=255)                                                                                   tGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTGGGGCATATCTTc                                                                                                                            <  1:71306/59‑1 (MQ=255)                                                                                   tGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTGGGGCATATCTTc                                                                                                                            <  1:65248/59‑1 (MQ=255)                                                                                   tGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTGGGGCATATCTTc                                                                                                                            >  2:65248/1‑59 (MQ=255)                                                                                   tGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTGGGGCATATCTTc                                                                                                                            >  2:71306/1‑59 (MQ=255)                                                                                      cTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGaa                                             <  2:269889/135‑1 (MQ=255)                                                                                          ctgTCATCTTGTTGCCGTAGTTAATGATTAAGCCACGTTCGGGCATATCTTGGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTc                                        <  7:343691/134‑1 (MQ=255)                                                                                          ccGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTc                                        <  7:239938/136‑1 (MQ=255)                                                                                          ccGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTc                                        <  7:83121/136‑1 (MQ=255)                                                                                             tCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCg                                       >  6:157257/1‑134 (MQ=255)                                                                                             tCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTGGTTTTACAGTTGGGTGTTGCTCCCATTTTGGGCAAGGGGGTACAAAGGGGCGGTGGTAGTTTGGGc                                     >  8:343390/1‑136 (MQ=255)                                                                                                    gttgCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAg                                 <  8:396847/133‑1 (MQ=255)                                                                                                    gttgCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAg                                 <  8:327562/133‑1 (MQ=255)                                                                                                       gCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCg                                                                                                         >  6:197568/1‑58 (MQ=255)                                                                                                       gCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCg                                                                                                         <  5:197568/58‑1 (MQ=255)                                                                                                        ccGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAAc                            <  6:174916/134‑1 (MQ=255)                                                                                                          gttgTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTTTTAGGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTTAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGt                         <  6:324839/132‑1 (MQ=255)                                                                                                            aGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGTGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTg                       <  3:325456/135‑1 (MQ=255)                                                                                                            aGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTg                       <  4:174126/135‑1 (MQ=255)                                                                                                            aGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTg                       <  5:157257/135‑1 (MQ=255)                                                                                                              ttAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGGTTTGCAGTTGGGGGTAGCTCCCGTGTAGGCCAAGGGTGTACAACGGGGCGGTGGAAGTTTCGGCATAGGCAACGGTTGGc                     >  1:391224/1‑135 (MQ=255)                                                                                                                      tAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTaaa             <  3:254642/135‑1 (MQ=255)                                                                                                                      tAAGCCACCTGCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTCGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTaaa             <  6:338092/135‑1 (MQ=255)                                                                                                                         gCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTa                                                                                     <  4:80854/60‑1 (MQ=255)                                                                                                                         gCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTa                                                                                     >  3:80854/1‑60 (MQ=255)                                                                                                                           cACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAAcagcag       <  8:52238/136‑1 (MQ=255)                                                                                                                           cACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAAcagca        <  4:250518/135‑1 (MQ=255)                                                                                                                             ccTTCGGGCATATCTTCGGTTGCATTGTTCCGGCCGTTGATTTCCAGTGGGGTGTAGCTCCCTTGTAGGCCAAGGGGGAACAACGGGGCGGGGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAACagcagc      <  2:202843/135‑1 (MQ=255)                                                                                                                                 cGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTTGGTGTAGCTCCCATGTATGCCAAGGGTGAACAACGGGGCCGGGGAATTTTAGGCATATCCAACGGTTGGCCCATTAAACAGCAGCAACg  >  2:350346/1‑135 (MQ=255)                                                                                                                                 cGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAACAGCAGCAAc   <  7:303653/134‑1 (MQ=255)                                                                                                                                 cGGGCATATCTTCGGTTTCATTGTTACGGCCGTGGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAACAGCAGCAACg  >  3:171327/1‑135 (MQ=255)                                                                                                                                 |                                                                                                                                       CAGGTCGGCCTGGCCGTCCTTGAGCTTCTGGTCATGCTGTTTCGAGTACATGCCGCTGGCTTCGGCCTGGTAAACAAGGCCTTGCCTGGCCGTCATCTTGTTGCCGTAGTTAATGAATAAGCCACCTTCGGGCATATCTTCGGTTTCATTGTTACGGCCGTTGATTTCCAGTTGGGTGTAGCTCCCTTGTAGGCCAAGGGTGAACAACGGGGCGGTGGAAGTTTCGGCATAGCCAACGGTTGGCACAGTAAACAGCAGCAACG  >  ACB122/2037142‑2037404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑