Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,880,012	G→T	6.5%	T13T (ACC→ACA)	CJ019_02740 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,880,012	0	G	T	6.5%	200.0 / 4.4	62	T13T (ACC→ACA)	CJ019_02740	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/31);  new base T (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

GCATCAAGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGGTGTTATTTC  >  ACB122/2879885‑2880146                                                                                                                                |                                                                                                                                       gCATCAAGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAgtggt                                                                                                                                >  7:255783/1‑136 (MQ=255)  cATCAAGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAgtggt                                                                                                                                <  1:1681/135‑1 (MQ=255)    tCAAGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGa                                                                                                                              <  3:7717/135‑1 (MQ=255)       aGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGAtc                                                                                                                            >  1:37674/1‑134 (MQ=255)       aGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTGGTCTTGCAGGGTAGTGGTTAtc                                                                                                                            >  5:110543/1‑134 (MQ=255)       aGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGATCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGAtc                                                                                                                            >  1:76352/1‑134 (MQ=255)        ggTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  8:34007/136‑1 (MQ=255)        ggTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  8:164467/136‑1 (MQ=255)         gTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  3:361167/135‑1 (MQ=255)            gAGCCTTTCACGTCACATGCTAGCAATTCTGTAAGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtcatca                                                                                                                      <  6:223449/135‑1 (MQ=255)               ccTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         >  3:11352/1‑129 (MQ=255)               ccTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  4:11352/129‑1 (MQ=255)               ccTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGc                                                                                                                  >  7:240442/1‑136 (MQ=255)               ccTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGc                                                                                                                  <  7:267428/136‑1 (MQ=255)                cTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         >  1:368637/1‑128 (MQ=255)                cTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  2:368637/128‑1 (MQ=255)                cTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGc                                                                                                                  <  2:204904/135‑1 (MQ=255)                cTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGc                                                                                                                  >  8:150576/1‑135 (MQ=255)                 tttCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGGGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCt                                                                                                                 <  2:9626/135‑1 (MQ=255)                    cACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCta                                                                                                                <  4:383422/133‑1 (MQ=255)                            aTGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATaa                                                                                                     >  7:138800/1‑136 (MQ=255)                             tGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCACAATCGAAGAAGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGATATAAAGAtata                                                                                                      >  1:282989/1‑134 (MQ=255)                                   cAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACAttt                                                                                                <  5:99908/134‑1 (MQ=255)                                      ttCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAgcg                                                                                           >  8:65537/1‑136 (MQ=255)                                      ttCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTATTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAgc                                                                                            >  3:42384/1‑135 (MQ=255)                                             cgttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGTGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCa                                                                                      >  6:350339/1‑134 (MQ=255)                                             cgttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGTGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCAt                                                                                     >  6:344271/1‑135 (MQ=255)                                             cgttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         >  8:245245/1‑99 (MQ=255)                                             cgttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCtca                                                                                                                         <  7:245245/99‑1 (MQ=255)                                             cgttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCAt                                                                                     >  2:201111/1‑135 (MQ=255)                                              gttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTAATCATTGCTATATAGATATAACATTTCAGCGCTCCAt                                                                                     >  1:376528/1‑134 (MQ=255)                                               ttcgttGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTcc                                                                                  <  7:150576/136‑1 (MQ=255)                                                    tGCCTGATTGTTTATGTACAAAGAATGGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTg                                                                              >  1:29478/1‑135 (MQ=255)                                                     gCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGt                                                                             <  8:10228/135‑1 (MQ=255)                                                      cccGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGt                                                                             <  1:201111/134‑1 (MQ=255)                                                          aTTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCt                                                                        <  4:79879/135‑1 (MQ=255)                                                          aTTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTAGAAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATGATTGCTTTATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCt                                                                        <  2:282989/135‑1 (MQ=255)                                                                     aCAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGACGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAatgat                                                              <  5:266593/134‑1 (MQ=255)                                                                       aaaGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAg                                                          <  7:123177/136‑1 (MQ=255)                                                                        aaGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAg                                                          <  4:240900/135‑1 (MQ=255)                                                                        aaGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAg                                                          <  1:279058/135‑1 (MQ=255)                                                                                 gCGTGAACGTTAGCTCGCAGAATCGAAGATTCACCTTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATCACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATc                                                 <  6:139225/135‑1 (MQ=255)                                                                                    tGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTTTCTGa                                              >  1:248743/1‑135 (MQ=255)                                                                                    tGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGa                                              >  6:66357/1‑135 (MQ=255)                                                                                      aaCGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            <  8:255783/135‑1 (MQ=255)                                                                                       aCGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGTGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            <  5:350339/134‑1 (MQ=255)                                                                                       aCGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGTGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            <  5:344271/134‑1 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAatga                                                               >  8:348639/1‑114 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAatga                                                               <  7:348639/114‑1 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            >  3:167815/1‑133 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            >  1:276263/1‑133 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTc                                           >  7:179700/1‑134 (MQ=255)                                                                                        cGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTGTGTTTTCTGGTTAATGATGGAGTCATTTATCTGAtt                                            >  3:391468/1‑133 (MQ=255)                                                                                          ttAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGc                                       <  8:240442/136‑1 (MQ=255)                                                                                             gCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGt                                     >  2:58244/1‑135 (MQ=255)                                                                                                      aTCGAAGATGCACAGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTa                            >  5:207648/1‑135 (MQ=255)                                                                                                                 aCCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTaaaaaa                 <  2:37674/135‑1 (MQ=255)                                                                                                                  ccGTTGTCGTGCAGGGTAGTGGTGATCTCATCATTTCTATATAGATATAACTTTTCAGCGCTCCATTCCTTTGTTTTCTTGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAg                <  2:376528/135‑1 (MQ=255)                                                                                                                        tCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGgt          >  6:149511/1‑135 (MQ=255)                                                                                                                             cAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGgtgt        <  1:68269/132‑1 (MQ=255)                                                                                                                              aGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCg                                      <  4:200638/101‑1 (MQ=255)                                                                                                                              aGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCg                                      >  3:200638/1‑101 (MQ=255)                                                                                                                                ggTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGGTGTTATTTc  >  6:48761/1‑135 (MQ=255)                                                                                                                                ggTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGGTGTTATTTc  >  6:200553/1‑135 (MQ=255)                                                                                                                                |                                                                                                                                       GCATCAAGGTCGAGCCTTTCACGTCACATGCTAGCAATTCTGTACGTTCGTTGCCCGATTGTTTATGTACAAAGAATAGAGCGTGAACGTTAGCTCGCAGAATCGAAGATGCACCGTTGTCTTGCAGGGTAGTGGTGATCTCATCATTGCTATATAGATATAACATTTCAGCGCTCCATTCCTTTGTTTTCTGGTTAATGATGGAGTCATTTATCTGATTCGAGCGTCATGCGCTACTGGTAAAAAAGCCAGGTGTTATTTC  >  ACB122/2879885‑2880146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑