| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 381,772 | T→G | 23.5% | L198R (CTG→CGG) | soxB → | Sarcosine oxidase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 381,772 | 0 | T | G | 23.5% | 86.3 / 2.9 | 60 | L198R (CTG→CGG) | soxB | Sarcosine oxidase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (17/22); new base G (12/3); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGC > ACB122/381642‑381906 | gCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCAGGGc > 3:97252/1‑135 (MQ=255) cGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGGCCGTCACGATGCCGTGGCCTGGGGGGTGGCCCCCGGAGCCGCCCCCCCGGGCgg > 5:356063/1‑134 (MQ=255) cGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGCTGCCGTGGCCTGGGGCTTGGCCCGCGCTGCCCCCGCCCGGGGCgg > 1:129661/1‑134 (MQ=255) cGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACCCCCGGGGCGt > 2:146525/1‑135 (MQ=255) tCCCGTACCTGGCCTGCTCGATGAACACCCGCTCCCCGATCATCGGCGCCCCCGTACCGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTGCGCCCGCGCTGCCGCCGCCCGGGGCGTCGAccg > 7:70609/1‑135 (MQ=255) tCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCCCCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGCTGCCGTGGCCTGGGGCGTCGCCCGCGCTGCCGCCGCCCGGGGCGGCGGcc > 6:114910/1‑135 (MQ=255) tCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGTCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGccc > 4:311894/1‑135 (MQ=255) gTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGAt < 2:308844/135‑1 (MQ=255) gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGt > 8:110951/1‑135 (MQ=255) gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCAGCCGACGCCCTGGGCGTCCACC‑TGATCCAGCAAACCGAAg > 1:6411/1‑134 (MQ=255) gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCCCTGCCGCCGCCCTGGGCGTAGCCC‑GGATGCCGCAAACCgcatgg > 8:77033/1‑131 (MQ=255) gaagaaCACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTAGCCCCCGCTGCCCACCCCCAGGGGCGCGACC‑GGGACCAGCCAAccccaa > 4:338472/1‑130 (MQ=255) cGCTACCCGATCCTCGGCGCCACCGTCCAGCGCCGTGGTGGGGTTGCCCGTCACGATGCCGGGGCCTGGGGCTGCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCtgc < 6:296444/135‑3 (MQ=255) cGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCCCGCTGCCGACGCCCTGGGCGTCGACC‑AGATCCAGCAAACCGAAGTGATCGGcagac > 7:322760/1‑132 (MQ=255) cGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTAGCCCGCGCTGCCCCCCCCCAGGGCGGGGCCC‑CGATACCGCAAAACCAAAAGATCGGcata > 3:55364/1‑132 (MQ=255) cGCTACCCGATCCTCGGCGCAACCGGACCGCGCGGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTc < 3:49521/135‑1 (MQ=255) aCCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTAGCCCGCGCTGCCGACCCCCAGGGCGGGGGCC‑TGGAACAGCAAAACGCAATGATGGGCGTACGc > 2:247274/1‑134 (MQ=255) gcgcCACCGTCCAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACgg < 5:85907/133‑1 (MQ=255) gcgcCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCGGGGCGGCGCCC‑GGCTCCCGCAAACCGAAGTGCTCGGCTTCCGCCAGGCAAACGgcgc > 8:66919/1‑136 (MQ=255) cgcCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGGGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGgcgc < 3:176702/135‑1 (MQ=255) gcCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCGGGGCGGCGGCC‑GGATGCAGCCAACCGAAGTGATCCGCTTCCGCCAGGAAAACGGCGCgg > 8:19792/1‑136 (MQ=255) ccACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCGGGGCGGCGCCC‑GGCTCCCGCAAACCCCAGTGATCGGCGTCCGCCAGGACACCGGCCCggg > 7:303465/1‑135 (MQ=255) cACCGTACAGCGCCGTGGGGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCTACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGt < 1:247274/135‑1 (MQ=255) aCCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTc < 4:142351/135‑1 (MQ=255) aCCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGAGGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCa < 7:332458/136‑1 (MQ=255) cGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGGTTCGCCCGCGCTGCCGCCCCCCGGGGCGGCGGCC‑GGGTCCCGCCAACCCAAGTGATCCGGGTGCGCCACGAAAACGGCGCggggat > 1:143740/1‑131 (MQ=255) cgcCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCTGGGGGTCGCCC‑TGGTCCAGCCAACCGAAGTGATCGGCTTGCGCCAGGGAAAACGCGCGGGCATGCGtgt‑g > 1:47011/1‑135 (MQ=255) gcCGTGGTGGCGTTGCCCGGCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑gg < 4:246110/135‑1 (MQ=255) cGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCCGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGaaa > 7:309554/1‑136 (MQ=255) ggtggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCGGGGCGGCGGCC‑GGGTGCCGCCAACCGAAGTGGTCGGCGTGCGCCAGGAAAACGGCGCGGGCATCGGTGT‑GGGAAcc > 8:269855/1‑135 (MQ=255) gtggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACca < 3:144495/135‑1 (MQ=255) gtggCGTTGCCCGGCACGATGCCGTGGCCTGGGGCTTCGCCCGGGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAACCCGAAGTGAGCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACca < 3:138293/135‑1 (MQ=255) ggggggTTGCCCGTCACGTTGCGGGGGCCTGGGGCTGCGCCCGCGGTGCCTACGCCCTGGGCGTCGACC‑TCACCCCCAAAACCTAAGTTATCGGCTTCCGAAAGTAAAACGGCGCGGTCATCGGTGT‑GGAAACca < 2:390335/130‑1 (MQ=255) tggCGTTGCCCGTCACGATGCCGTGGCCTGGGGGTTGGCCCGCGCTTCCGACCCCCGGGGCGTGGCCCGTGGACCACCAAACC‑AATTGATAAGCTTCCGGCACGAAAACGGCGCGGTGACTGGGGTGGGAAACca > 6:335628/1‑135 (MQ=255) tggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGGCGCCCGGGGCGGGGCCC‑CGCTCCACCCAACCGCACTGTTCGGCGTACGCCAGGGAAAACGCGCGGGGATCGGGGG‑GGGAACca > 4:391371/1‑134 (MQ=255) tggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCGGGGCGTGGACC‑GGGTCCCCCCAACCGAAGTGATCGGCGTCCGCCAGGAAAACGGCGCGGTCCTCGGTGT‑GGGAACca > 5:109486/1‑134 (MQ=255) tggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGGCC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGGAAACGGCGCGGTCATCGGTGT‑GGaaaacca > 8:249980/1‑131 (MQ=255) gggCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCGGCGCTGCCGACCCCCTAGGCTTCTACC‑TGATCCAGCAAACCGAAGTGAGGGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACca < 3:282297/133‑1 (MQ=255) ttGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGCCC‑TGCTCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGGAACCAACAAAg > 6:194994/1‑135 (MQ=255) tCACGATGCCGTGGCCTGGGGCTTCGCCAGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATc > 8:381665/1‑136 (MQ=255) cGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGCCGCCCTGGGGGTCGCCC‑TGATCCAGGAAACCGAAGTGCTAGGCGTCCGCAAGGAAAACGGCGCGGTCCTCGGTGT‑GGAAACCCACAAAGGCTTCATCgg > 1:17822/1‑135 (MQ=255) cGATGCCGTGGCCTGGGGCTTAGCCCGCGCTGCCGACGCCCTGGGCGTCGCCC‑TGCTCCCGGAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCCACAAAGGCGTCAACGgc > 8:376293/1‑136 (MQ=255) cGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCAGGGCGTCGACC‑GGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAAAGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATAGGCGCCAAg > 7:51231/1‑136 (MQ=255) ccTGGGGCTTCGCCCGCGCTGCCGACCCCCTGGGCGTCGACC‑AGATCCAGCAAACCGAAGTGATCGGCATACGCAAGGAAAACGGCGCGGTCATACGTGT‑GGAAACCAACAAAGGCGTCAACGGCGCCAcgcgcg > 3:118827/1‑135 (MQ=255) ggCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGCCC‑TGATGCAGCCAACCGCAGTGATCGGCGTACGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGc > 2:84520/1‑135 (MQ=255) ggCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑GGATCCAGCAAACCCAAGTGATCGGCGTCCGCAAGGAAAACGGCGCGGTCCTCGGGGT‑GGAAACCAACAAAGGCTTCATCGGCGCCACGCGCCTCGGc > 1:248776/1‑135 (MQ=255) cGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTc < 7:269855/136‑1 (MQ=255) gCCCGCGGCCCCGCCGCCCGGCGCGGCGACC‑TGAGCCACCAACCCCCAGAGATCGGCCGCCCCCCACAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTc < 1:175169/135‑1 (MQ=255) gCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTgg < 1:237514/133‑1 (MQ=255) gCCCGCGCGGCCGACGCCCGGGGCGTCGACC‑TGATCCACCAAACCGAAGTGATCGGCTTCCGCACGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTc < 6:71792/135‑1 (MQ=255) gCCCGCGCGGCCGACGCCCGGGGCGTCGACC‑TGACCCAGCAAACCGAAGTGAGCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTc < 3:338472/135‑1 (MQ=255) ccGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACaaa < 5:308674/100‑1 (MQ=255) ccGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACaaa > 6:308674/1‑100 (MQ=255) tGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCgg > 7:254728/1‑135 (MQ=255) gACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGAGCGGCTTCCGCAAGTAAAACGGCGCGGTCATGGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAAc < 1:146525/135‑1 (MQ=255) aCGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACt < 4:97252/135‑1 (MQ=255) ccTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCgg < 4:55364/135‑1 (MQ=255) ccTGGGCGACGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCgg < 5:104040/135‑1 (MQ=255) gtGGGCGTCTACT‑TTATCCACCAACCCTCAGTTATCGGCTTCCGAAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAATGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGGCACCGCCGGTAACTCCggg < 6:356063/134‑1 (MQ=255) cTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCggg < 3:168619/135‑1 (MQ=255) cTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCggg < 2:46369/135‑1 (MQ=255) tGGGCGTCGACC‑TGATCCTGCAAACCGAAGTGAGCGGCTTCCGCAAGTAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGc < 1:115398/135‑1 (MQ=255) | GCCGAAATCCCGTACCTGGACTGCTCGAAGAACACCCGCTACCCGATCCTCGGCGCCACCGTACAGCGCCGTGGTGGCGTTGCCCGTCACGATGCCGTGGCCTGGGGCTTCGCCCGCGCTGCCGACGCCCTGGGCGTCGACC‑TGATCCAGCAAACCGAAGTGATCGGCTTCCGCAAGGAAAACGGCGCGGTCATCGGTGT‑GGAAACCAACAAAGGCTTCATCGGCGCCAAGCGCGTCGGCGTGGTCACCGCCGGTAACTCCGGGC > ACB122/381642‑381906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |