Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,711,854 G→A 8.2% L109L (CTG→CTA)  CJ019_04448 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,711,8540GA 8.2% 154.1 / 4.3 49L109L (CTG→CTA) CJ019_04448hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/28);  new base A (2/2);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CTACCTGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGCCTGGGTGTGCGTATCT  >  ACB122/4711722‑4711987
                                                                                                                                    |                                                                                                                                     
ctacctGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGc                                                                                                                                     <  1:173684/135‑1 (MQ=255)
    ctGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATg                                                                                                                                <  8:10929/136‑1 (MQ=255)
     tGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATg                                                                                                                                <  2:231195/135‑1 (MQ=255)
     tGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGAATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATg                                                                                                                                <  6:144052/135‑1 (MQ=255)
        tCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGt                                                                                                                              <  1:126887/134‑1 (MQ=255)
        tCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGt                                                                                                                              <  2:145941/134‑1 (MQ=255)
        tCGAAGCTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGt                                                                                                                              <  5:312922/134‑1 (MQ=255)
         cGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTc                                                                                                                            <  6:296126/135‑1 (MQ=255)
               ggCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGGGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCTGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCt                                                                                                                      <  2:200696/134‑1 (MQ=255)
                 cAATCCTGCCCAAGGCCAGCCTCGGCGCGGCGCCGCGGGGGCGGCCCGGTGAGGGCAGCGGGAGGACCGCAGGCAGCGGCAGCGCCCAGGTCGAGCGCACCCCGGACGAGGCCCTGGCCCATGGTGCACGCCTGc                                                                                                                    <  6:62128/135‑1 (MQ=255)
                   aTCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGGGCTGCCCGGTGATGTCAGCTGGACGACCGGAGGCAGCGGCATCGCCCATGACGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATg                                                                                                                 <  8:124364/136‑1 (MQ=255)
                     ccTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATgg                                                                                                                <  5:160756/135‑1 (MQ=255)
                      cTGCACAAGGACAGCCTGGGCTCGGCGCAGCGGGTGCTGCCCGGGGTTGTGAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGACGATGCCCTGGCCCATGGTTCACGCCAGCATggg                                                                                                               <  2:316054/135‑1 (MQ=255)
                           caAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCAGCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCa                                                                                                          <  6:382204/135‑1 (MQ=255)
                            aaGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAg                                                                                                         >  2:34311/1‑135 (MQ=255)
                            aaGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTCCATGGGCTGCAg                                                                                                         >  5:301155/1‑135 (MQ=255)
                                      tcggctcggcGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTAtggctg                                                                                                >  3:276887/1‑134 (MQ=255)
                                           tcggcGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCg                                                                                          >  5:167421/1‑135 (MQ=255)
                                                  aGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCCCGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCAcg                                                                                     >  1:21027/1‑133 (MQ=255)
                                                     gggTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCCCCCCGGAAGATGCCCTGGCCCATGGTTCCCGCCTGCATGGGCTGCCGGTATGGCTGGCATCGCCCCGCGAGt                                                                                >  4:54900/1‑135 (MQ=255)
                                                            gcCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATgg                                                                                                                               >  2:295883/1‑81 (MQ=255)
                                                            gcCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATgg                                                                                                                               <  1:295883/81‑1 (MQ=255)
                                                                 gTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGccc                                                                   <  7:258807/136‑1 (MQ=255)
                                                                 gTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGcc                                                                    <  5:111057/135‑1 (MQ=255)
                                                                 gTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGcc                                                                    <  4:26992/135‑1 (MQ=255)
                                                                   gATGTCAGCTGGATGTCCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCg                                                                  <  3:370600/135‑1 (MQ=255)
                                                                   gATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCg                                                                  <  1:249228/135‑1 (MQ=255)
                                                                    aTGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCg                                                                  >  4:349087/1‑134 (MQ=255)
                                                                       tCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAg                                                             <  7:39258/136‑1 (MQ=255)
                                                                        cAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGTGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAg                                                             >  6:149764/1‑135 (MQ=255)
                                                                                   ccGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATc                                                  >  4:370483/1‑135 (MQ=255)
                                                                                           agCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCccgccg                                         >  8:99294/1‑136 (MQ=255)
                                                                                                cATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAg                                     >  4:20591/1‑135 (MQ=255)
                                                                                                cATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTAGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAg                                     >  3:346849/1‑135 (MQ=255)
                                                                                                cATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTAGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAg                                     >  3:352668/1‑135 (MQ=255)
                                                                                                     cccATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGcc                               <  7:76279/136‑1 (MQ=255)
                                                                                                            cGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAg                         <  6:167421/135‑1 (MQ=255)
                                                                                                                 gcACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACa                    >  6:172904/1‑135 (MQ=255)
                                                                                                                    ccccGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGcc                 <  3:349087/135‑1 (MQ=255)
                                                                                                                    ccccGGAAGATGCCCTAGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGcc                 <  4:346849/135‑1 (MQ=255)
                                                                                                                    ccccGGAAGATGCCCTAGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGcc                 <  4:352668/135‑1 (MQ=255)
                                                                                                                        ggAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTGAGGGACCGCCgggg             >  1:337916/1‑135 (MQ=255)
                                                                                                                         gAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGCCTGGgt            <  1:34311/135‑1 (MQ=255)
                                                                                                                             aTGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGCCTGGGTGTGc        <  3:161107/135‑1 (MQ=255)
                                                                                                                                  cTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTTAGCAAGGCCCg                                                                  <  3:281874/72‑1 (MQ=255)
                                                                                                                                  cTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCg                                                                  >  4:281874/1‑72 (MQ=255)
                                                                                                                                  cTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAg                         >  2:73619/1‑113 (MQ=255)
                                                                                                                                  cTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGCCTGGGTGTGCGTATCt  >  7:235664/1‑136 (MQ=255)
                                                                                                                                  cTGGCACATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAg                         <  1:73619/113‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CTACCTGTTCGAAGGTGCAATCCTGCACAAGGACAGCCTCGGCTCGGCGCAGCGGGTGCTGCCCGGTGATGTCAGCTGGATGACCGCAGGCAGCGGCATCGCCCATGTCGAGCGCACCCCGGAAGATGCCCTGGCCCATGGTTCACGCCTGCATGGGCTGCAGGTATGGCTGGCATCGCCACGCGAGTTTGAGCAAGGCCCGGCAAGCTACAGCCATCACCCCGCCGCGAGCTTGCCCGTCAGTGACAGCCTGGGTGTGCGTATCT  >  ACB122/4711722‑4711987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑