Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,572,268	C→T	6.3%	T33T (ACG→ACA)	CJ019_03385 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,572,268	0	C	T	6.3%	211.8 / 3.2	65	T33T (ACG→ACA)	CJ019_03385	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/25);  new base T (2/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

GTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGCGT  >  ACB122/3572139‑3572399                                                                                                                                  |                                                                                                                                    gttgtCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGaa                                                                                                                                >  3:295327/1‑135 (MQ=255)      cGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGt                                                                                                                            <  3:219319/134‑1 (MQ=255)        ccccTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGttt                                                                                                                          <  2:155513/134‑1 (MQ=255)                      gggggTGGTGTATATTATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGAAGCGCTAGAATTCCTGAGTTTATGAAATTTACTGATTGGATTAGGTATGAACGTGAAATGTTTTGTGGATATTAAttt                                                                                                           >  8:169310/1‑135 (MQ=255)                        gggTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCg                                                                                                        >  8:61563/1‑136 (MQ=255)                           tggtgTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCgg                                                                                                       >  4:201707/1‑134 (MQ=255)                           tggtgTAGATGATTGTTTGTTTGCTGATTATGATAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGa                                                                                                      >  2:48796/1‑135 (MQ=255)                                tAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAAttt                                                                                                 >  2:245951/1‑135 (MQ=255)                                         gtttgtttGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACa                                                                                        >  6:233330/1‑135 (MQ=255)                                           ttgtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAgg                                                                                      >  5:389694/1‑135 (MQ=255)                                           ttgtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAgg                                                                                      >  4:178585/1‑135 (MQ=255)                                           ttgtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAgg                                                                                      >  5:465/1‑135 (MQ=255)                                           ttgtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAgg                                                                                      >  1:231419/1‑135 (MQ=255)                                           ttgtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGt                                                                                     >  7:253748/1‑136 (MQ=255)                                             gtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGtt                                                                                    >  6:84320/1‑135 (MQ=255)                                             gtttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGtt                                                                                    >  6:272105/1‑135 (MQ=255)                                               ttgCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTc                                                                                  <  6:389694/135‑1 (MQ=255)                                                tgctgcTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCg                                                                                 <  7:118891/135‑1 (MQ=255)                                                      ttATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCaa                                                                           <  5:233330/135‑1 (MQ=255)                                                        aTGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAAtct                                                                        >  8:161588/1‑136 (MQ=255)                                                          gCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAAtctc                                                                       <  3:113364/135‑1 (MQ=255)                                                                aaTATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAAc                                                                >  8:155839/1‑136 (MQ=255)                                                                aaTATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAAc                                                                >  7:165637/1‑136 (MQ=255)                                                                  tatTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAAcc                                                               >  5:201210/1‑135 (MQ=255)                                                                  tatTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAAcc                                                               >  6:327757/1‑135 (MQ=255)                                                                       ccTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAATGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAttt                                                         >  7:375516/1‑136 (MQ=255)                                                                       ccTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAATGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAttt                                                         >  7:381333/1‑136 (MQ=255)                                                                       ccTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAtt                                                          >  3:135819/1‑135 (MQ=255)                                                                         tctGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAtttt                                                        >  2:246927/1‑135 (MQ=255)                                                                         tctGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAtttt                                                        <  6:66887/135‑1 (MQ=255)                                                                         tctGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTAtttt                                                        >  2:100952/1‑135 (MQ=255)                                                                            ggCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTcgc                                                     >  3:149749/1‑135 (MQ=255)                                                                              cAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTcgccgc                                                  >  7:83866/1‑136 (MQ=255)                                                                              cAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTcgccg                                                   <  6:33486/135‑1 (MQ=255)                                                                                    tAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTg                                               >  3:251312/1‑133 (MQ=255)                                                                                    tAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTg                                               >  1:74366/1‑133 (MQ=255)                                                                                      gAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAATGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           <  8:381333/135‑1 (MQ=255)                                                                                      gAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAATGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           <  8:375516/135‑1 (MQ=255)                                                                                       aaTTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           <  5:249718/134‑1 (MQ=255)                                                                                       aaTTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTATCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           <  6:189047/134‑1 (MQ=255)                                                                                              gAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCAtt                                  >  8:222037/1‑136 (MQ=255)                                                                                                gTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCAttt                                 <  3:190194/135‑1 (MQ=255)                                                                                                gTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCAttt                                 <  3:178585/135‑1 (MQ=255)                                                                                                           ttACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAAc                     <  8:168951/136‑1 (MQ=255)                                                                                                           ttACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAAc                     <  8:165637/136‑1 (MQ=255)                                                                                                            tACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAAc                     <  6:465/135‑1 (MQ=255)                                                                                                            tACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAAc                     <  4:62346/135‑1 (MQ=255)                                                                                                                      aTTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGc                                                                   <  6:44527/79‑1 (MQ=255)                                                                                                                      aTTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGc                                                                   >  5:44527/1‑79 (MQ=255)                                                                                                                      aTTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGt            >  1:103832/1‑134 (MQ=255)                                                                                                                      aTTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGt            >  2:226603/1‑134 (MQ=255)                                                                                                                       ttAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTaa          <  4:135819/135‑1 (MQ=255)                                                                                                                       ttAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTaa          <  1:100952/135‑1 (MQ=255)                                                                                                                          gggCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAg        <  5:272105/134‑1 (MQ=255)                                                                                                                            gCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           >  2:80141/1‑97 (MQ=255)                                                                                                                            gCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAg                                           <  1:80141/97‑1 (MQ=255)                                                                                                                            gCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAgggg     >  1:178902/1‑135 (MQ=255)                                                                                                                             cTGAAGGTGATATGTTTGGTGGATATTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGc    <  5:9428/135‑1 (MQ=255)                                                                                                                             cTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGc    <  2:74366/135‑1 (MQ=255)                                                                                                                              tGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAg        >  1:101708/1‑130 (MQ=255)                                                                                                                              tGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGc    >  2:382263/1‑134 (MQ=255)                                                                                                                              tGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGCg   >  7:179798/1‑135 (MQ=255)                                                                                                                              tGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGACGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAg        <  2:101708/130‑1 (MQ=255)                                                                                                                                 aCGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGCGt  <  2:95411/133‑1 (MQ=255)                                                                                                                                 aCGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGCGt  <  6:201210/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    GTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAATGTTTGGTGGATAGTAATTTTCGGAAATTTGCGGCGACAGGTTTCGCATCAATCTCGAGCAACCTTATTTTCGCCGCTTGGGAGCTAAGCATTTGAAGTGTCCAACTTTCTACGTAAAGGGGCGT  >  ACB122/3572139‑3572399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑