Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,758,087 T→G 24.0% intergenic (‑113/+225) CJ019_02600 ← / ← CJ019_02602 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,758,0870TG24.0% 72.7 / 5.5 49intergenic (‑113/+225)CJ019_02600/CJ019_02602hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/29);  new base G (11/2);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 5.86e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGATTG  >  ACB122/2757954‑2758216
                                                                                                                                     |                                                                                                                                 
gggggCGAAACGGAGCAGCATGCTGGGCGAGCTCCTATCGGACAGTTCTGGGGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGtt                                                                                                                                  <  7:368197/132‑1 (MQ=255)
   ggCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCCGCGGGTGGGATGGGGAAAACGGAAGGCCAATCCCGGGCACCCCCCCCGGTgg                                                                                                                                >  4:331740/1‑134 (MQ=255)
           ggAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAg                                                                                                                       <  2:91778/135‑1 (MQ=255)
             agcagcATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCCCCGGGTGGCGGGGGGCACAACGCTTGCCCAGACCCGACACCCCCCCCGGTGGGTAATGTAGGc                                                                                                                     >  2:145072/1‑135 (MQ=255)
              gcagcaTGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCg                                                                                                                   <  8:308353/136‑1 (MQ=255)
               cagcaTGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCgg                                                                                                                  <  8:76793/136‑1 (MQ=255)
                gacaggCTGGGCGATCTCCTATCGGACTGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCgg                                                                                                                  <  1:100077/130‑1 (MQ=255)
                     gCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGGCACACGGATTGCCAAACCCGGACACCCCCCCGGGTGGGGAATTTTGGGCGGCCGGt                                                                                                             >  5:143568/1‑135 (MQ=255)
                                 ccTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCCCCGGTTGGTGGATTTAGGCCGGGACGTCTCCTGACTGGt                                                                                                 >  5:103743/1‑135 (MQ=255)
                                   tATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGAc                                                                                                     <  4:260820/129‑1 (MQ=255)
                                             ttCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGccc                                                                                     >  6:162890/1‑135 (MQ=255)
                                             ttCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCCCCGGGTGGTGAATGTAGGCCGGCCGGTCTCCTGACTGGTGCGGCACGGccc                                                                                     >  3:239023/1‑135 (MQ=255)
                                                tGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCACCGGGTGGCTGGGGGCACACCGATGGCCAATACCGGCCACCCCGCCCGGTGGGGAATGTATGCCGGCCGGTGACCAGACGGGGGCGGCACCGCCCGGc                                                                                  >  3:326366/1‑135 (MQ=255)
                                                  gTGAGGAACGCACGGACAGGCATCGCCGAGCCCCCGCGGGTGGCTGGGGCAACACGGATGGCCCATCCCGGACACCCCGCCGGGTGGTGTATGTAGGCCGGCCGGTGTCCGGACTGGTGGGTCACGGCCCGGcgc                                                                                >  3:353114/1‑133 (MQ=255)
                                                   tGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTcc                                                                               <  4:141948/135‑1 (MQ=255)
                                                         aCGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTc                                                                        <  8:203713/136‑1 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACCCGGCTGGGCCAAACCGGACACCCCCCCGGTTGGTGAATGTAGGCCCGCACGTCTCCTGCATGGTGCGTCACCGCCCGGCGCCGGCCTTcc                                                                       >  8:268670/1‑136 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACCCCCCGCCGGTTGGTGAATGTAGGGCGGCAGGTCTCCTGCCTGGTGCGTGACGGCCCCGCTCCCGCCGTc                                                                        >  1:40281/1‑135 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTcc                                                                       >  7:300195/1‑136 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTc                                                                        >  2:212541/1‑135 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGGGGCTGGGGCAACCCGGATGGCCAATCCCGGCCACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTGTCCTGACGGGTGCGTCCCGGCCCGGCGCCGGCCGTc                                                                        >  2:231503/1‑135 (MQ=255)
                                                          cGCACGGACAGGCATCGCCGAGCCCCAGCGGGGGGCTGGGGCAACACGGATGGCCAATCCCGGCCACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTc                                                                        >  3:163604/1‑135 (MQ=255)
                                                                        tCGCCGAGCCCCCGCGGGTGGCTGGGGCAACACGGATGGCCAATACCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTGTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCCGGCGGCacggc                                                          >  2:7727/1‑131 (MQ=255)
                                                                                 cgcAGCTGGTGGCTGGGGCAACACGGATGGCCAATGCCGGACACGCCGGGGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGAGTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGACCGAGTGGCAt                                                 <  6:147631/133‑1 (MQ=255)
                                                                                     gCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATg                                            <  8:135381/136‑1 (MQ=255)
                                                                                       gggTGGCTGTGGCAACACGGATGGCCAATCCCGGAGACCCCGCCGGTTGGTGAATGGAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATgg                                           <  3:371668/135‑1 (MQ=255)
                                                                                       gggTGGCGGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATgg                                           <  3:372257/135‑1 (MQ=255)
                                                                                          gggCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGGTGGTGAATGTAGGCCGGCAGGGCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCa                                       <  7:299315/135‑1 (MQ=255)
                                                                                             cTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAgg                                     <  6:9437/135‑1 (MQ=255)
                                                                                                 ggCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCgg                                <  7:268670/136‑1 (MQ=255)
                                                                                                   cAACACGGATGGCCAATCCCGGACACCCCGCCGGtt                                                                                                                                  <  5:318570/36‑1 (MQ=255)
                                                                                                   cAACACGGATGGCCAATCCCGGACACCCCGCCGGtt                                                                                                                                  >  6:318570/1‑36 (MQ=255)
                                                                                                       acGGAGGGCCACTCCCGGACTCCCCGCCGGTTGGTGAATGTCGGCCGGCAGGTCTCCTGACTGGGGCGTCACGGCCCGGCTGCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCt                           <  3:331740/135‑1 (MQ=255)
                                                                                                            tGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGt                      <  4:192078/135‑1 (MQ=255)
                                                                                                            tGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGt                      <  5:162890/135‑1 (MQ=255)
                                                                                                              gCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGc                   >  7:137467/1‑136 (MQ=255)
                                                                                                                 aaTCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCgg                 <  1:231503/135‑1 (MQ=255)
                                                                                                                 aaTCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCgg                 <  1:87751/135‑1 (MQ=255)
                                                                                                                         acacCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTc        <  8:300195/136‑1 (MQ=255)
                                                                                                                          cacCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTc        <  5:119996/135‑1 (MQ=255)
                                                                                                                          cacCCCGCCGGGTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTc        <  3:82205/135‑1 (MQ=255)
                                                                                                                           acCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCgg                 >  2:301438/1‑125 (MQ=255)
                                                                                                                           acCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCgg                 <  1:301438/125‑1 (MQ=255)
                                                                                                                              ccgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCTGCACCTACAGTTGCGGGGGCAGTTCCGAt    <  4:239023/135‑1 (MQ=255)
                                                                                                                              ccgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAtt   <  7:52786/136‑1 (MQ=255)
                                                                                                                              ccgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCGTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAt    <  6:294213/135‑1 (MQ=255)
                                                                                                                               cgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCTGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAtt   <  6:364951/135‑1 (MQ=255)
                                                                                                                               cgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAtt   <  6:143568/135‑1 (MQ=255)
                                                                                                                               cgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAtt   <  6:103743/135‑1 (MQ=255)
                                                                                                                               cgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGAtt   <  3:159011/135‑1 (MQ=255)
                                                                                                                               cgccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGGTCCGAtt   <  5:166855/135‑1 (MQ=255)
                                                                                                                                gccgGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGATTg  >  5:193529/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GGTGGCGAAACGGAGCAGCATGCTGGGCGATCTCCTATCGGACAGTTCTGGTGAGGAACGCACGGACAGGCATCGCCGAGCCCCAGCGGGTGGCTGGGGCAACACGGATGGCCAATCCCGGACACCCCGCCGGTTGGTGAATGTAGGCCGGCAGGTCTCCTGACTGGTGCGTCACGGCCCGGCTCCGGCCTTCCCGGGCGGCAGGCCCAGTGGCATCGATGGAGCAGGCTCGGCACCTACAGTTGCGGGGGCAGTTCCGATTG  >  ACB122/2757954‑2758216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑