Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	233,250	A→C	22.4%	K157N (AAA→AAC)	hemX →	Protein HemX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	233,250	0	A	C	22.4%	97.3 / 5.7	58	K157N (AAA→AAC)	hemX	Protein HemX
Reads supporting (aligned to +/- strand):  ref base A (29/14);  new base C (2/13);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 5.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCAGCGTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGGCCTGTTCTT  >  ACB122/233115‑233384                                                                                                                                        |                                                                                                                                       ccAGCAGCGTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGGGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCaaa                                                                                                                                        <  8:251857/136‑1 (MQ=255)  cagcagCGTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCaaa                                                                                                                                        <  4:378741/135‑1 (MQ=255)  cagcagCGTCGCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCaaa                                                                                                                                        <  5:27404/135‑1 (MQ=255)   agcagcGTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAg                                                                                                                                       >  5:253852/1‑135 (MQ=255)         gTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGt                                                                                                                                >  7:4658/1‑136 (MQ=255)               gccagcGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAg                                                                                                                           <  5:51079/135‑1 (MQ=255)                ccagcGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAgg                                                                                                                          <  1:232363/135‑1 (MQ=255)                ccagcGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGGGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAgg                                                                                                                          <  2:189102/135‑1 (MQ=255)                    cgcCTGGAAACCGTGCTGGGAGAAAGCCGCAACGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGcc                                                                                                                      <  1:199069/135‑1 (MQ=255)                       cTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCcgcc                                                                                                                   >  3:70724/1‑133 (MQ=255)                       cTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCcgac                                                                                                                   >  5:387937/1‑135 (MQ=255)                           aaaCCGTGCTGGGAGAAAGCCGCAACGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCGGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCcgacga                                                                                                                 <  4:191312/133‑1 (MQ=255)                                   cTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTCCCAAAGCCCTGGTGGAAGGCGCCGACGAGATCCTgcgc                                                                                                       <  3:358859/135‑1 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCTCCTGGCCGAAGCCGAGCCCCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTAGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGACATCCTgcg                                                                                                        >  5:70047/1‑133 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGCGCCCCTCCTGCGGCTGGCTCCCATCCGCCTTTCGGCGTTGCAGGACATCCCCAGTGCCAACGCCCTGGTCGACGGCGCCGACGAGAGCCTGcgcg                                                                                                      >  6:10710/1‑135 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTgcgc                                                                                                       >  8:41196/1‑134 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTgcgc                                                                                                       >  7:119094/1‑134 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTgcg                                                                                                        >  1:81446/1‑133 (MQ=255)                                    tGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGcgcg                                                                                                      >  2:91467/1‑135 (MQ=255)                                            aGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAACGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCg                                                                                             <  8:79796/136‑1 (MQ=255)                                                           cgcCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTCCCACAGCCCTGGTCGAAGGCGGCGACGAGATCCTGCGCGAGCAGAGCGCCCCGGGTGCCTTcg                                                                              >  7:70427/1‑136 (MQ=255)                                                           cgcCTGGCCGAAGCCGAGCACCTCCTCCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCTGACGAGATCCTGCGCGAGCAGCGCGCCCCGGGTGCCTTc                                                                               >  3:266285/1‑135 (MQ=255)                                                                   cGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGCGCAGAGCGACCCGGGTGCCTTCGccgcccg                                                                       >  6:313535/1‑135 (MQ=255)                                                                     aaGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAACGCCCTGGTCGAAGGCGCcgacga                                                                                                                 <  5:246159/91‑1 (MQ=255)                                                                     aaGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCcgacga                                                                                                                 >  6:246159/1‑91 (MQ=255)                                                                        ccGAGCCCCTCCTGCGGCGGGCTACCCTGCGCCTTTCGGCTTTGCAGGACATCACCAGTCCCAAACCCCTGGGCGACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCa                                                                 <  8:386195/136‑1 (MQ=255)                                                                              aCCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGCCCCGGGTGCCTTCGCCGCCCGCGAGCCACTgg                                                            >  5:36331/1‑135 (MQ=255)                                                                               cctcctGCGGCTGGCTACCCTGCGCCTTTCGGGGTGGCTGGACATCACCAGTGCAAACGCCCTGGGCGACGCCGCCGACGAGAACCTGGGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGc                                                           <  5:160827/135‑1 (MQ=255)                                                                               ccgcctGGGGCTGGCTACCCGGCGCCTTTCGGCGTTGCCGGACACCCCCAGGGCCAACGCCCTGGTCGACGGCGCCGACGAGATCCGGCGCGCGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGc                                                           <  1:272710/132‑1 (MQ=255)                                                                               ccgccggCGGCTGGCAACCCGGCGCCTTGCGGCGGGCCAGCACACCCCCAGGCCCAACGCCCTGGGCGACGCCGCCGACGAGATCCTGCGCGAGCAGCGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGc                                                           <  4:331663/129‑1 (MQ=255)                                                                                     gCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGCCGAGATCCTGCGCGAGCAGCGCGACCCGGGTGCCTTCGCCGCGCGCGCGCAACTGGCCCGCAg                                                     >  1:369585/1‑135 (MQ=255)                                                                                     gCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAg                                                     >  5:370370/1‑135 (MQ=255)                                                                                          gggctACCGGGGGCCTTGGGGGGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGTGATCATGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTgg                                                <  1:112382/134‑1 (MQ=255)                                                                                              tACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAACGCGCCGATGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCCCg                                           >  8:301538/1‑136 (MQ=255)                                                                                                    gcgcCTTGCGGCGTTGCAGGACAGCACCAGTGCCAAACCCCTGGTCGAAGGCGCCGACGAGATCCGGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCaa                                      <  1:5684/135‑1 (MQ=255)                                                                                                     cgcCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACa                                    <  8:119094/136‑1 (MQ=255)                                                                                                      gcgtttCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACa                                    <  4:38626/132‑1 (MQ=255)                                                                                                      gcCTTTCGGCGTTGAAGGACATCCCCATTGCAAAACCCGTGTTCGACGCCGCCGACTAGATCGTGCGCTAGATGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACa                                    <  2:26257/135‑1 (MQ=255)                                                                                                      gcCTTTCGGCGGTGCAGGACAACCCCAGGGCCAACGCCCTGGGCGACGGCGCCGACGTGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCaa                                      <  6:362519/133‑1 (MQ=255)                                                                                                        cTTTCGGCGTTGCAGGACATCACCAGTGCCAACGCCCTGGTCGAAGGCGCCGACGAGCTCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCa                                 >  8:60708/1‑136 (MQ=255)                                                                                                        cTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGTCGAGATCCTGCGCGCGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCACCAGc                                  >  2:230274/1‑135 (MQ=255)                                                                                                        cTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGc                                  >  3:86322/1‑135 (MQ=255)                                                                                                              gcggggCAGGACCCCACCAGGGCCAACCCCCTGGCCCACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAg                            <  3:245378/130‑1 (MQ=255)                                                                                                              gCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGCCCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAg                            >  4:85535/1‑135 (MQ=255)                                                                                                                   gccgcacacCACCAGGCCCAACGCCCTGGTCTACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  3:254750/130‑1 (MQ=255)                                                                                                                   gCAGGACATCACCTGTGCCAACGCCGTGGGCGACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  1:22742/135‑1 (MQ=255)                                                                                                                   cccggACATCACCTGGCCCAACGCCCTGGTCTACGGGGCGGACGAGACCCTGCGCTAGCCGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  8:267052/132‑1 (MQ=255)                                                                                                                    cAGGACATCACCAGTGCCAACGCCCTGGTCGACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  2:81446/134‑1 (MQ=255)                                                                                                                    cAGGACATCACCAGTCCCAACGCCTGGGTCGACGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  6:370370/134‑1 (MQ=255)                                                                                                                    cAGGACAGCACCAGTGCAAAAGCCCTGGTCGAAGGCGCCGACTAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  1:69380/134‑1 (MQ=255)                                                                                                                    cAGCACATCCCCAGTGCCAACGCCCTGGGCGACGGCGCCGCCGAGATCCTGCGCGCGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAAcc                       <  1:138590/134‑1 (MQ=255)                                                                                                                     aGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGa                    >  8:101570/1‑136 (MQ=255)                                                                                                                      ggACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGAc                   >  7:190840/1‑136 (MQ=255)                                                                                                                               cAGTGCCACAGCCCTGGTCGACGCGGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTgg           <  1:261626/135‑1 (MQ=255)                                                                                                                               cAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTgg           >  2:217568/1‑135 (MQ=255)                                                                                                                               cAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTgg           >  6:181144/1‑135 (MQ=255)                                                                                                                               cAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTgg           >  3:273227/1‑135 (MQ=255)                                                                                                                                    ccAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCACCAGCACTCAGCAACCGGACCGTACTGGCCTGt      >  5:94014/1‑135 (MQ=255)                                                                                                                                    ccAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGTCCTGt      >  1:159127/1‑135 (MQ=255)                                                                                                                                    ccAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGGCCTGt      >  2:103383/1‑135 (MQ=255)                                                                                                                                    ccAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGGCCTGt      >  4:264137/1‑135 (MQ=255)                                                                                                                                       aaGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGCGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGGCCAGTTCtt  >  8:396143/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                       CCAGCAGCGTCTCAGCCAGCGCCTGGAAACCGTGCTGGGAGAAAGCCGCAAAGAGTGGCGCCTGGCCGAAGCCGAGCACCTCCTGCGGCTGGCTACCCTGCGCCTTTCGGCGTTGCAGGACATCACCAGTGCCAAAGCCCTGGTCGAAGGCGCCGACGAGATCCTGCGCGAGCAGAGCGACCCGGGTGCCTTCGCCGCCCGCGAGCAACTGGCCCGCAGCCTGGCCACGCTCAACAGCACTCAGCAACCGGACCGTACTGGCCTGTTCTT  >  ACB122/233115‑233384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑