Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,931,996	A→G	5.0%	D118G (GAT→GGT)	CJ019_03714 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,931,996	0	A	G	5.0%	262.9 / 3.7	81	D118G (GAT→GGT)	CJ019_03714	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/60);  new base G (2/2);  total (18/63)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

CAACTTTGTTGATCCAAGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCATTTCGGCTAGAAGC  >  ACB122/3931862‑3932126                                                                                                                                       |                                                                                                                                   cAACTTTGTTGATCCAAGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTtgatga                                                                                                                                    >  3:239640/1‑135 (MQ=255)        gttgATCCAAGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACa                                                                                                                               <  6:46196/133‑1 (MQ=255)               cAAGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTAtt                                                                                                                     <  7:131208/136‑1 (MQ=255)                aaGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTAtt                                                                                                                     <  3:232730/135‑1 (MQ=255)                aaGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTAtt                                                                                                                     <  2:217711/135‑1 (MQ=255)                    ggCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTaaa                                                                                                                  >  5:225931/1‑134 (MQ=255)                    ggCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTaaa                                                                                                                  >  4:341184/1‑134 (MQ=255)                      ccATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAaacaa                                                                                                               <  2:228358/135‑1 (MQ=255)                      ccATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAaacaa                                                                                                               <  3:341184/135‑1 (MQ=255)                      ccATTCCGGCGTTCTGACACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAaacaa                                                                                                               <  5:3261/135‑1 (MQ=255)                            cgtcGTTCTTCAACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATTATTACATAAAAATATTAAACAACAAAAAg                                                                                                        <  8:189556/133‑1 (MQ=255)                            cgtcGTTCTGCCACTTCAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTAATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  8:253722/133‑1 (MQ=255)                            cGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGTCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  7:17304/136‑1 (MQ=255)                            cGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  8:67541/136‑1 (MQ=255)                            cGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  7:134741/136‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  1:289073/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  5:85303/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  5:192489/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  3:16732/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  3:185626/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  2:234268/135‑1 (MQ=255)                             ggCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATAAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  3:228264/135‑1 (MQ=255)                             ggCGTGCTGCCACTAAAAATTCAGGCCGCGCAAAAATTACCGCCTTCGGCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCAAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  1:395779/135‑1 (MQ=255)                               cGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCACAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCa                                                                                                     <  7:33207/136‑1 (MQ=255)                               cGTTCTACCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCa                                                                                                     <  8:390829/136‑1 (MQ=255)                                gTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCa                                                                                                     <  1:81780/135‑1 (MQ=255)                                gTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCACAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCa                                                                                                     <  6:384916/135‑1 (MQ=255)                                gTTCTGCCACTAAAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCATTTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCa                                                                                                     <  5:327273/135‑1 (MQ=255)                                  tCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGTGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCaaa                                                                                                   <  3:308772/135‑1 (MQ=255)                                      ccACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAAcc                                                                                                 <  8:350015/133‑1 (MQ=255)                                      ccACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCt                                                                                                <  8:174888/134‑1 (MQ=255)                                       cACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCt                                                                                                <  6:137575/133‑1 (MQ=255)                                       cACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCt                                                                                                <  4:239640/133‑1 (MQ=255)                                       cACTACAAATTCTGGCCGCCCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGAATCCAACATGCGGGCATCCCAATTAATGTTTGATTATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGt                                                                                              <  4:12753/135‑1 (MQ=255)                                       cACTACAAATTCTGGCAGCGCAAAAATTAACGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGCTCATACCAAATAATGTATGATGATTACATAAAACTATTAAACAAAAAAAAGCCACACCTGt                                                                                              >  5:48871/1‑135 (MQ=255)                                       cACTACAAATTCTGGACGCGAAAAAATTACAGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATTATTACATAAAACTATTAAACAACAAAAAGCCAAACCt                                                                                                <  2:262388/133‑1 (MQ=255)                                              aaTTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGTAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTATTGTTTGATGATTACATAAAAATATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  2:166855/135‑1 (MQ=255)                                              aaTTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  1:327799/135‑1 (MQ=255)                                              aaTTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  5:398526/135‑1 (MQ=255)                                              aaTTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  6:154559/135‑1 (MQ=255)                                                      ccGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGActc                                                                               <  3:351909/135‑1 (MQ=255)                                                       cgcgCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       >  8:29307/1‑126 (MQ=255)                                                       cgcgCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  7:29307/126‑1 (MQ=255)                                                       cgcgCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACtctc                                                                             <  7:116317/136‑1 (MQ=255)                                                       cgcgCAAAAATTACAGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATACAACCAGCGGTCATCCCAAATAATCTATGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTAAACCATGGAGActct                                                                              >  1:87893/1‑135 (MQ=255)                                                        gcgcAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATTTTTTATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACtctc                                                                             <  4:245220/135‑1 (MQ=255)                                                        gcgcAAAAATTACCGCCTTCGTCAAGGACAGTAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACtctc                                                                             <  5:156001/135‑1 (MQ=255)                                                          gcAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCga                                                                           <  6:225931/135‑1 (MQ=255)                                                              aaaTTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGata                                                                       >  5:371057/1‑135 (MQ=255)                                                                 ttACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTc                                                                    <  5:371705/135‑1 (MQ=255)                                                                     cGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAgg                                                                <  4:210608/135‑1 (MQ=255)                                                                         ttCGTCAAGGCCAGGATATTTAATAGCATCCACCCTGCTGTCATCCCAATTAATGTTTGATGATTACTTAAAACTATTAAACCACAAAATGCCATACATGTATACCATGGAGACTCTCGAGATATTCAAGGGAcc                                                            <  6:60180/135‑1 (MQ=255)                                                                         ttCGTCAAGGCCAGGAAATTTAAGATCATCCTACCTGCGCTGATCCCAATTAATGTTTTATGTTTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATAGCATGGAGACTCTCGAGATATTCAAGGGAcc                                                            <  6:6910/135‑1 (MQ=255)                                                                         ttCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGAcc                                                            <  5:262638/135‑1 (MQ=255)                                                                         ttCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGAcc                                                            <  5:332492/135‑1 (MQ=255)                                                                             tCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACcacaca                                                       <  8:193215/136‑1 (MQ=255)                                                                              cAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGCTGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACcacaca                                                       <  4:43582/135‑1 (MQ=255)                                                                               aaGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTAATCCCAATTTATGTTTGATGATTAAATAAAACTTTTAAAAAACAAAAAGCCAAACCTGTATACGATGGAGACTCTCGAGATATTCGAGGGACcactca                                                       <  3:38004/134‑1 (MQ=255)                                                                                    cAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAAGCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGata                                                 <  5:74868/135‑1 (MQ=255)                                                                                    cAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGata                                                 <  2:43718/135‑1 (MQ=255)                                                                                         aaTTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  8:2159/75‑1 (MQ=255)                                                                                         aaTTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        >  7:2159/1‑75 (MQ=255)                                                                                              aaGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        >  6:334482/1‑70 (MQ=255)                                                                                              aaGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAg                                                                                                        <  5:334482/70‑1 (MQ=255)                                                                                                   cATCAAAACTGCGTTCAACCCAATTTATGTTTGATAATAACATAAAACTATTAAAAAACAAAAAGCAAAACCTGTATACCATTGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGTGAGt                                  <  2:237009/135‑1 (MQ=255)                                                                                                       cAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAACACGCCAAACCAGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGc                              <  8:362275/135‑1 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGGTTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  5:136090/77‑1 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGGTTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       <  5:136096/77‑1 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGGTTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       >  6:136090/1‑77 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGGTTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCAt                                                                                       >  6:136096/1‑77 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCa                             >  4:223871/1‑135 (MQ=255)                                                                                                        aaCCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATAATGATCCGAGAGTCGGCAc                            >  8:389717/1‑136 (MQ=255)                                                                                                               ggTCATCGCAATTTATGTTTGATGATTACATAAAACTATTTATATACAAAAAGCCAAAACTTTTTACCATGGAGACTCTCGTGATATTCAAGGGACCACACACTGATTTCATGATCCTAGAGTCGGCACGTGAt                       <  2:87893/134‑1 (MQ=255)                                                                                                                 tCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCt                    >  5:379559/1‑135 (MQ=255)                                                                                                                      ccAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGatat                                                                      <  2:34178/80‑1 (MQ=255)                                                                                                                      ccAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGatat                                                                      >  1:34178/1‑80 (MQ=255)                                                                                                                       cAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCAttt             >  7:236476/1‑136 (MQ=255)                                                                                                                       cAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCAtt              >  2:340137/1‑135 (MQ=255)                                                                                                                           tAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAgg                                                                >  7:98173/1‑81 (MQ=255)                                                                                                                           tAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAgg                                                                <  8:98173/81‑1 (MQ=255)                                                                                                                             aTGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCATTTCgg          >  4:189276/1‑133 (MQ=255)                                                                                                                                 ttGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCATTTCGGCTAGaa    <  3:189276/135‑1 (MQ=255)                                                                                                                                     tgatTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCATTTCGGCTAGAAGc  >  1:82803/1‑133 (MQ=255)                                                                                                                                       |                                                                                                                                   CAACTTTGTTGATCCAAGTGGCCATTCCGGCGTTCTGCCACTACAAATTCTGGCCGCGCAAAAATTACCGCCTTCGTCAAGGCCAGGAAATTTAAGAGCATCCAACCTGCGGTCATCCCAATTAATGTTTGATGATTACATAAAACTATTAAACAACAAAAAGCCAAACCTGTATACCATGGAGACTCTCGAGATATTCAAGGGACCACACACAGATATCATGATCCGAGAGTCGGCACGTGATGCTTTCATTTCGGCTAGAAGC  >  ACB122/3931862‑3932126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑