Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,805,671 G→C 6.4% E89Q (GAA→CAA)  rplT → 50S ribosomal protein L20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,805,6710GC 6.4% 182.2 / 2.4 59E89Q (GAA→CAA) rplT50S ribosomal protein L20
Reads supporting (aligned to +/- strand):  ref base G (43/11);  new base C (2/2);  total (46/13)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTACCGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCAT  >  ACB122/2805542‑2805806
                                                                                                                                 |                                                                                                                                       
gCCTACCGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAAt                                                                                                                                     <  2:6920/134‑1 (MQ=255)
     ccGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACTCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGAcc                                                                                                                                >  1:293607/1‑134 (MQ=255)
     ccGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGAcc                                                                                                                                >  5:131732/1‑134 (MQ=255)
     ccGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGAcc                                                                                                                                >  4:211481/1‑134 (MQ=255)
     ccGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCg                                                                                                                               >  7:46677/1‑135 (MQ=255)
     ccGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCg                                                                                                                               >  7:272707/1‑135 (MQ=255)
      cgcgACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGAGTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCg                                                                                                                               >  6:333781/1‑134 (MQ=255)
       gcgACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGCTCGAAATCGACCg                                                                                                                               >  3:34048/1‑133 (MQ=255)
       gcgACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGAGGCTCGCTCGTATCAACGCCGGAGCCCGCACCAACGGTCTTTCCTACCGCCGTCGGGTTGCTGGCCTGCAAAAGGCGTCGCTCTACTTCGACCg                                                                                                                               >  2:290525/1‑133 (MQ=255)
        cgACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAg                                                                                                                           >  8:63225/1‑136 (MQ=255)
             gTCGCCAGAAGAAGCGTCACATCCGCGCACTGAGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCAGATTGCAAGCCTGAAAAAGGCTACGATCGAACTCGCACGTAACGtt                                                                                                                        >  7:268784/1‑134 (MQ=255)
                   agaagaAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCCCCAACGGTCTGTCCTACAGCCGGCGGATTTCTGGCCAGAAAAAGGCATCGAAGCTAATACACCCTAAGGTTCAGGCt                                                                                                                  >  4:208732/1‑134 (MQ=255)
                                         aCTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTTGCTGATCTGGCAGTGAACGAAAAAgc                                                                                           >  3:198237/1‑135 (MQ=255)
                                         aCTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAgc                                                                                           >  1:217085/1‑135 (MQ=255)
                                         aCTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAgc                                                                                           >  3:173496/1‑135 (MQ=255)
                                         aCTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCCAAATCGACCGTAAGGTTCTGGCTGATCTg                                                                                                            <  7:356072/118‑1 (MQ=255)
                                         aCTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCCAAATCGACCGTAAGGTTCTGGCTGATCTg                                                                                                            >  8:356072/1‑118 (MQ=255)
                                              ggATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGAc                                                                                                                                 <  7:32405/92‑1 (MQ=255)
                                              ggATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGAc                                                                                                                                 >  8:32405/1‑92 (MQ=255)
                                              ggATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGt                                                                                      >  1:77923/1‑135 (MQ=255)
                                                aTCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGttt                                                                                    >  4:22638/1‑135 (MQ=255)
                                                     tCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGGGAACGAAAAAGCGGCGGTtgctgc                                                                               >  5:50572/1‑135 (MQ=255)
                                                      cGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCg                                                                              >  2:328064/1‑135 (MQ=255)
                                                        tATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTGTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGAt                                                                            >  4:198279/1‑135 (MQ=255)
                                                          tCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTg                                                                          >  5:164627/1‑135 (MQ=255)
                                                            aaCGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTc                                                                        <  8:46677/135‑1 (MQ=255)
                                                                      cccGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCt                                                               >  3:328088/1‑134 (MQ=255)
                                                                      cccGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCt                                                               >  1:344910/1‑134 (MQ=255)
                                                                      cccGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCCGCGTTTTCTGCGCTTGTGGAGAAAGCt                                                               >  5:40171/1‑134 (MQ=255)
                                                                             cAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGt                                                       <  2:217085/135‑1 (MQ=255)
                                                                                          tACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGCTTGTCGAGAAATCTAAAGCCATTCTGGCTTAAGTa                                          >  4:12351/1‑135 (MQ=255)
                                                                                          tACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTa                                          >  3:203239/1‑135 (MQ=255)
                                                                                                    tGATTGCTTGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACa                                 >  4:159620/1‑134 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCg                                                                       >  1:6687/1‑96 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCg                                                                       >  6:394051/1‑96 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCg                                                                       <  2:6687/96‑1 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCg                                                                       <  5:394051/96‑1 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTa                                                              <  5:103567/105‑1 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTa                                                              >  6:103567/1‑105 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  1:217087/1‑135 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  5:140822/1‑135 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  2:175608/1‑135 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  3:198825/1‑135 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  3:380541/1‑135 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACa                                 >  1:25752/1‑134 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACa                                 >  1:291518/1‑134 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAAt                               >  7:202222/1‑136 (MQ=255)
                                                                                                    tGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTAGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGCTTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAAt                               >  7:234315/1‑136 (MQ=255)
                                                                                                     gATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAAt                               >  8:90251/1‑135 (MQ=255)
                                                                                                     gATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCCGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACaa                                >  5:157080/1‑134 (MQ=255)
                                                                                                       ttGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCa                             >  1:350871/1‑135 (MQ=255)
                                                                                                          cTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCAcc                           <  4:173496/134‑1 (MQ=255)
                                                                                                          cTGGCCGGAAAACGGCTTCGCTCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGGGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCAcc                           <  4:182798/134‑1 (MQ=255)
                                                                                                           tGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGcc                                                         >  7:210126/1‑103 (MQ=255)
                                                                                                           tGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGcc                                                         <  8:210126/103‑1 (MQ=255)
                                                                                                               cTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCATTCTGGCTTAAGTACCCGCGACAATCACCcggcgg                     >  2:88441/1‑135 (MQ=255)
                                                                                                                   aaaaGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGc                  <  6:131732/134‑1 (MQ=255)
                                                                                                                     aaGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAg               >  3:291524/1‑135 (MQ=255)
                                                                                                                         cTTCGATCCAACTCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCt           <  5:333781/135‑1 (MQ=255)
                                                                                                                               tcgaAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCACTgaccg      >  4:204262/1‑130 (MQ=255)
                                                                                                                                 gaAATCGACCGTAAGGTTCTAGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCAt  <  8:234315/136‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GCCTACCGCGACCGTCGCCAGAAGAAGCGTCAGTTCCGCGCACTGTGGATCGCTCGTATCAACGCCGGTGCCCGCACCAACGGTCTGTCCTACAGCCGTCTGATTGCTGGCCTGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCAT  >  ACB122/2805542‑2805806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑