| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,491,323 | T→G | 51.2% | intergenic (‑685/+36) | CJ019_03306 ← / ← CJ019_03307 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,491,323 | 0 | T | G | 51.2% | ‑2.3 / 9.0 | 25 | intergenic (‑685/+36) | CJ019_03306/CJ019_03307 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/7); new base G (10/2); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCC > ACB122/3491199‑3491453 | cTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGcaca > 8:87821/1‑136 (MQ=255) tACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCCCGCACACCg > 2:339986/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGGCCGCGCACACCCGCGCCGCCggg > 7:52024/1‑135 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCCCGCACACCCCCGCCGCCgg > 5:327310/1‑135 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCCCCCGCCCCCCGACACCCGCgccgcccc > 1:149583/1‑133 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGGACACCCGCGCCGCCgg > 3:42641/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCCCAGCCgg > 4:124180/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCGGGCCGCCGGCCCGGGAACACCCGCGCCGCCgg > 6:321132/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCGGGCCGCCGGCCCCCCCAACCCGGCACAGcccgg > 7:175024/1‑133 (MQ=18) gggcGCCTCGTTGTCGGCGCGGGTTTCCCCGCAAATTGCGCGGCCCTGCCGGCCACTATCCCTGGCCATGCCCCGACCCCCCCCCCCCCGCATGCGCGGGCAGCCGCCCCGCCAACACCGGCGCAGCCGGTTTCa < 4:53660/132‑1 (MQ=255) ttACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACACCCGCGCAGCCCGGTTCATCCAACTCAACCCCCAACGACCt > 2:216770/1‑134 (MQ=21) tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCGGCCCCCGCACACCCCCCCCGCCGCGTTGCTCCCACTCAACGCCCCGCGCCCGGTGCGCCAACgc > 1:263829/1‑134 (MQ=9) tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACACCGGCGCCGCCGGGTTGATACCACTCCACCCCCCACGCCCCGTACGCAAACGt > 5:106149/1‑135 (MQ=18) gCCGGACCTGCCGGCCACTATCCCTGGTCATGCCCCGACACAGCGCCCACCGCATTGGCGGGCAGCCTGCCGGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGtctc < 7:251489/135‑1 (MQ=255) ggACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCGGCCCGCGCACACCCGCGCCGCCGGGTTCATCCAACACAACGACCCTCCCCCGGTACGCCAACGTCTCTGcg > 7:216343/1‑135 (MQ=21) gCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGGCGCCTGCCCCCGAACACCGGCGCAGCCGGTGTACTACCACACCACGCCCATCGCCCGGTACGCCAACGTCTCGGCCATCGATTGc > 6:188142/1‑135 (MQ=25) ccACTATCCCTGGGCATACCCCGTCACAGCCGCCACCGCATCCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCt < 8:52024/136‑1 (MQ=255) cACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCGGGCAGCCTCCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCtt < 7:329438/136‑1 (MQ=255) acagaccccTGGTCTTCCCCCGGCCCCGCCGCCCCCGCATTCGGGGGCCGCCTCCCGGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCt < 3:31446/129‑1 (MQ=255) ttCACCGTCACCCCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGt < 3:238239/135‑1 (MQ=255) cccGCCACCGCCGCCCCCGCATGCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCg < 4:42641/133‑1 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGCCGCCAGCCCGCGAACACCCGCGCAGCCGGGTTGATCCCACACAACCCCCCACCACCGGTACGCCAACCTCTCTGCCCTCGATTTCCATGGATGGCCAAtagctgt > 4:31446/1‑129 (MQ=255) caGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACCTCTCTGCCATCGATTGCCTTGCATGGCCCAATGTGGTTTATCTa > 6:106883/1‑135 (MQ=255) ccccGCATTCGGGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCg < 7:33061/134‑1 (MQ=255) cAGCCGGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc < 8:98465/136‑1 (MQ=255) | CTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCC > ACB122/3491199‑3491453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |