Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,652,493	T→C	5.1%	E300E (GAA→GAG)	CJ019_02511 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,652,493	0	T	C	5.1%	259.1 / 2.8	80	E300E (GAA→GAG)	CJ019_02511	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (44/31);  new base C (2/2);  total (47/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

CGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCACCGGT  >  ACB122/2652361‑2652627                                                                                                                                     |                                                                                                                                       cGGGTTGAATGTGACCAGGGCCTGGATGCCGATCATGTTGCGCAGCAGAATGATCAACATCACGCCGATAGGGATCATTACCATGATCATGAAGGTTTGCTGTGTTTGCAGTGGCATGCCGTACATCTAGTATTc                                                                                                                                      <  2:159142/135‑1 (MQ=255)      tGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGACCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGGATTCGAg                                                                                                                                   >  2:349449/1‑133 (MQ=255)         aTGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTACTCGAGGAAGt                                                                                                                              >  3:14904/1‑135 (MQ=255)         aTGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTACTCGAGGAAGt                                                                                                                              >  3:10060/1‑135 (MQ=255)           gtgCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCgg                                                                                                                           >  7:237271/1‑136 (MQ=255)           gtgCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCgg                                                                                                                           >  7:113197/1‑136 (MQ=255)           gtgCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCg                                                                                                                            >  4:193822/1‑135 (MQ=255)           gtgCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCg                                                                                                                            >  2:309073/1‑135 (MQ=255)           gtgCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCg                                                                                                                            >  5:158785/1‑135 (MQ=255)             gCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGc                                                                                                                          <  3:193822/135‑1 (MQ=255)             gCCCAGGGCCTGGATGCCGATCAGGGTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCgg                                                                                                                           <  3:57075/134‑1 (MQ=255)              cccAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTACTCGAGGAAGTCGGCg                                                                                                                         <  4:10060/135‑1 (MQ=255)               ccAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTACTCGAGGAAGTCGGCg                                                                                                                         <  4:14904/134‑1 (MQ=255)                 aGGGCCTGGATGCCGATCAGGTTGCGCAGCCCCAGGATCGCCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCg                                                                                                                      >  1:148720/1‑135 (MQ=255)                 aGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGtcgtcgtcg                                                                                                                      >  3:221710/1‑135 (MQ=255)                  gggCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCgg                                                                                                                     <  5:142805/135‑1 (MQ=255)                    gCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGgtgt                                                                                                                  <  7:103428/136‑1 (MQ=255)                      cTGGAAGCCCATCAGGTTTCGCACCACCAGGATAACCACCACCCCGATAGGGATCAAGACCAATAACATGAAGCTTTGCTTGGTTTTCACTGGCAGGCCCTAAAGCGAGTATTCGAGGAAGTCGGCGTCGgtgt                                                                                                                  >  4:77834/1‑134 (MQ=255)                           tGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCtcgt                                                                                                            >  5:238719/1‑135 (MQ=255)                           tGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGGTCgcgt                                                                                                            >  5:127826/1‑135 (MQ=255)                            gCCGATCAGGTTGCGGAGCACGAGGATCACCAGCACGCTGTTCGGGATCATGAGGATGATCATGAAGGTTTGCTGGGTTTGCAGTGGGAGGCCGTTCAGCGAGTATTTGAGGATGTCGGATTGGGTGTTCtcgtc                                                                                                           <  2:87624/135‑1 (MQ=255)                            gCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCtcgtc                                                                                                           <  5:240375/135‑1 (MQ=255)                                aTCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCgg                                                                                                         >  2:240997/1‑133 (MQ=255)                                aTCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCgg                                                                                                         >  6:365031/1‑133 (MQ=255)                                aTCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGt                                                                                                        >  8:327947/1‑134 (MQ=255)                                aTCAGGTTGCGCAGCACAAGGATAAACATCAAGCCGATCCGCATCATGACAAAGATCAAGAAGAAATTATCTGTTAGAACAGTCACGCCGTACAGCGAGTAATCGAGGAAGTCGCCTTCGGAGTTCTACTCGGta                                                                                                       >  4:56488/1‑134 (MQ=255)                                 tCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGGAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCa                                                                                                      >  4:101510/1‑135 (MQ=255)                                 tCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCa                                                                                                      >  6:171553/1‑135 (MQ=255)                                 tCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTGGTCGTCGGTCa                                                                                                      >  5:361291/1‑135 (MQ=255)                                   aGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCt                                                                                                   >  7:219017/1‑136 (MQ=255)                                     gTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCtt                                                                                                  <  1:383641/135‑1 (MQ=255)                                         cgcAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGcc                                                                                              <  7:327947/135‑1 (MQ=255)                                          gcagcaCCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCa                                                                                             >  5:124428/1‑135 (MQ=255)                                            agcaCCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAgg                                                                                           >  3:368776/1‑135 (MQ=255)                                             gcaCCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGc                                                                                          <  6:238719/135‑1 (MQ=255)                                                 cAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTATCGTCGGTCCGCTTGGCCAGGCGGAt                                                                                      >  4:339166/1‑135 (MQ=255)                                                 cAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTTGTGGTGGGGCGGGTTGGCCAGGCGGAt                                                                                      >  1:364910/1‑135 (MQ=255)                                                        aCCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGGCGGTCAGCTTGGCCAGGCGGATGGCGTTCa                                                                              <  7:153235/136‑1 (MQ=255)                                                         ccAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCa                                                                              <  3:125906/135‑1 (MQ=255)                                                         ccAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCa                                                                              <  6:349030/135‑1 (MQ=255)                                                           aGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGc                                                                                          >  6:25974/1‑121 (MQ=255)                                                           aGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGc                                                                                          <  5:25974/121‑1 (MQ=255)                                                           aGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCAtc                                                                            >  8:4787/1‑135 (MQ=255)                                                           aGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGGCGGTCAGCTTGGCCAGGCGGATGGCGTTCAtc                                                                            >  5:5469/1‑135 (MQ=255)                                                            gCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCtt                                                                                                  >  7:333483/1‑112 (MQ=255)                                                            gCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCtt                                                                                                  <  8:333483/112‑1 (MQ=255)                                                             cACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCAtctc                                                                          <  5:225487/135‑1 (MQ=255)                                                             cACGCCGATCGGGATCATGACCATGATCATGAAGGTGTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCAtctc                                                                          <  4:338475/135‑1 (MQ=255)                                                                gCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGt                                                                                 <  1:127588/125‑1 (MQ=255)                                                                gCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGt                                                                                 >  2:127588/1‑125 (MQ=255)                                                                  cGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCtgt                                                                     >  3:328592/1‑135 (MQ=255)                                                                   gttCGGGATCATGACCATGATCATGAAGGTTTGCGGTGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGAGGGCGTTCATCTCTCtgtt                                                                    <  2:148720/133‑1 (MQ=255)                                                                         gATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGGAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCAt                                                              <  3:101510/135‑1 (MQ=255)                                                                          aTCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATg                                                             <  5:171553/135‑1 (MQ=255)                                                                                       tCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACgg                                                >  3:313820/1‑135 (MQ=255)                                                                                                 ttGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTGCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCcttctt                                      <  4:221710/135‑1 (MQ=255)                                                                                                     tGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTGATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTgccgc                                 >  7:151107/1‑136 (MQ=255)                                                                                                     tGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTgccg                                  >  3:250788/1‑135 (MQ=255)                                                                                                     tGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTgccg                                  >  2:51054/1‑135 (MQ=255)                                                                                                     tGGGTCTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCTTGCTGAAGGTGACGGTGGCCTTCTTgccg                                  >  1:271156/1‑135 (MQ=255)                                                                                                            gCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATgg                                                                                    <  5:11989/78‑1 (MQ=255)                                                                                                            gCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTTCTCGTCGGTCAGCTTGGCCAGGCGGATgg                                                                                    >  6:11989/1‑78 (MQ=255)                                                                                                              aGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGGGACGGTGGCCTTCTTGCCGCCATCGACgg                        <  8:273258/136‑1 (MQ=255)                                                                                                                tGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGt                       <  1:240997/135‑1 (MQ=255)                                                                                                                tGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTTCTCGTCGGTCCGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTGCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGt                       <  4:381990/135‑1 (MQ=255)                                                                                                                   cAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATc                   <  7:169922/136‑1 (MQ=255)                                                                                                                    aGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGa                     >  5:176547/1‑133 (MQ=255)                                                                                                                    aGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATc                   <  5:281898/135‑1 (MQ=255)                                                                                                                     ggCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGGGTTATCGGGGGTGAGCTTGGCCAGGCGGCTGGCGTTCATATCGCGGTTGTTGGTGCTGAAGGTGCCGGTGGCCTTCTTGGCGCCATCGCCGGTGATCa                  >  2:177234/1‑135 (MQ=255)                                                                                                                         gTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCCTCGACGGTGATCAGGTTg             >  7:110279/1‑136 (MQ=255)                                                                                                                             aGCGAGTATTCGAGGAAGTCGGCGTCGGGGGTCTCGGCGGGCAGCTTGGCCCGGCGGGTGGGGGTCCTGGCGGGGGTGTTTCTGCTGAAGGTGCCGGTGGGCGTCGTGGCGCCCTCGACGGGGGGCAGGTTgtcg          >  1:203155/1‑135 (MQ=255)                                                                                                                              gCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTgtcgt         <  3:77834/135‑1 (MQ=255)                                                                                                                               cGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGGCAGCTTGGCCAGGCGGATGGCGGTCATCTCGCTGGTGTTCATGCTGAAGGTGACGGTGGCCGTCGTGCCGCCATCGACGGGGATCAGGTTgtcgtc        >  3:255394/1‑135 (MQ=255)                                                                                                                                gAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCAc      <  8:113197/136‑1 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCACCgg   <  2:65778/135‑1 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCACGTTGTCGTCACCGGt  >  7:70601/1‑136 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTAATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGCTCAGGTTGTCGTCACCgg   >  6:2159/1‑135 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCGGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCACCgg   >  5:334994/1‑135 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGAAGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCACCgg   >  3:193018/1‑135 (MQ=255)                                                                                                                                    aTTCGAGGAAGTCGGCGTCGGGGTTCTCGTCGGTCAGCGTGGCCCGGCGGATGGCGTGCATCTCGCGGTTGTTGATGCTGAAGGTGCCGGTGGCCTTCTTGCCGCCATCGACGGTGCTCAGGTGGTCGTCCCCgg   >  1:385686/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                       CGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCATCGACGGTGATCAGGTTGTCGTCACCGGT  >  ACB122/2652361‑2652627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑