Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,851,026	C→T	5.6%	R145R (CGC→CGT)	CJ019_03648 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,851,026	0	C	T	5.6%	234.7 / 3.6	71	R145R (CGC→CGT)	CJ019_03648	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/37);  new base T (2/2);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GCACATTGCCCGCGAAGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACGCTGTA  >  ACB122/3850900‑3851159                                                                                                                               |                                                                                                                                      gcaCATTGCCCGCGAAGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCt                                                                                                                               <  1:12519/135‑1 (MQ=255)        tcccGCGAAGCGGTGGCGAACAACTGGTGGCGCCTGGAATGGTGTGTGCTGGACTGCAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGc                                                                                                                        <  7:257239/134‑1 (MQ=255)         cccGCGAAGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGCAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCg                                                                                                                       >  1:346679/1‑135 (MQ=255)          ccGCGAAGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGACACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCg                                                                                                                       >  2:285200/1‑134 (MQ=255)               aaGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGc                                                                                                                 >  4:67065/1‑135 (MQ=255)                aGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGTTACCGGCTGGATGGCGACAAGCt                                                                                                                >  3:75187/1‑135 (MQ=255)                aGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGTTACCGGCTGGATGGCGACAAGCt                                                                                                                >  3:69866/1‑135 (MQ=255)                             aacTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGcc                                                                                                   <  4:175945/135‑1 (MQ=255)                                  tgGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCgg                                                                                              >  6:356340/1‑135 (MQ=255)                                    ggCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCggg                                                                                             >  4:363382/1‑134 (MQ=255)                                     gcgcCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTg                                                                                           >  4:218905/1‑135 (MQ=255)                                       gcCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGTGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGAt                                                                                         >  1:246544/1‑135 (MQ=255)                                       gcCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGAt                                                                                         >  5:250923/1‑135 (MQ=255)                                         cTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCg                                                                                       <  4:188289/135‑1 (MQ=255)                                          tGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGcc                                                                                     >  7:97124/1‑136 (MQ=255)                                          tGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGc                                                                                      >  2:1397/1‑135 (MQ=255)                                                      tgCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCa                                                                          >  1:358370/1‑135 (MQ=255)                                                         tggactggaACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACtt                                                                       >  1:298711/1‑135 (MQ=255)                                                         tggactggaACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACtt                                                                       >  6:24370/1‑135 (MQ=255)                                                         tggactggaACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACtt                                                                       >  1:301279/1‑135 (MQ=255)                                                         tggactggaACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACtt                                                                       >  1:314901/1‑135 (MQ=255)                                                             ctggaACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTctgc                                                                   <  5:24370/135‑1 (MQ=255)                                                                 aaCGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTctgcctgc                                                               <  1:55685/135‑1 (MQ=255)                                                                   cGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCctgctg                                                             >  8:272312/1‑135 (MQ=255)                                                                   cGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCctgct                                                              >  1:32342/1‑134 (MQ=255)                                                                   cGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCctgct                                                              >  3:82422/1‑134 (MQ=255)                                                                   cGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCctgct                                                              >  2:233169/1‑134 (MQ=255)                                                                   cGAGCCAGCGATCGGCTTCTACCAGAAACTGGGCGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGCCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCctgct                                                              >  1:350536/1‑134 (MQ=255)                                                                     aGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGgaa                                                          <  8:354475/136‑1 (MQ=255)                                                                      gCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGgaa                                                          <  4:353614/135‑1 (MQ=255)                                                                      gCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGgaa                                                          <  5:243222/135‑1 (MQ=255)                                                                        cAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTgcgc                                                                                                                                       >  1:52955/1‑56 (MQ=255)                                                                        cAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTgcgc                                                                                                                                       <  2:52955/56‑1 (MQ=255)                                                                        cAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGgaa                                                          >  4:85726/1‑133 (MQ=255)                                                                        cAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCACGGTTTCTGCACTTCTGCCTGCTGgaa                                                          >  6:378672/1‑133 (MQ=255)                                                                        cAGCGATCGGCTTCTACAAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACACGCTGGCGGCATTCGCCCTCGGGTGATCGCCAGGTTTCTGCACTTCTGCCTGCTGgat                                                          >  6:216005/1‑132 (MQ=255)                                                                           cGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGAGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAagaaga                                                     <  6:105608/135‑1 (MQ=255)                                                                           agATCGGCTTCTACCAGAAACTGGGGTCCGATGCGCAGGATGAGTGGTTGCGCTACCGTCTGGATGGCGACAAGCTGGCGGCATTCTCCCGAGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTTGAagaaga                                                     <  6:99978/134‑1 (MQ=255)                                                                            gATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAagaaga                                                     <  3:85726/134‑1 (MQ=255)                                                                             aTCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACt                                                   >  6:116938/1‑135 (MQ=255)                                                                                 gCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAg                                               <  4:363886/135‑1 (MQ=255)                                                                                  cTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAgg                                              >  3:125534/1‑135 (MQ=255)                                                                                  cTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAgg                                              >  3:385029/1‑135 (MQ=255)                                                                                   ttCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGc                                             >  2:160080/1‑135 (MQ=255)                                                                                   ttCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGc                                             >  6:42772/1‑135 (MQ=255)                                                                                      tACCAGATACTGGGGGCCGAGGCGGAGGATGAGTGGGTGCGCTACCGGCGGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCt                                           <  4:333504/134‑1 (MQ=255)                                                                                         cAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAg                                        <  2:298711/134‑1 (MQ=255)                                                                                          aGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTTGAAGAAGACTTCAGGCCTGAGcc                                      <  5:37599/135‑1 (MQ=255)                                                                                          aGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGcc                                      <  2:32342/135‑1 (MQ=255)                                                                                               cTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGt                                <  8:38060/136‑1 (MQ=255)                                                                                                tGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGt                                <  1:359591/135‑1 (MQ=255)                                                                                                     gCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACctg                           >  3:75278/1‑135 (MQ=255)                                                                                                          ggCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGtt                      <  4:82422/135‑1 (MQ=255)                                                                                                          ggCGCAGGATGAGGGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGtt                      <  6:258806/135‑1 (MQ=255)                                                                                                           gcgcAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCg                    <  8:24598/136‑1 (MQ=255)                                                                                                            cgcAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCg                    <  3:367561/135‑1 (MQ=255)                                                                                                                  aggcgTGGGTGCGCGACCGGCTGGATGGCGACTAGCTGGCGGCATTCGCCCGCGGGTGAGCGCCCGGGTTCTGCACTTCTGCCTGCGGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGcc              <  4:249940/131‑1 (MQ=255)                                                                                                                  aTGAGTGGGTGCGCTA‑CGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  7:1816/136‑1 (MQ=255)                                                                                                                   tGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  7:257844/136‑1 (MQ=255)                                                                                                                   tGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  7:359161/136‑1 (MQ=255)                                                                                                                   tGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  8:97124/136‑1 (MQ=255)                                                                                                                    gAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  6:275306/135‑1 (MQ=255)                                                                                                                    gAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  4:41037/135‑1 (MQ=255)                                                                                                                    gAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  3:195452/135‑1 (MQ=255)                                                                                                                    gAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  3:166268/135‑1 (MQ=255)                                                                                                                     aGTGGGTGCGTTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  4:75187/134‑1 (MQ=255)                                                                                                                     aGTGGGTGCGTTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGGTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTc            <  4:69866/134‑1 (MQ=255)                                                                                                                     aGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGc          <  7:349039/136‑1 (MQ=255)                                                                                                                      gTGGGTGCGCTTCCGGCTGGATGGCGACTAGCTGGCGGCATTCGGCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCGTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGAGAGCCTGGc          <  2:350536/135‑1 (MQ=255)                                                                                                                      gTGGGTGCGCTACCGGCTGGATTGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGc          <  4:328680/135‑1 (MQ=255)                                                                                                                      gTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGc          <  3:360656/135‑1 (MQ=255)                                                                                                                      gTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGc          <  2:392342/135‑1 (MQ=255)                                                                                                                         ggTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACg       >  2:389881/1‑135 (MQ=255)                                                                                                                            gcgcTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACGCt     >  2:126998/1‑134 (MQ=255)                                                                                                                              gcTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGTCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACGCTGTa  >  1:267752/1‑135 (MQ=255)                                                                                                                              gcTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACGCTGTa  >  3:183295/1‑135 (MQ=255)                                                                                                                               |                                                                                                                                      GCACATTGCCCGCGAAGCGGTGGCGAACAACTGTGGGCGCCTGGAATGGAGTGTGCTGGACTGGAACGAGCCAGCGATCGGCTTCTACCAGAAACTGGGGGCCGAGGCGCAGGATGAGTGGGTGCGCTACCGGCTGGATGGCGACAAGCTGGCGGCATTCGCCCGCGGGTGATCGCCCGGTTTCTGCACTTCTGCCTGCTGGAAGAAGACTTCAGGCCTGAGCCTGTCGTACCTGCTGTTCGACAGCCTCGCACGCTGTA  >  ACB122/3850900‑3851159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑