Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,403,899 A→T 5.1% E108V (GAA→GTA)  CJ019_04165 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,403,8990AT 5.1% 235.1 / 2.5 78E108V (GAA→GTA) CJ019_04165hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (48/26);  new base T (2/2);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

TGGCGCCAGCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGATCGATG  >  ACB122/4403767‑4404034
                                                                                                                                    |                                                                                                                                       
tGGCGCCAGCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGaagaag                                                                                                                                       >  3:180690/1‑135 (MQ=255)
tGGCGCCAGCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAgg                                                                                                                                      >  7:175215/1‑136 (MQ=255)
   cgcCAGCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCCCCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCg                                                                                                                                    >  4:165022/1‑135 (MQ=255)
    gcCACCAATGCGCAGCGGCGGGAGCGCGGGGGGCTCGCCTAAGCACCGATGGGTGCCAGGCGAGTACGTTATGGCCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGt                                                                                                                                   <  6:139127/135‑1 (MQ=255)
        gCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGTAGGCGTCAAc                                                                                                                               >  3:364686/1‑135 (MQ=255)
        gCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACAGATTGGTGCCAGGCGAGTACGGTATGGTCACGCCCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGTAGGCGTCCAc                                                                                                                               >  3:371016/1‑135 (MQ=255)
         cAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGACCTGCTTTTCGAGAACGAAGAAGGCGTCAAcc                                                                                                                              >  1:350401/1‑135 (MQ=255)
              cgccggggCTGGATCGCGGTGTGCGAGCGGATTCACCTATTGGTGCCAGGGGAGTACGTTATGTGCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGc                                                                                                                          <  2:225185/128‑1 (MQ=255)
                    ggCGGGAGCGCGGTGTGCGAGCCTAATCACGGTGGGGGGCCCGGCGAGTAGGTTTTGGCCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTc                                                                                                                   <  5:115782/135‑1 (MQ=255)
                             gCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGACTCGGTCAACGCAGc                                                                                                           >  2:59345/1‑134 (MQ=255)
                                gtgtGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTAc                                                                                                       >  3:102823/1‑135 (MQ=255)
                                  gtgCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGCTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACgg                                                                                                     >  4:89129/1‑135 (MQ=255)
                                      gAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGaa                                                                                                 >  2:38488/1‑135 (MQ=255)
                                       aGCCTAATCACCGATTGGGGCCCGGCGAGTACGTTATGGCCACGACCGTAGACGGCCAAAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAAc                                                                                                <  3:262475/135‑1 (MQ=255)
                                       aGCCTAATCACAGATTGGGGCCAGGCTCGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAAc                                                                                                <  4:203341/135‑1 (MQ=255)
                                                aCCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGa                                                                                       >  4:305586/1‑135 (MQ=255)
                                                     ttGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGca                                                                                  >  1:365308/1‑134 (MQ=255)
                                                              ggCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGAc                                                                         >  2:169240/1‑135 (MQ=255)
                                                                 gAGTAGGTTAGGGACACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCg                                                                     <  8:180975/136‑1 (MQ=255)
                                                                      cGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCgaga                                                                  >  5:178010/1‑134 (MQ=255)
                                                                       gTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGaaaa                                                               >  7:19766/1‑136 (MQ=255)
                                                                            ggTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCtt                                                                                                                            <  3:127087/70‑1 (MQ=255)
                                                                            ggTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCtt                                                                                                                            >  4:127087/1‑70 (MQ=255)
                                                                            ggTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCa                                                           >  2:211568/1‑135 (MQ=255)
                                                                            ggTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCa                                                           >  3:338800/1‑135 (MQ=255)
                                                                            ggTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCa                                                           >  1:319333/1‑135 (MQ=255)
                                                                            ggTCACGACAGTAGACGGCCAGAGCATGGTCTAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTc                                                            >  6:118761/1‑134 (MQ=255)
                                                                              tCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCAc                                                          <  1:211568/134‑1 (MQ=255)
                                                                              tCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTa                                                        <  7:315358/136‑1 (MQ=255)
                                                                              tCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTa                                                        <  7:21582/136‑1 (MQ=255)
                                                                               cccGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTa                                                        <  1:104821/133‑1 (MQ=255)
                                                                               cccGACCGTAGACGGCCAGAGCATGGGCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTa                                                        <  6:383266/133‑1 (MQ=255)
                                                                               cACGACCGTAGACTGCCAGAGCCTGGTCAAGGAAAGTCTTTTAGAGAACGAAGACGGCGTCAACCTTGCCTCGTTCAACGCAGCTGACGGCGAACGCCGTGTGATCGCCTGGGCTGACATCGACAAAATTCACTa                                                        <  6:267355/135‑1 (MQ=255)
                                                                                   aCCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTc                                                    >  6:74159/1‑135 (MQ=255)
                                                                                    ccGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCgg                                                  <  8:267315/136‑1 (MQ=255)
                                                                                    ccGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCg                                                   <  1:38488/135‑1 (MQ=255)
                                                                                    ccGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCg                                                   >  6:226694/1‑135 (MQ=255)
                                                                                     cgtttaCGGCCAGAGCATGTTCAAGGAACTGCTTTTCGATATCGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCTGACATCTAGAAAATTCTCTACTTCgg                                                  <  6:296077/130‑1 (MQ=255)
                                                                                     cGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCgg                                                  <  6:109680/135‑1 (MQ=255)
                                                                                     cGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCgg                                                  <  1:376183/135‑1 (MQ=255)
                                                                                     cGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCg                                                   >  1:109937/1‑134 (MQ=255)
                                                                                          atggCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAata                                             >  5:50084/3‑135 (MQ=255)
                                                                                          aCGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAata                                             >  6:129767/1‑135 (MQ=255)
                                                                                          aCGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAata                                             >  3:334765/1‑135 (MQ=255)
                                                                                          aCGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAata                                             >  1:350768/1‑135 (MQ=255)
                                                                                          aCGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACAGCGAGAAAATTCACTACGTCGGCAata                                             >  6:5347/1‑135 (MQ=255)
                                                                                                 gagCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACTCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGc                                      >  6:85272/1‑135 (MQ=255)
                                                                                                 gagCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGc                                      >  4:188560/1‑135 (MQ=255)
                                                                                                 gagCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCt                                     >  8:291041/1‑136 (MQ=255)
                                                                                                 gagCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCt                                     >  7:22338/1‑136 (MQ=255)
                                                                                                 gagCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCATGGGCGGACATCGAGAAAATTCACAACGTCGGCAAAATTCTGGCt                                     >  7:142950/1‑136 (MQ=255)
                                                                                                     aTGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCcaa                                 <  7:68394/136‑1 (MQ=255)
                                                                                                     aTGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCcaa                                 <  8:225184/136‑1 (MQ=255)
                                                                                                     aTGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCca                                  >  2:338542/1‑135 (MQ=255)
                                                                                                      tGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTTGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCcaa                                 <  2:350768/135‑1 (MQ=255)
                                                                                                      tGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCcaa                                 <  3:57425/135‑1 (MQ=255)
                                                                                                          cAAGGAACTGATTTTCGAGAACGAAGAAGGCGTCAACCTTCCCTCGGACAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGCCGGACATCGAGAAAATTCACTACCTCGGCAATATTCTGGCTCcaagcaa                             >  5:241296/1‑135 (MQ=255)
                                                                                                           aaGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCTGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAgg                           >  7:75717/1‑136 (MQ=255)
                                                                                                           aaGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCcaagcaag                            >  5:203785/1‑135 (MQ=255)
                                                                                                           aaGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGCTCAACGCATATTACTGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTGACTACGGCGGCATTATTCTGGCTCcgcgcaag                            >  5:33213/1‑135 (MQ=255)
                                                                                                               aaCTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACTTCGGCAATATTCTGGCTCCAAGCAAGGTTc                        >  1:213524/1‑135 (MQ=255)
                                                                                                               aaCTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGATTc                        >  3:197627/1‑135 (MQ=255)
                                                                                                               aaCTGCTTTTCGAGAACGAAGAAGGCGTAAAACTTGACTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGCTTc                        >  1:70189/1‑135 (MQ=255)
                                                                                                                  tGCTTTTCGAGAACGAAGTAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTg                     <  4:364686/135‑1 (MQ=255)
                                                                                                                  tGCTTTTCGAGAACGAAGTAGGCGTCAACCTTGCCTCGGTCAACGCAGATTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTg                     <  4:371016/135‑1 (MQ=255)
                                                                                                                  tGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTAAGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTg                     <  5:118761/135‑1 (MQ=255)
                                                                                                                  tGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGGCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTg                     <  2:350401/135‑1 (MQ=255)
                                                                                                                    cTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGACCGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGcc                  >  7:228241/1‑136 (MQ=255)
                                                                                                                    cTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAATATTCACTACGTCGGCAATATTCTGGCTCCATGCAAGGTTCTTGGcc                  >  7:228239/1‑136 (MQ=255)
                                                                                                                    cTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGc                   >  5:150829/1‑135 (MQ=255)
                                                                                                                     ttttCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGcc                  >  5:160926/1‑135 (MQ=255)
                                                                                                                     ttttCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCg                 >  8:306818/1‑136 (MQ=255)
                                                                                                                     ttttCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCg                 >  7:177551/1‑136 (MQ=255)
                                                                                                                      tttCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGt                          <  4:355780/126‑1 (MQ=255)
                                                                                                                      tttCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGt                          >  3:355780/1‑126 (MQ=255)
                                                                                                                          gagaACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAatat                                            <  2:78980/104‑1 (MQ=255)
                                                                                                                          gagaACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAatat                                            >  1:78980/1‑104 (MQ=255)
                                                                                                                          gagaACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGttt              >  2:105125/1‑134 (MQ=255)
                                                                                                                               cGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGa        >  1:240877/1‑135 (MQ=255)
                                                                                                                                gaagaaGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGAt       <  3:305586/135‑1 (MQ=255)
                                                                                                                                 aagaagGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGAt       >  6:111205/1‑134 (MQ=255)
                                                                                                                                 aagaagGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGAt       >  4:128450/1‑134 (MQ=255)
                                                                                                                                 aagaagGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGATc      <  5:268511/135‑1 (MQ=255)
                                                                                                                                 aagaagGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGATTCTTGGCCGTTTCTAGGATc      <  4:197627/135‑1 (MQ=255)
                                                                                                                                    aagGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGATCGATg  <  7:202238/136‑1 (MQ=255)
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TGGCGCCAGCAATTCGCAGCGGCTGGATCGCCGTGTGCGAGCCGAATCACCGATTGGTGCCAGGCGAGTACGTTATGGTCACGACCGTAGACGGCCAGAGCATGGTCAAGGAACTGCTTTTCGAGAACGAAGAAGGCGTCAACCTTGCCTCGGTCAACGCAGCTTACGGCGAACGCCGAGTGATCGCCTGGGCGGACATCGAGAAAATTCACTACGTCGGCAATATTCTGGCTCCAAGCAAGGTTCTTGGCCGTTTCTAGGATCGATG  >  ACB122/4403767‑4404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑