Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,483,024	G→A	7.0%	A462V (GCC→GTC)	clpV1 ←	Protein ClpV1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,483,024	0	G	A	7.0%	183.5 / 3.5	57	A462V (GCC→GTC)	clpV1	Protein ClpV1
Reads supporting (aligned to +/- strand):  ref base G (30/23);  new base A (2/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

CGTCGGCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGT  >  ACB122/3482890‑3483159                                                                                                                                       |                                                                                                                                        cGTCGGCATCGTTGGCGGCGGCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCggg                                                                                                                                         <  3:305153/135‑1 (MQ=255)    cGGCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCAt                                                                                                                                      >  2:277799/1‑135 (MQ=255)    cGGCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATg                                                                                                                                     >  8:197620/1‑136 (MQ=255)      gCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCAGTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGt                                                                                                                                   <  7:43069/136‑1 (MQ=255)      gCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGGTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATgg                                                                                                                                    <  2:287830/135‑1 (MQ=255)         tCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGt                                                                                                                                <  7:197620/136‑1 (MQ=255)          cGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGt                                                                                                                                <  3:240227/135‑1 (MQ=255)             tGGCGGCGTCCAGCGCCTTGAGCTGATCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGcc                                                                                                                             <  2:37748/135‑1 (MQ=255)                 ggcggcCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCtt                                                                                                                         <  4:312823/135‑1 (MQ=255)                             cTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTg                                                                                                             >  4:259602/1‑135 (MQ=255)                                  gCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGa                                                                                                        >  1:99539/1‑135 (MQ=255)                                   cTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAg                                                                                                       >  3:372470/1‑135 (MQ=255)                                         gACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGCGCACggc                                                                                                 >  1:167530/1‑133 (MQ=255)                                         gACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTc                                                                                                 >  7:381835/1‑135 (MQ=255)                                         gACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGACATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGt                                                                                                  >  1:249257/1‑134 (MQ=255)                                          aCCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGACATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCg                                                                                                >  5:304496/1‑135 (MQ=255)                                              gTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTc                                                                                           <  8:360052/136‑1 (MQ=255)                                                 ccAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCg                                                                                         >  1:12627/1‑135 (MQ=255)                                                       ccTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCa                                                                                   >  6:76967/1‑135 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTg                                                                              >  2:96999/1‑135 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGt                                                                             >  8:328560/1‑136 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGt                                                                             >  7:397180/1‑136 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGt                                                                             >  7:306955/1‑136 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGAGTTCCGCCGGCAGGGTg                                                                              >  5:353220/1‑135 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTAGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTg                                                                              >  5:260542/1‑135 (MQ=255)                                                            tgcCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGAGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCCCGTCGATTTCCGACTGGAGGTTGt                                                                             >  7:362757/1‑136 (MQ=255)                                                            tgcCACTGGGCATTGAGCAGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGAGCCCCTTGCCGGCTTCGTGGCCGTGCACGTCGATTTCCGCCAGCAGGTTg                                                                              >  5:65918/1‑135 (MQ=255)                                                             gcCACTGGGCATTGAGCTGCTGATCCTGCTGCTGCTCGGCCTGCACGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCATCTTGGCCGCGCACGTCCATTTGCGCCTTCAGGtt                                                                               >  1:352985/1‑133 (MQ=255)                                                               cACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGACATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTc                                                                            <  6:304496/134‑1 (MQ=255)                                                                     gCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCt                                                                       <  6:182635/133‑1 (MQ=255)                                                                        ttGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcgg                                                                  <  1:277799/135‑1 (MQ=255)                                                                        ttGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcgg                                                                  <  6:110982/135‑1 (MQ=255)                                                                        ttGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcgg                                                                  <  3:228111/135‑1 (MQ=255)                                                                        ttGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCAGGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcgg                                                                  <  3:310838/135‑1 (MQ=255)                                                                        ttGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGACATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcgg                                                                  <  2:249257/135‑1 (MQ=255)                                                                           aGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcggcg                                                                <  4:229229/134‑1 (MQ=255)                                                                           aGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCcggcg                                                                >  1:335683/1‑134 (MQ=255)                                                                                   ttCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTTCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTTCAGGTTGTAGATCTGCCGGCGGCAGTCtt                                                        >  4:224804/1‑134 (MQ=255)                                                                                   ttCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAgtcgtc                                                       >  6:306620/1‑135 (MQ=255)                                                                                   ttCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCtt                                                        >  2:29043/1‑134 (MQ=255)                                                                                   ttCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCtt                                                        >  6:383195/1‑134 (MQ=255)                                                                                    tCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGTAGGTTGTCGATCTGCCGGCGGCAGTCTTCCa                                                     >  7:211356/1‑136 (MQ=255)                                                                                    tCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTcc                                                      <  4:149142/135‑1 (MQ=255)                                                                                        gctgctgctCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTg                                                  >  3:275442/1‑135 (MQ=255)                                                                                          tgctgctCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTgcgc                                               <  7:338293/136‑1 (MQ=255)                                                                                                      tGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGaa                                    <  5:258459/135‑1 (MQ=255)                                                                                                      tGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGaa                                    <  5:12427/135‑1 (MQ=255)                                                                                                           ggCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTg                               <  3:327894/135‑1 (MQ=255)                                                                                                                ccTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTTCGCCa                          >  4:325948/1‑135 (MQ=255)                                                                                                                   gCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAggg                       >  8:272818/1‑135 (MQ=255)                                                                                                                   gCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTAGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTTCGCCAgg                        >  5:75965/1‑134 (MQ=255)                                                                                                                     aGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCa                     <  3:91415/135‑1 (MQ=255)                                                                                                                       cGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAAt                   <  4:372470/135‑1 (MQ=255)                                                                                                                             cAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCCGGTTGTCGATCTGCCGGCGGCCGTCTTGCCGTGCGCCCGGCCGCGCAGATTTCGCCAGGGCAATgcgcgc             >  6:242819/1‑135 (MQ=255)                                                                                                                             cAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATgcgcgc             >  5:278204/1‑135 (MQ=255)                                                                                                                               ggcggcGGGCATGGTCGTGCCCCGTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATgcgcgcgc           <  5:5516/135‑1 (MQ=255)                                                                                                                               ggcggcGGGAATGGGCGTGACCCGTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGAGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATgcgcgcgc           <  2:94093/135‑1 (MQ=255)                                                                                                                                 gggagggCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTTCGCCAGGGCAATGCGCGCGCAg         <  2:352985/131‑1 (MQ=255)                                                                                                                                   gcggGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGc       <  2:91580/135‑1 (MQ=255)                                                                                                                                       gCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCgtgt  >  7:186066/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                        CGTCGGCATCGTTGGCGGCGTCCAGCGCCTTGAGCTGTTCGACCAGTTCCAGCTCCTGCTGCCACTGGGCATTGAGCTGCTGTTCCTGCTGCTGCTCGGCCTGCAGGGCAGCCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGT  >  ACB122/3482890‑3483159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑