Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 444,535 A→C 24.7% L130V (TTG→GTG)  hdfR_1 ← HTH‑type transcriptional regulator HdfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122444,5350AC24.7% 83.1 / 6.1 69L130V (TTG→GTG) hdfR_1HTH‑type transcriptional regulator HdfR
Reads supporting (aligned to +/- strand):  ref base A (36/14);  new base C (3/16);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 3.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGGTATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCTTG  >  ACB122/444403‑444668
                                                                                                                                    |                                                                                                                                     
ggtgggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAAccc                                                                                                                                    <  7:233866/136‑1 (MQ=255)
 gtgggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCGGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAAccc                                                                                                                                    <  6:260811/135‑1 (MQ=255)
   gggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGTCGGCAAATAGCTGATGCCGATGTCCAGGTTGACGTTGAGCAGGCGCCGTTCGATCTCCACCCCCGa                                                                                                                                 >  7:169102/1‑136 (MQ=255)
   gggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCg                                                                                                                                  <  1:262232/135‑1 (MQ=255)
     gtATCGGGTATGACCAGTTGCAGCTCATCTTCGTCCAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGAAGGCGCCGTGCGATCTCCAACCCCGa                                                                                                                                 >  5:245527/1‑134 (MQ=255)
                  ccAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCt                                                                                                                   >  2:113279/1‑135 (MQ=255)
                       tGCAGCTCATCTTCTAACAACACCAGGCCAGGCCGCCCTGGGGGGCGCGGCGGAAAATAGCTGATGCCGATGTCCCGGTTGCCGTGGAGCAGGCGCCGTTCGATCTCCAACCCCCAAAACTCGTAAATCGGCACg                                                                                                              <  6:102728/135‑1 (MQ=255)
                         cAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGa                                                                                                             >  2:6436/1‑134 (MQ=255)
                         cAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGa                                                                                                             >  1:22407/1‑134 (MQ=255)
                                   tagTACAACAGAAGGCCATGCAGCCCTGGCTGGCGAGGCGGCAAATAGCTGATGCCGAAGTCCAGGTTGCCGTAGAGCAGGCGCCGTTCGATCTCCAACCCCGACACCTCGTAAATCTGAACGACCAGGTGAGGtt                                                                                                 >  8:228249/3‑136 (MQ=255)
                                   tCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGtt                                                                                                 >  7:254364/1‑136 (MQ=255)
                                       acaacaGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCCACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAg                                                                                              >  1:359168/1‑135 (MQ=255)
                                       acaacaGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAg                                                                                              >  3:117353/1‑135 (MQ=255)
                                              cagGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTgc                                                                                        >  6:155258/1‑134 (MQ=255)
                                                 gCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACCACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGc                                                                                   >  7:228133/1‑136 (MQ=255)
                                                 gCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAg                                                                                    >  4:72721/1‑135 (MQ=255)
                                                 gCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGc                                                                                   >  7:373025/1‑136 (MQ=255)
                                                   cATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCg                                                                                 >  7:31620/1‑136 (MQ=255)
                                                    aTGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGGAAATCTGCCCGGCCAGGTGAGGTTGAGCCTTGCGCAGCCGt                                                                                >  7:358847/1‑136 (MQ=255)
                                                      gCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGt                                                                                >  4:162726/1‑134 (MQ=255)
                                                        cgCCCGGGCGGGCGCGGCGGCAAATAGCTGATGCCGATGTCCCGGTGGCCGGTGAGCCGGCGCCGTGCGATCACCACCCCCCACACCTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCa                                                                             <  2:110903/134‑1 (MQ=255)
                                                        aGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCACCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGtt                                                                               >  4:109623/1‑133 (MQ=255)
                                                        aGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGtt                                                                               >  1:112113/1‑133 (MQ=255)
                                                        aGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTc                                                                              >  7:264593/1‑134 (MQ=255)
                                                               gctggcGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGCTCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCt                                                                      >  3:11503/1‑135 (MQ=255)
                                                                  ggcGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCAGGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCg                                                                   >  1:50196/1‑135 (MQ=255)
                                                                  ggcGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGCCGCGGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGACCCTTGCGCAGCCGATCAAGCAGCTGCgg                                                                  >  7:17943/1‑136 (MQ=255)
                                                                   gggcggcgGCAAATCCCTGCTGCCGCGGTCCTGGTGGCCGTGGCGCCGGCGCCGGGCCAGCTCCACCCCCGCCAACGCGTAACGCGGCACGACCAGGGGCGGGTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                  <  4:372991/133‑1 (MQ=255)
                                                                   gcgcGGCGGCAATTCGCTGAGCCCTAGCGCCAGGGTGCCGTTCCGCCGGCGCCGTGCGAGCTCCACCCCCGACACCACGAAAAGCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                  <  4:366633/135‑1 (MQ=255)
                                                                   gcgcGGCGGCAATTAGCTGTGGCCGTGGCCCTGGGTGCCGGTGCGCCGGCGCCGTCCCACCCCCCCCCCCTACAACTGGAACATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                  <  2:138136/135‑1 (MQ=255)
                                                                       ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCCACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGAt                                                               >  7:301753/1‑134 (MQ=255)
                                                                       ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGa                                                                >  6:204292/1‑133 (MQ=255)
                                                                       ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGa                                                                >  1:160700/1‑133 (MQ=255)
                                                                       ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGAt                                                               >  8:245292/1‑134 (MQ=255)
                                                                       ggcggcAAATAGCTGATGCCGATGTCCAGGTTGACGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGAACGACCAGTTGAGGTTGAGCCTTGCGCAGCCGGTCAAGCAGCTGCTGGa                                                                >  2:119799/1‑133 (MQ=255)
                                                                         cggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAg                                                            >  3:4229/1‑135 (MQ=255)
                                                                            cAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGCCAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCtt                                                        >  8:256937/1‑136 (MQ=255)
                                                                                tcgctgctGCCGATGTCCAGGGTGCCGTGGAGCCGGCGCCGTTCCATCTCCAACCCCGCCAACTCGAAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGc                                                     <  2:85272/133‑1 (MQ=255)
                                                                                tcgCTGAGGCCGAGGTCCCGGTTGCCGTTGCGCCGGCGCCGTTCGAGCTCCACCCCCCACAACTCGTAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  8:31620/134‑1 (MQ=255)
                                                                                tAGCTGATGCCCTGGTCCTGGTGGCCGGTGATCCGGCGCGGATCTATCTCCAACCCCGACACCTCTTAAATATGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  7:2688/136‑1 (MQ=255)
                                                                                 cgCTGATGCCGTTGTCCAGGTTGCCGTTGAGCCGGCGCCGTTCGATCTCCACCCCCTACAACACGTAAAGCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  3:162726/134‑1 (MQ=255)
                                                                                 cccTGAGGCCGATGTCCCGGTGGCCGTGCCGCAGGCGCCGTTCGATCGCCACCCCCGACAACTCCTAACAGCGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  1:53465/133‑1 (MQ=255)
                                                                                 aGCTGATGCCGATGTCCCGGTTGCCGTTGCGCAGGCGCCGTTCGATCCCCACCCCCGACAACTCGTAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  1:6436/135‑1 (MQ=255)
                                                                                  gCTGATGCCGATGTCCCGGGTGCGGTTGAGCAGGCGCCGTGCGATCTCCACCCCCGACAACTCGAAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  2:112113/134‑1 (MQ=255)
                                                                                  gCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCCACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  4:117353/134‑1 (MQ=255)
                                                                                  gCTGAGGCCGATGTCCTGGTGGCCGTTGCGCAGGCGGCGTTCTAGCCCCACCCCCCACAACTCCTAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                    <  4:4229/134‑1 (MQ=255)
                                                                                     gctcccGATGTCCAGGTGGCCGTGCCGCTGGCGCGGTTCGACCTCCACCCCCCACAACTCTAAACTCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                                <  3:380227/131‑1 (MQ=255)
                                                                                     gatgccgatgccCAGGTGGCCGTTGAGCAGCCGCCGTTCGAGCCCCACCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                                <  3:378235/135‑1 (MQ=255)
                                                                                      aggCCGATGTCCAGGTGGCGTTTGCGCAGGCGCCGTTCTATCACCAACCCCCACAACTCGTAAAGCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                                <  8:370851/132‑1 (MQ=255)
                                                                                      aTGCCGATGTCAAGGTTGCCGTTGCGCAGGCGACGTTCGATCTCCCCCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCCGGCTTGGCCGCCCg                                               >  1:349280/1‑135 (MQ=255)
                                                                                      aTGCCGAGGGCCAGGGTGCCGTTGAGCAGGCGCCGTTCGAGCGCCACCCCCGACAACTCGTAAAGCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                                <  8:63840/134‑1 (MQ=255)
                                                                                        gCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTc                                            >  8:243431/1‑136 (MQ=255)
                                                                                         ccGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCCACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTTCGGGATCAGGATTGGCCGCACGGTc                                            >  5:71928/1‑135 (MQ=255)
                                                                                           gATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCt                                           >  1:76087/1‑134 (MQ=255)
                                                                                                ccAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAAt                                      >  6:224253/1‑134 (MQ=255)
                                                                                                cacgtttGACGTTGAGCTGGCGGCGTTCCATCTCCAACCGCGTATACTGGTAAATCAGCACGACCAGTTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATGAGGCTTGGCCGCTCTGTGTGCAATac                                    <  8:17943/131‑1 (MQ=255)
                                                                                                 cAGGTTGCGGTTGAGCTGGCGCCGTTCGAGCGCCACCCCCGACACCTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATac                                    <  6:189715/135‑1 (MQ=255)
                                                                                                     gtGCCGTGGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATacac                                  <  6:142621/132‑1 (MQ=255)
                                                                                                      tGCCGTTGAGATGGCGCCGTTCGATCTCCAACCCCGACAATTCTTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGa                               <  2:42907/135‑1 (MQ=255)
                                                                                                         ggTTGAGCCGGCGCGGTTCGATCACCCCCCCCGACAACTCTTAAATCCGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAg                            <  2:359168/134‑1 (MQ=255)
                                                                                                          gTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAg                            >  4:138203/1‑134 (MQ=255)
                                                                                                          gTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAg                            >  5:158047/1‑134 (MQ=255)
                                                                                                          gTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAg                            >  2:73667/1‑134 (MQ=255)
                                                                                                            tGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAAGCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCa                         <  3:134016/135‑1 (MQ=255)
                                                                                                                      ccGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCGCGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                  <  1:16955/84‑1 (MQ=255)
                                                                                                                      ccGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCGCGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                  >  2:16955/1‑84 (MQ=255)
                                                                                                                        gTTCGAACACCACCCCCCACACCTCTAAAAGCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGa            <  8:373025/136‑1 (MQ=255)
                                                                                                                                tcCAACCCCGACAACTCGTAAATCTTCACGACCAGGTGAGGTTGAGACTTGCGCAGCCGTTCAAGCATCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCt    >  8:170976/1‑136 (MQ=255)
                                                                                                                                  cAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCTTg  <  7:245292/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GGTGGGTATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCTTG  >  ACB122/444403‑444668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑