Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,176,299 G→A 9.0% R301H (CGT→CAT)  carC_2 → Caffeyl‑CoA reductase‑Etf complex subunit CarC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,176,2990GA 9.0% 133.1 / 2.9 44R301H (CGT→CAT) carC_2Caffeyl‑CoA reductase‑Etf complex subunit CarC
Reads supporting (aligned to +/- strand):  ref base G (23/17);  new base A (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCGCGCGCCGCGCCCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGAC  >  ACB122/3176167‑3176433
                                                                                                                                    |                                                                                                                                      
gTGCCGCGCGCCGCGCCCACGACATCGCCTGCGACTATGCTCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTc                                                                                                                                      <  3:272576/135‑1 (MQ=255)
gTGCCGCGCGCCGCGCCCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTc                                                                                                                                      <  3:103308/135‑1 (MQ=255)
   ccgcgcgccgcgcCCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTGATGCTGGCCGCCAACATGATGGACCTGCACGTGGTGCGTCTgg                                                                                                                                   >  6:104547/1‑135 (MQ=255)
    cgcgcgccgcgcCCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCCTGGTGGCCGACAACATGATGGACCAGCACGTGGTGCGGCTGGCg                                                                                                                                 >  7:272424/1‑136 (MQ=255)
          ccgcgccCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTg                                                                                                                            <  3:122180/135‑1 (MQ=255)
                 cACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTgcg                                                                                                                    <  7:290589/136‑1 (MQ=255)
                        tCGCCTGCGACTATGCGCGCTCGCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGGTTCATGCTGGCCGACATCATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTggg                                                                                                              <  1:2254/135‑1 (MQ=255)
                          gCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGCCAACATGCTGGCCCTGCCCGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTg                                                                                                            >  5:386617/1‑135 (MQ=255)
                                  cTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGTGGTCTGGCACTGCGCCTGGGTGCTCGCCCa                                                                                                    >  4:46331/1‑135 (MQ=255)
                                      gcgcgcACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGc                                                                                                <  6:316467/135‑1 (MQ=255)
                                             cccGGGACGCCTTTGGCAAGCCGCGGGGCGAGCACCAGGGCGGGGGTGTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGgcgcg                                                                                         <  5:85864/135‑1 (MQ=255)
                                                           ggCACGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTc                                                                            <  6:103800/134‑1 (MQ=255)
                                                           ggCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGCCCAGGGCCGGCGCGCCAATGGCGATTc                                                                            >  7:55837/1‑134 (MQ=255)
                                                                    cTGGGCGAGCACCAGGGCGTGGGTTTCCTGCTGGCCGCCAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGc                                                                   >  5:159907/1‑134 (MQ=255)
                                                                    cTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCCCTGCGCCTGGGTGCTCGACCCGGGCCGGCGCGCCAATGTCGATTCGAGCATGGc                                                                   >  3:66175/1‑134 (MQ=255)
                                                                              aCCCGGGCGTTGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCATCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCa                                                        <  3:4769/135‑1 (MQ=255)
                                                                              aCCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCATCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCa                                                        <  3:237/135‑1 (MQ=255)
                                                                               ccAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGAGTCGAGCATGGCCAAGGTGATCAg                                                       >  3:246328/1‑135 (MQ=255)
                                                                                 tgggCGTGGGTTTCATGCGGGCCGACAACATGATGGACCTGCACGGGGTGCGGCGGGCGGTCTGGCACTGCGCCGGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAgcg                                                     <  5:123067/134‑1 (MQ=255)
                                                                                 agtgCGTGGGTTGCATGATGCACCACAACATGAGGCACCTGCGCGTGGTGGGTCGGGGGGGCTGGCACGGCGCGTGGTTGCTGTACCTGGGCCGGCGCGCCAATGTCGATTCGACCATGGCCACGGTGATCAgcg                                                     <  2:150577/132‑1 (MQ=255)
                                                                                          gTTTCATGCTGGCCGACAACATGATGTACCTTAACGTGGTGCATCTGGCGGTCTTGCACTTCGCCTGGGTGCTCTACCAGTGCCGGCGACCCAATGTCGATTAGAGGATTGACAACGTGATCAGCGCCGAGgcga                                            >  4:4769/1‑134 (MQ=255)
                                                                                          gTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCAGGGCCAAGGTGATCAGCGCCGCGgcgcg                                           >  7:130426/1‑135 (MQ=255)
                                                                                          gTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCATCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCACGGCCGGCGCCCCAATGTCGATTCGATCATGGCCAAGGTGATCAGCGCCGCGgagc                                            >  4:237/1‑135 (MQ=255)
                                                                                                       cGACAACATGCTGGACCTGCCCGGGGGGCGGCGGGCGTGCGGGCACTGGGCCGGGGGGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggtggt                               <  6:43756/135‑1 (MQ=255)
                                                                                                       cGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTg                                                                                                                            <  1:105099/42‑1 (MQ=255)
                                                                                                       cGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTg                                                                                                                            >  2:105099/1‑42 (MQ=255)
                                                                                                        gACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTc                                                                            >  2:339168/1‑89 (MQ=255)
                                                                                                        gACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTc                                                                            <  1:339168/89‑1 (MQ=255)
                                                                                                        gACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTc                              >  8:307597/1‑135 (MQ=255)
                                                                                                                 atgGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGc                     <  8:290413/135‑1 (MQ=255)
                                                                                                                      ccggCACGTGGGGCGGCGGGCGGGCGGGCACTGCGGCTGGGGGCGCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAg               <  8:130426/133‑1 (MQ=255)
                                                                                                                          cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGTGCGCCAATGTCGATTCGAGCATGGCCAAGGTGAt                                                          <  4:259741/89‑1 (MQ=255)
                                                                                                                          cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGTGCGCCAATGTCGATTCGAGCATGGCCAAGGTGAt                                                          >  3:259741/1‑89 (MQ=255)
                                                                                                                          cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGCGGCGCTGTGGCGGGTGGTCGCTCGTTGCGTCCAGGTa            >  1:318633/1‑135 (MQ=255)
                                                                                                                          cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTAt           >  7:11705/1‑136 (MQ=255)
                                                                                                                          cACGTGCTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCACCGCCGAGGCGCACTGGAGGGTGGTCGATCGTTGCGTCCACGTa            >  1:207069/1‑135 (MQ=255)
                                                                                                                             gtggtgCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTg         >  5:388097/1‑135 (MQ=255)
                                                                                                                               ggtgCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTggg       >  1:313166/1‑135 (MQ=255)
                                                                                                                                  gCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCCAGGTGATCAGCGCCGAGGCGCTGTGGCGGGGGGTCGATCGTTGCGTCCAGGTATTGGGTGGa   >  8:342315/1‑136 (MQ=255)
                                                                                                                                  gCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGCGGCGCTGTGGCGGGTGGTCGATCGTTGCGGGCAGGGATTGGGTgg    >  5:39252/1‑135 (MQ=255)
                                                                                                                                  gCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTgg    >  6:358869/1‑135 (MQ=255)
                                                                                                                                  gCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTgg    <  5:358869/135‑1 (MQ=255)
                                                                                                                                  gCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGa   >  7:227673/1‑136 (MQ=255)
                                                                                                                                   cGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGa   >  6:145952/1‑135 (MQ=255)
                                                                                                                                    gTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGGGGTCGATCGTTGCGTGCACGGATTGGGTGGAc  >  5:329765/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GTGCCGCGCGCCGCGCCCACGACATCGCCTGCGACTATGCGCGCACCCGGGACGCCTTTGGCAAGCCGCTGGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGAC  >  ACB122/3176167‑3176433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑