Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,703,189 T→C 5.4% K89E (AAG→GAG)  paaE ← 1,2‑phenylacetyl‑CoA epoxidase, subunit E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,703,1890TC 5.4% 222.4 / 2.2 72K89E (AAG→GAG) paaE1,2‑phenylacetyl‑CoA epoxidase, subunit E
Reads supporting (aligned to +/- strand):  ref base T (32/36);  new base C (2/2);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CGATGATCGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTCCAGCTGGGTAC  >  ACB122/3703060‑3703320
                                                                                                                                 |                                                                                                                                   
cGATGATCGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCa                                                                                                                                >  4:369348/1‑135 (MQ=255)
     atCGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCa                                                                                                                           >  2:237612/1‑135 (MQ=255)
     atCGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCa                                                                                                                           >  2:317852/1‑135 (MQ=255)
       cGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCAtt                                                                                                                         <  1:392748/135‑1 (MQ=255)
         aCAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGGCCAAAGGCACGAAGACGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTgg                                                                                                                       <  2:258928/135‑1 (MQ=255)
          cAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTgg                                                                                                                       >  5:52721/1‑134 (MQ=255)
                  gggTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCt                                                                                                               >  8:273129/1‑134 (MQ=255)
                  gggTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTGCATTGGCAAACGCt                                                                                                               >  8:273127/1‑134 (MQ=255)
                     tGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACGTCATTGGCAAACGCTgagc                                                                                                           >  5:24499/1‑134 (MQ=255)
                     tGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCATCCGCACTTCATTGGCAAACGCTgaga                                                                                                           >  2:148814/1‑135 (MQ=255)
                        tGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAgc                                                                                                        <  5:162361/135‑1 (MQ=255)
                          ccGCTGCCGGCAGCTGCCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCCGCTGCTGACCGGCCTTGAGCGCTTCATTGGCAAACGCTGAGCAGcgcc                                                                                                     >  7:44740/1‑136 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAACGGCACGAAGACGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGcgcc                                                                                                     >  8:204365/1‑136 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGCAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAgcgc                                                                                                      >  3:104931/1‑135 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAgcgc                                                                                                      >  5:74752/1‑135 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAgcgc                                                                                                      >  3:76933/1‑135 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGcgcc                                                                                                     >  8:209914/1‑136 (MQ=255)
                          ccGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGcgcc                                                                                                     >  7:22220/1‑136 (MQ=255)
                                ccGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCACACGCTGAGAAGCgcccgccc                                                                                                >  1:40974/1‑135 (MQ=255)
                                ccGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCgcccgccc                                                                                                >  3:102373/1‑135 (MQ=255)
                                ccGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTCGAGCACTTCATTGGCAAACGCTGAGAAGCgcccgccc                                                                                                >  6:387872/1‑135 (MQ=255)
                                ccGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTCGAGCACTTCATTGGCAAACGCTGAGAAGCgcccgccc                                                                                                >  6:393255/1‑135 (MQ=255)
                                 cGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCgcccgccc                                                                                                <  3:141128/134‑1 (MQ=255)
                                 cGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGcccgcccg                                                                                               <  4:123343/135‑1 (MQ=255)
                                   gCAGCTACCCCCAGATAGTTGCCCTGGCGATCCTGGTCCAAACGCACGAAGAAGCTACCCGATGGCGGCATCACAGCCAGCGTCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGc                                                                                             <  6:114220/135‑1 (MQ=255)
                                   gCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGc                                                                                             <  6:74752/135‑1 (MQ=255)
                                     aGCTCCCCCGAGATAGTGGCCCTGGCGCGCCGGGTCCAACGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCGGACCGGCCTTGAGCACTTCATTGCCAAACGCTGAGAAGCGCCCGCCCGGCAc                                                                                           <  5:214350/135‑1 (MQ=255)
                                           ccccAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCt                                                                                      <  4:298100/134‑1 (MQ=255)
                                                      tGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCAcgc                                                                          <  3:281607/135‑1 (MQ=255)
                                                        cccTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCAcgcg                                                                         >  8:335449/1‑134 (MQ=255)
                                                        cccTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCAcgc                                                                          >  3:263092/1‑133 (MQ=255)
                                                        cccTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACgcgc                                                                        >  1:41515/1‑135 (MQ=255)
                                                          cgcgCGAGCCGGGTCCAACGGCACGAAGAAGCTCCCCGAGGGCGGCCTCACTGCCAGCTGCGGACCGGCCGTGAGCACCTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTGGACGGCCACGCGCAGc                                                                     <  8:283821/133‑1 (MQ=255)
                                                          cTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGCTGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  8:21874/136‑1 (MQ=255)
                                                          cTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  7:273129/136‑1 (MQ=255)
                                                          cTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  7:273127/136‑1 (MQ=255)
                                                           tGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  1:317852/135‑1 (MQ=255)
                                                           tGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  2:73332/135‑1 (MQ=255)
                                                           tGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  6:154888/135‑1 (MQ=255)
                                                           tGGCGAGCCGGGGCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGc                                                                     <  6:293978/135‑1 (MQ=255)
                                                                aGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGcc                                                                >  3:104090/1‑135 (MQ=255)
                                                                     ggTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGGTGACGGGCCTCGAGCAGTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCt                                                           <  5:393255/135‑1 (MQ=255)
                                                                     ggTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCt                                                           <  1:95993/135‑1 (MQ=255)
                                                                     ggTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTg                                                          >  7:263266/1‑136 (MQ=255)
                                                                     ggTCCAAAGCCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGAGTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCt                                                           <  1:148814/135‑1 (MQ=255)
                                                                     ggTCAAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTCGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCt                                                           <  5:387872/135‑1 (MQ=255)
                                                                       tCCCAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCa                                                        <  7:327838/136‑1 (MQ=255)
                                                                                 cGAAGAAGCTACCCGATGGCTGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCAAGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  5:260226/135‑1 (MQ=255)
                                                                                 cGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCAGTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  3:135901/135‑1 (MQ=255)
                                                                                 cGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  5:135695/135‑1 (MQ=255)
                                                                                 cGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  4:102373/135‑1 (MQ=255)
                                                                                   aagaagCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCt                                                 >  6:210134/1‑131 (MQ=255)
                                                                                    agaagCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGAt                                           >  8:297783/1‑136 (MQ=255)
                                                                                           aCCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTa                                     >  5:304435/1‑135 (MQ=255)
                                                                                           aCCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTa                                     >  3:79288/1‑135 (MQ=255)
                                                                                                   gcggcATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAgcggc                             >  1:181349/1‑135 (MQ=255)
                                                                                                     ggcATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCACACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGAGGGAGTAGGAGCGGCGGa                          <  7:204365/136‑1 (MQ=255)
                                                                                                     ggcATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAACCGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGa                          <  7:209914/136‑1 (MQ=255)
                                                                                                      gcATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGa                          >  1:334347/1‑135 (MQ=255)
                                                                                                         tCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGActt                       <  4:72467/135‑1 (MQ=255)
                                                                                                          cACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCCCGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGActtc                      >  6:68957/1‑135 (MQ=255)
                                                                                                            cTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGActtct                     <  4:104090/134‑1 (MQ=255)
                                                                                                                  gctgctGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTc             <  7:289553/136‑1 (MQ=255)
                                                                                                                  gctgctGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTc             <  8:263266/136‑1 (MQ=255)
                                                                                                                   ctgctgACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTc             <  6:52721/135‑1 (MQ=255)
                                                                                                                    tgctgACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  6:339533/100‑1 (MQ=255)
                                                                                                                    tgctgACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               >  5:339533/1‑100 (MQ=255)
                                                                                                                          ccGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGCCTTCTTCGTTGTCCAGCTgg      >  1:265641/1‑135 (MQ=255)
                                                                                                                          ccGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTCCAGCt        <  1:37255/133‑1 (MQ=255)
                                                                                                                            ggCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               <  7:245587/92‑1 (MQ=255)
                                                                                                                            ggCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGc                                               >  8:245587/1‑92 (MQ=255)
                                                                                                                              ccTTGAGCACTTCATTGGCAACCGCGGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTCCAGCTGGGTAc  <  6:24499/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
CGATGATCGACAGTATCGGGGTGATGCCGCTGCCGGCAGCTACCCCCAGATAGTTGCCCTGGCGAGCCGGGTCCAAAGGCACGAAGAAGCTACCCGATGGCGGCATCACTTCCAGCTGCTGACCGGCCTTGAGCACTTCATTGGCAAACGCTGAGAAGCGCCCGCCCGGCACGCGCTTGACGGCCACGCGCAGCTCGCCGTCCTGCACGGCGCTGCAGATGGAGTAGGAGCGGCGGACTTCTTCGTTGTCCAGCTGGGTAC  >  ACB122/3703060‑3703320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑