| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,977,481 | A→C | 44.5% | T284P (ACC→CCC) | ilvE → | Branched‑chain‑amino‑acid aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,977,481 | 0 | A | C | 44.5% | 10.6 / 3.0 | 30 | T284P (ACC→CCC) | ilvE | Branched‑chain‑amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (11/4); new base C (11/3); total (23/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGT > ACB122/3977352‑3977614 | tCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGCCCGTGATCTAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGGCATCGGCCCcgcg > 5:323929/1‑135 (MQ=255) aCTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTGCGTCGAAGCCCGTGCCTCCCGCCCCGCGGGCga > 5:64549/1‑134 (MQ=255) aTCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCTCCGCGGCCGTTCTCAcccc > 7:379638/1‑134 (MQ=255) cACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCCCCCCgct > 5:87036/1‑133 (MQ=255) cACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGGTATCCCCCCGAt > 4:43673/1‑135 (MQ=255) cACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCCGTGCCGGCGGCCCCGCGGCCGTGTTGACCCCCAt > 6:340518/1‑135 (MQ=255) ccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGGTATAAccccccgcgg > 5:396531/1‑129 (MQ=255) ctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCAt > 3:134953/1‑135 (MQ=255) cTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCGGCGGCACCGCGGCCGGTTTGACCCCCAACggcggccgcga > 3:216685/1‑129 (MQ=255) gcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATAACCCCGCTCGGCGGCATGGCGTACCACGGCCAGCt > 1:121926/1‑135 (MQ=255) gcgcCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGGCGTTATAACCCCGCTCGGGGGGATCGCGTACCACGGCCAGCTGCAccg > 2:326410/1‑133 (MQ=255) gcgcCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGCCGTTATCACCCCGCTCGGCGGCCTCGCGTACAACGGCAAGCTGcccctg > 8:232262/1‑131 (MQ=255) cGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCCGTGCCTGCGGCCCCCCGGCCGGTATAACCCCGCTCGGCGGCAAAGAGTAAAAAAGCCAGCTGCAAGTGTTACACGCACTgg > 3:116136/1‑134 (MQ=255) tCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAgg > 3:183843/1‑135 (MQ=255) tCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACCGCCAGCTGCACGTGTTCCACCAACTGGAAAAgg > 2:19251/1‑135 (MQ=255) tCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCCGTTCCGGCGGCACCGCCGCCCGGATAACCCCCCTAGGCGGCCTAGACGAAAACCGCCACCAGCCCCTGTTGCACCACCCGGAAAAggg > 7:166078/1‑135 (MQ=255) gAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTTCCTGCGGCCCCGCGGCCGGTATCACCCCGCTAGGGGGCATCCAGTACAACGGCCAGCAGCCCCGGTTACACGCCCTGGCAAAggggg > 7:160513/1‑133 (MQ=255) cGATGTCGAGATCAGCATGCTGCACCGCTTCGTCGAAGCCGGTGCCTGGGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGccc < 3:291659/135‑1 (MQ=255) tCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGCCGTTATCACCCCGCTCGGCGGCATCCAGGACAACCGCCAGCGGCACGTGTTCCAAGCCCGGGAAAAGGTAGGCCCGGGTa > 3:122397/1‑135 (MQ=255) tCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTAAAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAc > 7:293760/1‑136 (MQ=255) cGATATAAGCAAGCGGGACCGCTTCGTCCAAGCGGGGGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTAcc < 8:49845/136‑1 (MQ=255) cGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGCCGGTTTAACCCCGATCCGCGGCATCGCGTACAACGGCAACCTGCACCGGTTCCCCGCCCGGGAAAAGGTCGGCCCGGTTTcc > 8:231789/1‑136 (MQ=255) ccgCAAGCTGCCCGGGTGCGGCGACGCGGGTGCGGGCGCCCCCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGaa < 1:19251/133‑1 (MQ=255) gCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGCCGTTTTCCCCCCGCTCGGCGGCCTCGAGTACAACAGCAAGCTGCCCGTGTTCCACGACCGGGAAAAGGTCGGCCCGGTTACCCAGAAGctcg > 3:261580/1‑133 (MQ=255) gACCGCTTCGTCGAAGCCGGTGCCTGCAGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGGTACCCAGAAGCTCTACaa > 3:202477/1‑134 (MQ=255) gCTTCGTCGAAGCCGGTGCCTGCGGCCCCGCGGCCGGTTTCACCCCGCTCGGCGGGCTCGAGGTCAACGGCCAGCTGCACGTGTTGCACCCCCCGGAAAAGGTCGGCCCGGGTACCCAGAACCTCGACAACGAGc > 2:29983/1‑135 (MQ=255) gCTTCGGCGACGCCGGTGCCGGGGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGc < 4:169245/135‑1 (MQ=255) gCTGCGCCGAAGCGGGGGCGGGCGGCCCCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGc < 3:67213/135‑1 (MQ=255) gcggcgACGCGGGGGCCGGCGCCCCCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGa < 3:15611/131‑1 (MQ=255) cACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACAGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGt < 4:261580/135‑1 (MQ=255) | TCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGTTCCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGT > ACB122/3977352‑3977614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |