Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,875,885 A→C 23.4% N300T (AAC→ACC)  ttgI_2 → Toluene efflux pump outer membrane protein TtgI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,875,8850AC23.4% 65.1 / 2.6 45N300T (AAC→ACC) ttgI_2Toluene efflux pump outer membrane protein TtgI
Reads supporting (aligned to +/- strand):  ref base A (11/22);  new base C (7/5);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTG  >  ACB122/3875755‑3876017
                                                                                                                                   |                                                                                                                                    
aGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCCAcctg                                                                                                                                  >  2:347486/1‑132 (MQ=255)
aGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCCACgat                                                                                                                                  >  4:111662/1‑133 (MQ=255)
     aGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCACCGGTGGcct                                                                                                                             >  5:133689/1‑133 (MQ=255)
             cGCCCACGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCAc                                                                                                                       <  2:88760/133‑1 (MQ=255)
               cccACGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCAccgcc                                                                                                                   <  3:84721/135‑1 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGGCCCCCCCCACCGTGGGGGTGGCcccccc                                                                                                                    >  2:294106/1‑133 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCGCCCACGGTGGCGGTGCCAccccc                                                                                                                   >  3:120859/1‑135 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCGCCCACCTTGGCGTGGcccccccc                                                                                                                   >  6:393278/1‑135 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCCCCACCCGTGGCGTGGcccacccc                                                                                                                   >  4:567/1‑129 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCCACGTTGGCGTGGGCAccgccg                                                                                                                  >  8:37214/1‑136 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCACCGTTGGCGTGGGCAccgccg                                                                                                                  >  7:35972/1‑136 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCAACGTTGGCGTGGCCAccgccg                                                                                                                  >  7:42866/1‑136 (MQ=255)
               cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCCACCGTGGCGTCGCCCccgccc                                                                                                                  >  7:157404/1‑135 (MQ=255)
                      cccTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCACCGTTGGCGGGGCCCCCGCCGcccggg                                                                                                            >  3:54220/1‑132 (MQ=255)
                        cTGCCGGCCATCGCCAAGGCGCTGCCGGGCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTc                                                                                                          <  6:177783/135‑1 (MQ=255)
                           ccGGCCAGCGCCACGGCGCGGCCGGGCGGGGACCCGGGGGAGCTGCGGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCcg                                                                                                       <  6:390770/135‑1 (MQ=255)
                              gCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGGGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGGGGCCACCGCCGACCTGTTCCcgcgc                                                                                                    <  4:138070/135‑1 (MQ=255)
                              gCCAGCGCCAAGGCGGTGCGGGGCGGTGACCCGGGTGAGCGGCGGCGCCGCCGCCCGGACACCGGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTGGGCGTGGCCACCGCCGACCTGTTCCcgcgc                                                                                                    <  6:214530/135‑1 (MQ=255)
                                            gcTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCCACCTTGGCCAGGGCCACGGCGACCAGCTGCCCCCCCTCCGCCACAGTGGc                                                                                     >  8:86284/1‑136 (MQ=255)
                                            gcTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGc                                                                                     <  8:245793/136‑1 (MQ=255)
                                                ccGGGCGGGGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTc                                                                                  <  1:101900/135‑1 (MQ=255)
                                                           cccGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCCCCCTTGGCGTGGGCCCCCCCCCCCGGTTCCCCCCCGGCAGCCTCACTGGGGTGCTCGGCgttta                                                                       >  2:353263/1‑133 (MQ=255)
                                                                 tGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCCACGTTGGCGTGGCCCCCGCCGCCCGGTTCCCGCGCGTCCGCCGCAGTGGCGTGCTCGGCTTTACCgccgcc                                                                >  7:104582/1‑136 (MQ=255)
                                                                  gAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGTGGC‑GGCCCCCGCCCACGTTGGCGTGGCCACCGCCGCCCTGTTCCCGCGCGTCAGCCTCCGTTGCGTCCTCGGCTGTACCgccgcc                                                                >  3:271217/1‑135 (MQ=255)
                                                                  gAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCCCCGTTGGCGGGGCCCCCGCCCGCCTGGTGCCGCCCGGCAGGCTCAGGGGGTGCCGAGGCTGTTCCgccgcc                                                                >  5:225445/1‑135 (MQ=255)
                                                                     cgcggccgcggccgcCCGGACCGCCGCCCCCCGGAGCGCCCGCGGGCGGC‑GCCCACCGCCTCGGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCGCAGTGGCTTCCTCGGCTTTACCGCCGCCcgcg                                                            <  7:142517/130‑1 (MQ=255)
                                                                             ccgccgccCGGACAGCCGCTGCGCCGAGCGCCAGCGGGCGGC‑GGCCACCGCCAAGGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCa                                                     <  5:392111/135‑1 (MQ=255)
                                                                                ccgccCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTGGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGAt                                                  <  1:294106/135‑1 (MQ=255)
                                                                                     cGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCAACGTTGGCGTGGCCACCGCCGCCCGGTTCCCGCGCGTCCGCCGCAGTGGCGTCCTCGGGTTTTCCGCCGCCCGCGGCGCGCCGATCGGctc                                            >  8:12030/1‑136 (MQ=255)
                                                                                        acccccgccgCGCCGCGCGCCCGCTGGCGGC‑GCCCCCCGCAACCGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCtt                                          <  5:249534/131‑1 (MQ=255)
                                                                                        aCAGCCGCAGCGCCGAGCGCCCGCGGGCGGC‑GGCCACCGCCACCGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCtt                                          <  1:91722/135‑1 (MQ=255)
                                                                                            ccGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcg                                     <  7:140464/136‑1 (MQ=255)
                                                                                             cGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGGGGCCACCGCCGACCTGTTCCCGCGCGGCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcg                                     <  2:286069/135‑1 (MQ=255)
                                                                                              gCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcgc                                    <  3:288920/135‑2 (MQ=255)
                                                                                               ccgcgcCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCggcggc                                   <  1:270049/133‑1 (MQ=255)
                                                                                                     cGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc                             <  4:291666/135‑1 (MQ=255)
                                                                                                                gggcggg‑ggcCACCGCCACCGGGGGCGGGCCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg                  <  5:331939/134‑1 (MQ=255)
                                                                                                                gggcggc‑ggcCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg                  <  5:106328/134‑1 (MQ=255)
                                                                                                                 ggcggc‑ggcCACCGCCACCGTGGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTggg                 <  5:390274/135‑1 (MQ=255)
                                                                                                                    ggc‑ggcCACCGCCAACGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTCCCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCg             <  8:90763/136‑1 (MQ=255)
                                                                                                                     gggggcCACCGCCAACGTGGGCGGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGccc              <  3:567/132‑1 (MQ=255)
                                                                                                                     gc‑ggcCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGa            <  7:18200/136‑1 (MQ=255)
                                                                                                                               gCCAAAGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTGAGTGGCTTCCTCGGCTTTACGGCCGCCCGCTGCTAGCAGATCGGCTCTTCGGCGGCCAACGCCTTGGCACTGGGCCCGAGCATTACCt   <  7:86284/136‑1 (MQ=255)
                                                                                                                                ccAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCt   <  4:271217/135‑1 (MQ=255)
                                                                                                                                 cgaCGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg  <  6:202127/133‑1 (MQ=255)
                                                                                                                                 cAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg  <  6:379617/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
AGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTG  >  ACB122/3875755‑3876017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑