| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,636,079 | A→G | 6.1% | T429A (ACC→GCC) | mltF_2 → | Membrane‑bound lytic murein transglycosylase F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,636,079 | 0 | A | G | 6.1% | 191.8 / 2.7 | 66 | T429A (ACC→GCC) | mltF_2 | Membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base A (24/37); new base G (2/2); total (27/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
CCAAGATCAACGAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTAGCGAAGCGTTAAG > ACB122/2635951‑2636202 | ccAAGATCAACGAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCACCGGCAACCAGTGGTTTTTCCAGACCGAAc > 6:304523/1‑135 (MQ=255)ccAAGATCAACGAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAAc < 8:97493/135‑1 (MQ=255) cAAGATCAACGAGCGTGAGCGCATGGCCTGCGTGCGGGCAGCGTACAAGCTGGGCGCGGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACg < 5:103984/135‑1 (MQ=255) aTCAACGAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATc < 2:291134/135‑1 (MQ=255) aTCAACGAGCGGGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATc < 2:296314/135‑1 (MQ=255) gAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATg > 5:325441/1‑135 (MQ=255) gAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATg < 8:23909/135‑1 (MQ=255) gAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATg > 7:23909/1‑135 (MQ=255) gAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTACAGACCGAACGCATCGCCATgg > 8:340182/1‑136 (MQ=255) tGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCa < 3:111265/135‑1 (MQ=255) tGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCa < 5:141532/135‑1 (MQ=255) tGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCa < 4:304770/135‑1 (MQ=255) gAGCGCATGGCCTTCGTGCTGGCAGTCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAg > 7:25537/1‑136 (MQ=255) aGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGCCGGCGGGGGCTCAACGGCACCCAGTGGTTATTCCAGACCGAACGCATCGCCATGGAGCAAg > 5:314459/1‑135 (MQ=255) gcATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTGTTTACAGCCCGCAAGCATCGCCCAGGAGCAAGTcgg > 8:373163/1‑136 (MQ=255) aTGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGa < 8:243672/136‑1 (MQ=255) ggCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCCACGGCAACCAGTGGTTTTTCCAGACAGAACCCATCGCCATGGTGCAAGTGGGGAt > 3:309835/1‑135 (MQ=255) tGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCgg > 3:278629/1‑135 (MQ=255) gCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGCTGGGACCGGt > 7:252609/1‑135 (MQ=255) aGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCCAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAAcat > 4:19668/1‑133 (MQ=255) ccTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGt < 7:64326/136‑1 (MQ=255) cTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGGTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGt < 1:268954/135‑1 (MQ=255) aCAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTGTTTCCAGACCGCACGCATCGCCCTGCAGCAAGTGGGGATGGGACCGGTCAACgtcgtc > 6:22324/1‑134 (MQ=255) cAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCa < 8:164827/134‑1 (MQ=255) aCCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACa < 3:76610/135‑1 (MQ=255) ggCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATc < 7:223601/135‑1 (MQ=255) ccccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATg > 5:361035/1‑106 (MQ=255) ccccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATg < 6:361035/106‑1 (MQ=255) ccccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGCCCGGTCAACTTGGTGAACAGCATcca > 5:279520/1‑133 (MQ=255) cccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATcaa > 6:129192/1‑134 (MQ=255) ccGAGCTGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCa < 6:41570/94‑1 (MQ=255) ccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGGCGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCgg < 5:272264/111‑1 (MQ=255) ccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGGCGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCAACGCCATGGAGCAAGTGGGGATGGGACCgg > 6:272264/1‑111 (MQ=255) ccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCa > 5:41570/1‑94 (MQ=255) ccGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATcaaca < 2:230176/135‑1 (MQ=255) gggTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGtact < 5:187971/135‑1 (MQ=255) gggTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGtact > 2:232111/1‑135 (MQ=255) ggCCATGCGTGCAGAGGCTCGACGGCGGTGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGc < 3:39508/135‑1 (MQ=255) gTGCAGAGGCTCGACGGCGGGGGCTCACCGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAAcc > 6:46072/1‑135 (MQ=255) gTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATc < 1:46381/110‑1 (MQ=255) gTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATc > 2:46381/1‑110 (MQ=255) gTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAAcc > 4:104292/1‑135 (MQ=255) tGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGGCCGCACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACcg > 2:185726/1‑135 (MQ=255) tGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGGCCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACcg > 2:180126/1‑135 (MQ=255) tGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACcg < 3:253073/135‑1 (MQ=255) tGCAGAGGCTCGAAGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCAAGACCTAACGCATCTCAATGGATCAAGTGGGGATGGGACCGTTAAACTTAGTAAAAAGCATCAAAAAATACTACCTGGCGTTCAACcgc > 8:373225/1‑136 (MQ=255) agagGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGGCCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCTGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGa < 1:180126/135‑1 (MQ=255) agagGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGGCCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGa < 1:185726/135‑1 (MQ=255) gagGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 7:373225/136‑1 (MQ=255) gagGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 8:362291/136‑1 (MQ=255) gagGCGCGACGGCGGGGGCTCAACGGCAACCAGTGGGTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 7:373163/136‑1 (MQ=255) agGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 3:2723/135‑1 (MQ=255) agGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 1:85885/135‑1 (MQ=255) agGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAAc < 4:69020/135‑1 (MQ=255) gCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCTCCATGGAGCAAGTGGGGATGGGACCGGTCATCTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGt < 4:392660/135‑1 (MQ=255) cGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCg > 2:100251/1‑135 (MQ=255) aCGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGc < 3:295527/135‑1 (MQ=255) ggcggGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGGCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACt < 8:25537/136‑1 (MQ=255) gcggGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACt < 1:100251/135‑1 (MQ=255) gcggGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACt < 1:232111/135‑1 (MQ=255) gCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCAACAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCCGCACTGGAGCg > 2:181716/1‑135 (MQ=255) cAACGGCAACCAGTGGTTTTTCCAGACCGCACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCgt > 6:235811/1‑133 (MQ=255) cAACGGCAACCAGTGGTTTTTCCAGACCGAAAGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTa < 8:252609/136‑1 (MQ=255) cGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTAGc < 2:145423/135‑1 (MQ=255) aaCCAGTTGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGGGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTAGCGAAGc < 8:321449/136‑1 (MQ=255) ggTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTAGCGAAGCGTTAAg < 1:179641/135‑1 (MQ=255) | CCAAGATCAACGAGCGTGAGCGCATGGCCTTCGTGCTGGCAGCCTACAACCTGGGCCCCGAGCGGGTTCAGGCCATGCGTGCAGAGGCTCGACGGCGGGGGCTCAACGGCAACCAGTGGTTTTTCCAGACCGAACGCATCGCCATGGAGCAAGTGGGGATGGGACCGGTCAACTTCGTCAACAGCATCAACAAGTACTACCTGGCGTTCAACCGCGAACGTGCGGCACTGGAGCGTGTAGCGAAGCGTTAAG > ACB122/2635951‑2636202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |