Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,388,835 C→T 5.6% A112V (GCG→GTG)  CJ019_03197 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,388,8350CT 5.6% 227.0 / 3.4 71A112V (GCG→GTG) CJ019_03197hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/47);  new base T (2/2);  total (22/49)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GCGCACCTGGGCATCATGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAGCCCGACCTACACCGTGAC  >  ACB122/3388702‑3388969
                                                                                                                                     |                                                                                                                                      
gcgcACCTGGGCATCATGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGgcgc                                                                                                                                      <  7:194065/136‑1 (MQ=255)
 cgcACCTGGGCAGCATGGGCCAAGACCGTGCCGCGGCGCGGGGCGCCGCCCAACGCTTTGGCGGCAGTGAGAAGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGgcgc                                                                                                                                      <  6:28124/135‑1 (MQ=255)
       ggggCATCATGTGCGACGACCTGGACGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTgccag                                                                                                                                <  1:360071/134‑1 (MQ=255)
        gggCATCATGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTgccag                                                                                                                                >  6:347914/1‑134 (MQ=255)
          gCATCATGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTgccagc                                                                                                                               <  3:211014/133‑1 (MQ=255)
            atcatGTGCTAAGACGTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCaaa                                                                                                                           <  4:257197/135‑1 (MQ=255)
             tcatGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAAt                                                                                                                          <  5:354931/135‑1 (MQ=255)
             tcatGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         >  7:118390/1‑136 (MQ=255)
              catGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATTGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         <  4:369323/135‑1 (MQ=255)
              catGTGCGAAGACATGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         <  2:285884/135‑1 (MQ=255)
              catGGGCGAAGAGTGGGATGCGGGGGTGGCGGCGGCCCAACGCTTTGGCGTCAGTGAGATGCCGCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         <  8:390715/135‑1 (MQ=255)
                   gCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGc                                                                                                                   >  8:978/1‑136 (MQ=255)
                       aGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCa                                                                                                                >  4:194924/1‑135 (MQ=255)
                        gACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAg                                                                                                               <  6:235598/135‑1 (MQ=255)
                          ccTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGc                                                                                                             <  5:185192/135‑1 (MQ=255)
                                      gcTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGCTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGa                                                                                                 >  2:261544/1‑135 (MQ=255)
                                      gcTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCAc                                                                                                           <  3:259426/125‑1 (MQ=255)
                                      gcTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCAc                                                                                                           >  4:259426/1‑125 (MQ=255)
                                      gcTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGa                                                                                                 >  2:108550/1‑135 (MQ=255)
                                           ccgccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgc                                                                                            <  6:22327/135‑1 (MQ=255)
                                           ccgccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgc                                                                                            <  6:39691/135‑1 (MQ=255)
                                            cgccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTgccagc                                                                                                                               <  6:286405/99‑1 (MQ=255)
                                            cgccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTgccagc                                                                                                                               >  5:286405/1‑99 (MQ=255)
                                             gccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgcgcg                                                                                         <  8:113713/136‑1 (MQ=255)
                                             gccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgcgcg                                                                                         <  8:2819/136‑1 (MQ=255)
                                             gccgccCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgcgc                                                                                          <  4:306415/135‑1 (MQ=255)
                                                   cAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAg                                                                                    <  4:160861/135‑1 (MQ=255)
                                                    aaCGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAgg                                                                                   <  3:302513/135‑1 (MQ=255)
                                                              cGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCa                                                                           >  6:164643/1‑133 (MQ=255)
                                                               gTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGa                                                                        <  4:210410/135‑1 (MQ=255)
                                                               gTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGa                                                                        <  2:60359/135‑1 (MQ=255)
                                                               gTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGa                                                                        <  2:20744/135‑1 (MQ=255)
                                                                  aGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCg                                                                     <  8:36942/135‑1 (MQ=255)
                                                                   gTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCg                                                                     >  1:126772/1‑134 (MQ=255)
                                                                     gagaTGCCCCAGGGCCGCACCTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATCCGTAGCACAGGCACTGGCCATCGACCCTCGCGCGGAGGTGCAGGGATGAGCGTGa                                                                  >  1:340956/1‑135 (MQ=255)
                                                                       gaTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  6:253146/135‑1 (MQ=255)
                                                                       gaTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  6:309385/135‑1 (MQ=255)
                                                                       gaTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  5:338284/135‑1 (MQ=255)
                                                                       gaTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  5:347914/135‑1 (MQ=255)
                                                                       gaTGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  4:156510/135‑1 (MQ=255)
                                                                              cAGGGCGGCAACTGGCTGCGCCTGCCTGATGGGCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAATTGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGt                                                         <  2:340956/135‑1 (MQ=255)
                                                                                  gCCGAAACTGGCTGCGCCTGCCTGCTGGCCGGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTg                                                     <  1:377643/135‑1 (MQ=255)
                                                                                   ccGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTgg                                                    >  6:103182/1‑135 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGTGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  7:298022/117‑1 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGTGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                >  8:298022/1‑117 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         <  8:392427/60‑1 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATg                                                                                                                         >  7:392427/1‑60 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGAt                                              >  3:250449/1‑135 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGGTCATCCAGGTGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                >  8:298020/1‑117 (MQ=255)
                                                                                         cTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGATCATCCAGGTGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  7:298020/117‑1 (MQ=255)
                                                                                         cTGGCCGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGAt                                              >  4:195783/1‑135 (MQ=255)
                                                                                             cTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGccc                                          <  2:221674/135‑1 (MQ=255)
                                                                                             cTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGccc                                          <  5:128441/135‑1 (MQ=255)
                                                                                             cTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGc                                            <  5:269919/133‑1 (MQ=255)
                                                                                                   ctgcctgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAg                                    <  3:262735/135‑1 (MQ=255)
                                                                                                   ctgcctgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAg                                    <  4:217493/135‑1 (MQ=255)
                                                                                                   ctgcctgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAg                                    <  5:70261/135‑1 (MQ=255)
                                                                                                       ctgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGa                                <  1:261544/135‑1 (MQ=255)
                                                                                                       ctgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGa                                <  2:63265/135‑1 (MQ=255)
                                                                                                        tgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                >  7:36623/1‑102 (MQ=255)
                                                                                                        tgATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGAtt                                                                <  8:36623/102‑1 (MQ=255)
                                                                                                                tGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATc                      <  7:266204/136‑1 (MQ=255)
                                                                                                                  gCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGTCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATc                      <  8:193248/134‑1 (MQ=255)
                                                                                                                  gCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATc                      <  7:135444/134‑1 (MQ=255)
                                                                                                                   cGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATc                      <  2:371424/133‑1 (MQ=255)
                                                                                                                   cGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATc                      <  2:14604/133‑1 (MQ=255)
                                                                                                                   cGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAg                    <  3:390616/135‑1 (MQ=255)
                                                                                                                   cGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAg                    <  4:257293/135‑1 (MQ=255)
                                                                                                                   cGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACAGGGACGATGCCCGCGAAGGCGATGAGTGCATCAGc                   >  7:334434/1‑136 (MQ=255)
                                                                                                                                ccAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgcgcg                                                                                         >  5:13879/1‑53 (MQ=255)
                                                                                                                                ccAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCcgcgcg                                                                                         <  6:13879/53‑1 (MQ=255)
                                                                                                                                ccAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCTGGCATGAGCGTGATGGAAAAGTCCTGGGACGATGCCCGAGAAGGCGATGAGTGCATCAGCCCGACCTACAcc       >  5:47765/1‑135 (MQ=255)
                                                                                                                                 cAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAGCCCGACCTACACCGt     >  7:232014/1‑136 (MQ=255)
                                                                                                                                    gcgcTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAGCCCGACCTACACCGTGAc  <  8:118390/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GCGCACCTGGGCATCATGTGCGAAGACCTGGAAGCGGCGCTGGCCGCCGCCCAACGCTTTGGCGTCAGTGAGATGCCCCAGGGCCGCAACTGGCTGCGCCTGCCTGATGGCCTGGCGGTGGAGTTCATCCAGGCGCTGCCAGCCAAATGCGTAGCCCAGGCACTGGCCATCGACCCGCGCGCGGAGGTGCAGGCATGAGCGTGATTGAAAAGTACTGGGACGATGCCCGCGAAGGCGATGAGTGCATCAGCCCGACCTACACCGTGAC  >  ACB122/3388702‑3388969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑