Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,957,240 C→T 100% G137R (GGG→AGG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,957,2400CT98.3% 266.0 / ‑6.2 82G137R (GGG→AGG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (43/35);  minor base G (1/2);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGC  >  ACB122/3957109‑3957367
                                                                                                                                   |                                                                                                                               
gcgcTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTc                                                                                                                              <  5:138890/135‑1 (MQ=255)
 cgcTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCg                                                                                                                             <  5:340868/135‑1 (MQ=255)
   cTCCGGTAGACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCTGCGCACCGTCCACGGTGATCACCACGTCTTGCCTTTCGGc                                                                                                                           <  5:368653/135‑1 (MQ=255)
      cGGTACATCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGc                                                                                                                       >  7:221760/1‑136 (MQ=255)
       ggTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGc                                                                                                                       >  3:186965/1‑135 (MQ=255)
       ggTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      <  8:11983/136‑1 (MQ=255)
        ggacacCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      <  4:238603/133‑1 (MQ=255)
        gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      <  3:377019/135‑1 (MQ=255)
         tACACCGTGGGGGTCCAGGTAGCCGGGGGCCTCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCggg                                                                                                                    <  7:111888/136‑1 (MQ=255)
             ccGTGGGTGGCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCgg                                                                                                                <  7:252349/136‑1 (MQ=255)
                tGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCgggg                                                                                                              <  5:85388/135‑1 (MQ=255)
                 gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                             >  2:296320/1‑135 (MQ=255)
                 gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                             >  3:195455/1‑135 (MQ=255)
                 gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                             >  6:91928/1‑135 (MQ=255)
                 gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                             >  3:185002/1‑135 (MQ=255)
                 gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCGGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCCCGTCCTGCCGTTCGGCCCGCGGGTCggggg                                                                                                             >  1:339416/1‑134 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGCATCCGGCAGCGCACCGTCAACGGTGATCACCAACTCATCCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  4:386277/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCCCGGTGATCACCACGTCATCCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  3:20200/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  1:78864/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  4:100914/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  5:205609/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  1:243998/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCACCGGTGCTCACCACGTCATGCCTGTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  5:262985/1‑135 (MQ=255)
                   gtgtCCAGGTAGCCGGGGGCAGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  4:109573/1‑135 (MQ=255)
                     gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGATAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGCTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTgcg                                                                                                         >  1:379923/1‑135 (MQ=255)
                      tCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGATAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCt                                                                                                       >  7:172948/1‑136 (MQ=255)
                        cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                      <  3:27770/135‑1 (MQ=255)
                         aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCg                                                                                                    >  8:233129/1‑136 (MQ=255)
                           gTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTAGGCCAGCGGGTCGGGGTTGCGCTGCg                                                                                                    >  3:212393/1‑134 (MQ=255)
                           gTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCAAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCAACACGTCATGCCTTTCGGCCCGCGGGTCGGGGTTGCGCTGCg                                                                                                    >  4:115429/1‑134 (MQ=255)
                                    ggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                         <  7:117878/136‑1 (MQ=255)
                                          gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                    <  3:160397/135‑1 (MQ=255)
                                            aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGt                                                                                             >  5:166030/1‑124 (MQ=255)
                                            aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGt                                                                                             <  6:166030/124‑1 (MQ=255)
                                            aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGCTcac                                                                                 >  7:87122/1‑136 (MQ=255)
                                            aGGGGATGCTGAAAATATTGCACAGCGGCACAGCGATCAGTTGCGAATCCCGCAGCGCACCGTCCACGGTGCTCACCACGACCTGCCTTTCGGCCACCGGGTAGGGGTTGCCCAGCGACACCGTCAGCTCGCTca                                                                                  >  2:286057/1‑135 (MQ=255)
                                             ggggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacc                                                                                <  7:55631/136‑1 (MQ=255)
                                               tgATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                               <  1:201670/134‑1 (MQ=255)
                                                  tGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACtt                                                                            >  6:287280/1‑135 (MQ=255)
                                                  tGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACtt                                                                            >  6:282093/1‑135 (MQ=255)
                                                     tGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                         <  4:251559/135‑1 (MQ=255)
                                                     tGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                         <  3:18625/135‑1 (MQ=255)
                                                      gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCCCCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                        >  5:66786/1‑135 (MQ=255)
                                                      gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCACGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                        >  3:190497/1‑135 (MQ=255)
                                                        aaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                     <  8:198224/136‑1 (MQ=255)
                                                        aaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                     <  8:221760/136‑1 (MQ=255)
                                                         aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                     <  6:205609/135‑1 (MQ=255)
                                                           tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaaa                                                                   <  5:91928/135‑1 (MQ=255)
                                                             ttGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGc                                                                 >  6:97183/1‑135 (MQ=255)
                                                                 ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGCCAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgt                                                             >  4:93416/1‑135 (MQ=255)
                                                                      ggCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCGGCGCCAGCGTCAGCTCGATCCCCACTTCCGGGGAAAGCATGTTGTAg                                                        >  2:384262/1‑135 (MQ=255)
                                                                        cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      >  8:77611/1‑71 (MQ=255)
                                                                        cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      <  7:77611/71‑1 (MQ=255)
                                                                                  aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                            >  1:324677/1‑135 (MQ=255)
                                                                                    ttGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                          <  6:66786/135‑1 (MQ=255)
                                                                                       cGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCAt                                       >  8:312536/1‑135 (MQ=255)
                                                                                         aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  7:343410/136‑1 (MQ=255)
                                                                                         aaTCCGCCAGCGCACCGTCCACGGTGATCACCACGTCATGCCGTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  7:87146/136‑1 (MQ=255)
                                                                                          aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCa                                     >  2:365519/1‑134 (MQ=255)
                                                                                          aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  3:393736/135‑1 (MQ=255)
                                                                                          aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  2:324677/135‑1 (MQ=255)
                                                                                            ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                  <  1:365519/135‑1 (MQ=255)
                                                                                                   cgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGTGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                           <  4:81308/135‑1 (MQ=255)
                                                                                                   cgcACCGTCCACGGTGATCACCACGGCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                           <  5:37219/135‑1 (MQ=255)
                                                                                                             aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                 <  4:365295/135‑1 (MQ=255)
                                                                                                             aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                 >  4:199968/1‑135 (MQ=255)
                                                                                                             aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGGGc                 <  4:186965/135‑1 (MQ=255)
                                                                                                              cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCACCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                <  1:32669/135‑1 (MQ=255)
                                                                                                              cGGTGATCACCACGTCAGGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                <  4:190497/135‑1 (MQ=255)
                                                                                                              cGGTGATCACCACGCCATGCCGTTCGGCCAGAGGGTCGGGGTGGCGCTGCGACAGCGGCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                <  2:168119/135‑1 (MQ=255)
                                                                                                                gTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGTCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCgg               >  6:192747/1‑134 (MQ=255)
                                                                                                                     caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    >  3:277777/1‑108 (MQ=255)
                                                                                                                     caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  4:277777/108‑1 (MQ=255)
                                                                                                                     caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg         >  7:55865/1‑135 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCATCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCa       >  7:221510/1‑135 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCATCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCa       >  7:215878/1‑135 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc        >  3:106317/1‑134 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCa       >  7:151435/1‑135 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCa       >  8:334302/1‑135 (MQ=255)
                                                                                                                       ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCGCGATCCCCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGCCATCAGCCACCGACCCATGCCCTGCgtgcagtgca       >  2:178857/1‑135 (MQ=255)
                                                                                                                           gTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc  <  8:264495/136‑1 (MQ=255)
                                                                                                                            tCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc  >  1:202869/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                               
GCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGC  >  ACB122/3957109‑3957367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑