Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,118,179	A→T	5.7%	S164C (AGT→TGT)	CJ019_02010 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,118,179	0	A	T	5.7%	225.8 / 2.7	70	S164C (AGT→TGT)	CJ019_02010	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/28);  new base T (2/2);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

TGGGGCCGCACGTTTTCGCGTGGAGCACCGCTCAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTCCGGTGGTGATGAT  >  ACB122/2118049‑2118310                                                                                                                                   |                                                                                                                                    tGGGGCCGCACGTTTTCGCGTGGAGCACCGCTCAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTa                                                                                                                                 <  6:169601/135‑1 (MQ=255)        gCACGTTTTCGCGTGGAGCACCGCTCAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGccc                                                                                                                          >  6:69247/1‑135 (MQ=255)        gCACGTTTTCGCGTGGAGCACCGCTCAGGCTTGGTCGAGGAGCTGGAAGTGATGGGCTCGGGAGAGGTCGACGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTAACCTGccc                                                                                                                          >  2:172195/1‑135 (MQ=255)           cGTTTTCGCGTGGAGCACCGCTGAGGGGTGGTCGAGGTGCTGGAAGTGATGGGCGCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCg                                                                                                                      <  7:89892/136‑1 (MQ=255)            gTTTTCGCGTGGAGCACCGCTCAGGGGTGGGCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCg                                                                                                                      <  1:29611/135‑1 (MQ=255)                     tGGAGCACCGCTCAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTc                                                                                                             <  4:203687/135‑1 (MQ=255)                                 gaGGGTTGGTCGCGGGGCTGGAAGTGAGGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGa                                                                                                 <  2:199581/134‑1 (MQ=255)                                 cAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGa                                                                                                 <  5:101055/135‑1 (MQ=255)                                 cAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGa                                                                                                 <  6:272341/135‑1 (MQ=255)                                 cAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGa                                                                                                 <  2:350605/135‑1 (MQ=255)                                   gggTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGa                                                                                                 <  2:148143/133‑1 (MQ=255)                                      ttGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAg                                                                                            <  5:358280/135‑1 (MQ=255)                                      ttGGGCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTGGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAg                                                                                            <  3:75623/135‑1 (MQ=255)                                            gAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCa                                                                                       >  5:137539/1‑134 (MQ=255)                                            gAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCa                                                                                       >  3:368743/1‑134 (MQ=255)                                            gAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCa                                                                                       >  6:122354/1‑134 (MQ=255)                                            gAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAg                                                                                      >  7:10567/1‑135 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGTGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGAACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGAGTTTGAGCTAGGTCCAGGATg                                                                                  >  6:262492/1‑134 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCTTTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATg                                                                                  >  6:128073/1‑134 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATg                                                                                  >  6:380849/1‑134 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATg                                                                                  >  1:99274/1‑134 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGa                                                                                 >  8:124829/1‑135 (MQ=255)                                                 gCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGa                                                                                 >  8:99957/1‑135 (MQ=255)                                                   tGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGaaa                                                                               <  4:368743/135‑1 (MQ=255)                                                       aGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGAc                                                                          >  8:86450/1‑136 (MQ=255)                                                          gATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGt                                                                        >  1:381374/1‑135 (MQ=255)                                                             ggggTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGtgct                                                                     <  1:361175/135‑1 (MQ=255)                                                              ggCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGtgctg                                                                    >  2:242400/1‑135 (MQ=255)                                                                   gggAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGa                                                                           <  4:169477/123‑1 (MQ=255)                                                                   gggAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGa                                                                           >  3:169477/1‑123 (MQ=255)                                                                      agagGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCt                                                            >  4:142252/1‑135 (MQ=255)                                                                      agagGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCt                                                            >  2:275411/1‑135 (MQ=255)                                                                           gCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       <  1:224001/135‑1 (MQ=255)                                                                           gCGAGGGCGCCTTGCCCGTGGAGCTGCCTTCACCGCTGGGGCACAGGTTGCACCTGAGGTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGGCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       <  6:240711/135‑1 (MQ=255)                                                                            agAGGGCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGAACAGGTAGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGc                                                     <  7:274523/135‑1 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       >  1:168235/1‑133 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       >  6:241009/1‑133 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       >  4:283810/1‑133 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAAc                                                       >  5:304511/1‑133 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACg                                                      >  7:254705/1‑134 (MQ=255)                                                                             gAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCa                                                    >  8:77348/1‑136 (MQ=255)                                                                                  cGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGa                                                >  8:49257/1‑135 (MQ=255)                                                                                     cTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACaa                                             <  5:11150/135‑1 (MQ=255)                                                                                          ccGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGcc                                        <  5:380849/135‑1 (MQ=255)                                                                                               gAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTc                                   <  7:99957/135‑1 (MQ=255)                                                                                                 gCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGaa                                <  7:86450/136‑1 (MQ=255)                                                                                                  cTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGGCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGaa                                <  5:8890/135‑1 (MQ=255)                                                                                                  cTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGaa                                <  2:381374/135‑1 (MQ=255)                                                                                                  cTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAAc                               >  7:313248/1‑136 (MQ=255)                                                                                                     cATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTg                             >  2:216507/1‑135 (MQ=255)                                                                                                        tCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAATTGACGTGCTGCTGAGGCTTCAACGCAGCGACAATAGCCGTCTCGAACTGCttt                         >  8:184724/1‑136 (MQ=255)                                                                                                           ccGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGc                       <  1:90794/135‑1 (MQ=255)                                                                                                           ccGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGc                       <  2:99274/135‑1 (MQ=255)                                                                                                             gCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTa                     >  3:329783/1‑135 (MQ=255)                                                                                                             gCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTAc                    >  8:39498/1‑136 (MQ=255)                                                                                                               tGGGGCACAGGTTGCACCTGTGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCg                                  >  3:265322/1‑120 (MQ=255)                                                                                                               tGGGGCACAGGTTGCACCTGTGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCg                                  >  3:270381/1‑120 (MQ=255)                                                                                                               tGGGGCACAGGTTGCACCTGTGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCg                                  <  4:265322/120‑1 (MQ=255)                                                                                                               tGGGGCACAGGTTGCACCTGTGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCg                                  <  4:270381/120‑1 (MQ=255)                                                                                                               tGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTAcc                   >  5:259877/1‑135 (MQ=255)                                                                                                                  ggCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGACCTGCTTTGCTACCCtt                >  6:11067/1‑135 (MQ=255)                                                                                                                  ggCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGACCTGCTTTGCTACCCtt                >  4:81768/1‑135 (MQ=255)                                                                                                                  ggCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACACTTc               >  7:397546/1‑136 (MQ=255)                                                                                                                   gCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGTGTCTCATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTc               <  1:172195/135‑1 (MQ=255)                                                                                                                    cacaGGTTGCACCTGAGTTACCTGCCCTTCTTCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTcc              >  3:158174/1‑135 (MQ=255)                                                                                                                    cacaGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTcc              <  1:70344/135‑1 (MQ=255)                                                                                                                    cacaGGTTGCACCTGAGGTACCTGCCCTTCGGCCAGGGTCACATGCGTATGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTcc              <  6:375864/135‑1 (MQ=255)                                                                                                                       aGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTCCggt           >  6:15518/1‑135 (MQ=255)                                                                                                                         gTTGCACTTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTCCggtgg         >  4:35670/1‑135 (MQ=255)                                                                                                                                cTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTCCGGTGGTgatgat  >  6:395656/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                    TGGGGCCGCACGTTTTCGCGTGGAGCACCGCTCAGGGTTGGTCGAGGTGCTGGAAGTGATGGGCTCGGGAGAGGGCGAGGTCGCCTTGCCCGTGGAGCTGCATTCACCGCTGGGGCACAGGTTGCACCTGAGTTACCTGCCCTTCGGCCAGGGTCACATGCGTTTGAGCGAGGTCCAGGATGAAAGTGACGTGCTGCTGAGGCTGCAACGCAGCGACAATAGCCGTGTCGAACTGCTTTGCTACCCTTCCGGTGGTGATGAT  >  ACB122/2118049‑2118310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑