Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,228,754 C→T 7.5% R264R (CGC→CGT)  garD_1 → Galactarate dehydratase (L‑threo‑forming)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,228,7540CT 7.5% 216.9 / 6.1 67R264R (CGC→CGT) garD_1Galactarate dehydratase (L‑threo‑forming)
Reads supporting (aligned to +/- strand):  ref base C (36/26);  new base T (2/3);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

CCAGGGCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCGGTGGTACCGCCAT  >  ACB122/3228625‑3228885
                                                                                                                                 |                                                                                                                                   
cccgggCCGGCAGGCGAGGGCGCAACTGCGCGCCTTAACCATCCAAGGGATCGGGGGCACTTCGAAACCCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGTCAGcc                                                                                                                                <  5:44751/132‑1 (MQ=255)
   gggCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCgg                                                                                                                              >  4:11375/1‑134 (MQ=255)
   gggCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCgg                                                                                                                              >  2:83682/1‑134 (MQ=255)
     gCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAATACCATCGCTGCCGGTATCGCCCAGGTCAAGGCTCTGTTGGCCGAGGCCGATCAGGTACGGCGCCAGCCGGTCa                                                                                                                           >  5:225543/1‑135 (MQ=255)
     gCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCa                                                                                                                           >  4:104624/1‑135 (MQ=255)
      ccTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGTCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGt                                                                                                                         <  8:230330/136‑1 (MQ=255)
       cTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGt                                                                                                                         <  4:25467/135‑1 (MQ=255)
          cAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGcc                                                                                                                      <  1:350109/135‑1 (MQ=255)
             gCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCg                                                                                                                    >  3:133235/1‑134 (MQ=255)
                   gcgcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtg                                                                                                             >  3:373345/1‑135 (MQ=255)
                    cgcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtgg                                                                                                            <  1:63942/135‑1 (MQ=255)
                    cgcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtgg                                                                                                            <  2:98596/135‑1 (MQ=255)
                    cgcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGGCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtgg                                                                                                            <  4:133235/135‑1 (MQ=255)
                     gcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGcc                                                                                                                                    <  1:21912/110‑1 (MQ=255)
                     gcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGcc                                                                                                                                    >  2:21912/1‑110 (MQ=255)
                     gcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtggt                                                                                                           >  3:390529/1‑135 (MQ=255)
                     gcAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtggt                                                                                                           >  4:45818/1‑135 (MQ=255)
                       aaCTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtggtgg                                                                                                         >  5:132590/1‑135 (MQ=255)
                               gcCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCt                                                                                                  >  6:245364/1‑134 (MQ=255)
                                    tACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTg                                                                                            >  3:197452/1‑135 (MQ=255)
                                      ccATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTgcgg                                                                                         >  7:335048/1‑136 (MQ=255)
                                            aaGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGTTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCt                                                                                    >  5:25223/1‑135 (MQ=255)
                                            aaGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCt                                                                                    <  6:149391/135‑1 (MQ=255)
                                              gggATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCCGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCa                                                                                  >  2:255374/1‑135 (MQ=255)
                                              gggATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtgg                                                                                                            <  2:54598/109‑1 (MQ=255)
                                              gggATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCtgg                                                                                                            >  1:54598/1‑109 (MQ=255)
                                               ggATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGa                                                                                <  8:191872/136‑1 (MQ=255)
                                                    gggggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGc                                                                            >  8:202027/1‑135 (MQ=255)
                                                      gggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGTCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTAt                                                                         >  7:122760/1‑136 (MQ=255)
                                                      gggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGTCAGCCGGTCAGTGCACGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTa                                                                          >  3:12990/1‑135 (MQ=255)
                                                      gggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTgc                                                                                           >  4:126189/1‑118 (MQ=255)
                                                      gggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTgc                                                                                           <  3:126189/118‑1 (MQ=255)
                                                      gggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTa                                                                          >  4:281693/1‑135 (MQ=255)
                                                       ggCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTAt                                                                         >  1:85729/1‑135 (MQ=255)
                                                            ttCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCggg                                                                    <  3:286424/135‑1 (MQ=255)
                                                                 aaaCCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              <  8:335048/136‑1 (MQ=255)
                                                                  aaCCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              <  4:197452/135‑1 (MQ=255)
                                                                  aaCCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              <  6:212172/135‑1 (MQ=255)
                                                                  aaCCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGAGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              <  3:266869/135‑1 (MQ=255)
                                                                      aTCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCa                                                         <  7:200171/136‑1 (MQ=255)
                                                                      aTCGCTGCCGGTATCGCCCAGGGCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCa                                                         <  8:42907/136‑1 (MQ=255)
                                                                         gCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAAcc                                                      >  8:25002/1‑136 (MQ=255)
                                                                             ccGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCg                                                    <  7:25002/134‑1 (MQ=255)
                                                                              cGGTATCGCCCAGGGCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCg                                                    <  5:315293/133‑1 (MQ=255)
                                                                                   tCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTgg                                             <  8:244360/135‑1 (MQ=255)
                                                                                   tCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTgg                                             <  7:202027/135‑1 (MQ=255)
                                                                                    cGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTgg                                             <  4:373345/134‑1 (MQ=255)
                                                                                              aaGGCGCTGTTGGCCGAGGCCAATCAGGTACTGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGtt                                  >  3:213888/1‑135 (MQ=255)
                                                                                                 gcgcTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCCCCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGAc                               >  2:92603/1‑135 (MQ=255)
                                                                                                  cgcTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGAcc                              >  6:121078/1‑135 (MQ=255)
                                                                                                   gcTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCg                             >  5:150675/1‑135 (MQ=255)
                                                                                                   gcTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGc                            >  8:62378/1‑136 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCCCCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCtgg                        >  2:390174/1‑135 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              >  2:64789/1‑97 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCAc                                                              <  1:64789/97‑1 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCa                                                         >  1:254914/1‑102 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCa                                                         <  2:254914/102‑1 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCCCTGGGCAATGCCGTTGACCGCCtgg                        >  3:207082/1‑135 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCtgg                        >  5:168816/1‑135 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCtgg                        >  1:348993/1‑135 (MQ=255)
                                                                                                        tGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCACCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGCTGGCTATTCGGGGATCACCGACAACCCGGCCCTGGGCAATGCCGTTGACCGCCtgg                        >  5:118641/1‑135 (MQ=255)
                                                                                                           ccGAGGCCAATCAGGTACGGCGTCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGgc                    <  8:122760/136‑1 (MQ=255)
                                                                                                            cGAGGCCAATCAGGTACGGCGTCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGgc                    <  4:12990/135‑1 (MQ=255)
                                                                                                               ggCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGgccgcc                >  7:308463/1‑136 (MQ=255)
                                                                                                                  cAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCggt             >  7:259139/1‑136 (MQ=255)
                                                                                                                       aGGTACGGCGCCAGCCGGTAAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCGGTGGTa         >  3:290270/1‑135 (MQ=255)
                                                                                                                        ggTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCGGTGGTa         <  2:356029/134‑1 (MQ=255)
                                                                                                                            cGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGcggcgg                                                                                      >  2:395126/1‑53 (MQ=255)
                                                                                                                            cGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGcggcgg                                                                                      <  1:395126/53‑1 (MQ=255)
                                                                                                                              gcgcCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCGGTGGTACCGCCAt  >  3:249674/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                   
CCAGGGCCTGCAGGCGAGTGCGCAACTGCGCGCCTTTACCATCCAAGGGATCGGGGGCACTTCGAAAACCATCGCTGCCGGTATCGCCCAGGTCAAGGCGCTGTTGGCCGAGGCCAATCAGGTACGGCGCCAGCCGGTCAGTGCCCGCCACCTGGTGGTCGGCCTGCAGTGCGGCGGCTCAGATGGCTATTCGGGGATCACCGCCAACCCGGCACTGGGCAATGCCGTTGACCGCCTGGTGGCCGCCGGTGGTACCGCCAT  >  ACB122/3228625‑3228885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑