Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,475,364 C→A 8.0% R1206L (CGG→CTG)  CJ019_03296 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,475,3640CA 8.0% 171.6 / 4.5 58R1206L (CGG→CTG) CJ019_03296hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/32);  new base A (3/2);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCAGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTGCGGTAGTGCTG  >  ACB122/3475231‑3475494
                                                                                                                                     |                                                                                                                                  
ggTCAGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGGGTTGCTGAATGCCGTCCCGGTCGTCGCCCCGCGCGCGCGCCTTACTGGGCAGCAGGCCCCCCCg                                                                                                                                   >  5:232684/1‑135 (MQ=255)
    aGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCt                                                                                                                               <  4:283827/135‑1 (MQ=255)
    aGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTg                                                                                                                              >  8:218147/1‑136 (MQ=255)
    aGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACAGCCCt                                                                                                                               >  4:148372/1‑135 (MQ=255)
    aGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACAGCCCt                                                                                                                               >  4:142258/1‑135 (MQ=255)
    aGCTGGACCCCGCCGAAGTGCCGCACGACAAGGCTACCGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCt                                                                                                                               <  5:344667/135‑1 (MQ=255)
     gCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTg                                                                                                                              >  2:107408/1‑135 (MQ=255)
     gCTGGACCCCGCCGACGTTGCGCACGACACGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCt                                                                                                                               <  1:346653/134‑1 (MQ=255)
           ccccGCCGATGTGGCGCACGAAAAGGCGTCCGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGGCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGt                                                                                                                        <  4:380805/135‑1 (MQ=255)
             cccctcaaGTGGCGCACGCCACGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCg                                                                                                                      <  5:23017/129‑1 (MQ=255)
              cgccgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGCGCGCTCTCATTCATGCGCAGCAGGCCCCCCCGCCCTGGGAAGTCGa                                                                                                                     >  6:51732/1‑135 (MQ=255)
                ccgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAac                                                                                                                   <  3:113514/135‑1 (MQ=255)
                ccgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAac                                                                                                                   <  4:358113/135‑1 (MQ=255)
                ccgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACAGCCCTGGGAAGTCGAac                                                                                                                   <  3:142258/135‑1 (MQ=255)
                ccgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACAGCCCTGGGAAGTCGAac                                                                                                                   <  3:148372/135‑1 (MQ=255)
                 cgAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGAGGTGTTCAAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGATCTCATTCATCCGCAGCAGGCCACACAGCCCTGGGAAGTCGAaca                                                                                                                  >  6:179835/1‑135 (MQ=255)
                     gTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGt                                                                                                              <  3:299035/135‑1 (MQ=255)
                      tggcgcACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGa                                                                                                            <  8:340868/134‑1 (MQ=255)
                       tGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGa                                                                                                            <  3:356784/135‑1 (MQ=255)
                       tGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGa                                                                                                            >  6:182109/1‑135 (MQ=255)
                           cACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCgt                                                                                                       <  7:381733/136‑1 (MQ=255)
                            aCGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCgtg                                                                                                      >  7:114153/1‑136 (MQ=255)
                                cacggCTAACGCGGCCGCGGGCGGTGGTCCAACTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGcc                                                                                                  <  8:366132/133‑1 (MQ=255)
                                 aaGGCTTACGCGGCCGCTGGCTGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAAAACGGTGACCCGTGTGCCg                                                                                                 >  8:260365/1‑136 (MQ=255)
                                 aaGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGCCCCGAGCGCTGTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGGAGTCGACCACGGTGACCCGTGTGcc                                                                                                  >  6:240494/1‑135 (MQ=255)
                                          gcgGCCGCTGGCTGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTg                                                                                        <  8:278876/136‑1 (MQ=255)
                                           cgGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAggg                                                                                          <  2:272695/133‑1 (MQ=255)
                                           cgGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAggg                                                                                          >  1:272695/1‑133 (MQ=255)
                                           cgGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGGCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAggggg                                                                                        <  5:169050/135‑3 (MQ=255)
                                                 cTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGacgccg                                                                                  >  4:378485/1‑135 (MQ=255)
                                                 cTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGacgacg                                                                                  >  3:358095/1‑135 (MQ=255)
                                                 cTGGCGGTGTTCCAGCTGAAGCCTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCGCGGCTTCATGCGCCGCCGGCCCCACCGCCCAGGGAAGTCGAACACCGGGACCCCTGTGCCGCTCAGGGTgcccccg                                                                                  >  6:226493/1‑129 (MQ=255)
                                                     cGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAg                                                                             <  7:37641/136‑1 (MQ=255)
                                                                 tGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCg                                                                  <  4:46790/135‑1 (MQ=255)
                                                                 tGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCg                                                                  <  4:317172/135‑1 (MQ=255)
                                                                 tGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCg                                                                  <  4:111750/135‑1 (MQ=255)
                                                                 tGAAGCGTTGCTGAATGCCGTCCAGGGCGTCGACCCGAGCGCTCTCATACATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGACCACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCg                                                                  <  5:130336/135‑1 (MQ=255)
                                                                  gAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCgg                                                                 >  2:52402/1‑135 (MQ=255)
                                                                    aGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGgc                                                                <  4:358095/134‑1 (MQ=255)
                                                                       gTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCCCCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGCCGTCCAGGCGCGCGCCCGGCGCgg                                                            >  4:174963/1‑135 (MQ=255)
                                                                           cTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTAATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGCGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTg                                                       >  8:119010/1‑136 (MQ=255)
                                                                                     gccAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGGCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGcc                                             <  8:80046/135‑1 (MQ=255)
                                                                                        aGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCCCCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCATGGTGCCGCCGTCCAGGCGCGCGCCCGGCGCGGTGCAGGTGCCCGGCCAg                                           >  5:124363/1‑135 (MQ=255)
                                                                                        aGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCGGGTGCCCGGCCAg                                           >  2:337737/1‑135 (MQ=255)
                                                                                          gtcgtcGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCg                                        <  8:143272/136‑1 (MQ=255)
                                                                                           tcgtcgACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGGGACCCGTGTGCCGCGCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCg                                        <  5:51732/135‑1 (MQ=255)
                                                                                                                ttcatGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCtgctgc                     <  5:182109/133‑1 (MQ=255)
                                                                                                                  catGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGc                  <  1:183090/134‑1 (MQ=255)
                                                                                                                      cgcAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCGAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTg             <  3:294845/135‑1 (MQ=255)
                                                                                                                       gcagcaGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTg             <  1:107408/134‑1 (MQ=255)
                                                                                                                        cagcagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCgt                                                                                                       <  5:67314/43‑1 (MQ=255)
                                                                                                                        cagcagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCgt                                                                                                       >  6:67314/1‑43 (MQ=255)
                                                                                                                        cagcagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCg                                        >  2:149002/1‑106 (MQ=255)
                                                                                                                        cagcagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCg                                        <  1:149002/106‑1 (MQ=255)
                                                                                                                        cagcagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTGCg           >  4:175081/1‑135 (MQ=255)
                                                                                                                           cagGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTGCGGTa        <  3:24062/135‑1 (MQ=255)
                                                                                                                            agGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCCGCGCGGTGCTGGTGCCCGGCCAGGCGCAGCGGTTCCAGCTGCTGCGGCCGGTGCGGTAGt      >  7:283502/1‑136 (MQ=255)
                                                                                                                                 ccACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTGCGGTAGtgctg  <  6:352389/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
GGTCAGCTTGACCCCGCCGAAGTTGCGCACGACAAGGCTTACGCGGCCGCTGGCGGTGTTCCAGCTGAAGCGTTGCTGAATGCCGTCCAGGTCGTCGACCCGAGCGCTCTCATTCATGCGCAGCAGGCCCCACCGCCCTGGGAAGTCGAACACGGTGACCCGTGTGCCGCTCAGGGTGACGACGTCCAGGCGCGCGCCCGGCGCGGTGCTGGTGCCCGGCCAGGCGAAGCGGTTCCAGCTGCTGCGGCCGTTGCGGTAGTGCTG  >  ACB122/3475231‑3475494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑