| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,742,598 | G→A | 6.4% | P397L (CCG→CTG) | proP_5 ← | Proline/betaine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,742,598 | 0 | G | A | 6.4% | 199.2 / 3.3 | 62 | P397L (CCG→CTG) | proP_5 | Proline/betaine transporter |
| Reads supporting (aligned to +/- strand): ref base G (24/34); new base A (2/2); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
TTTGTCGCCGCACATGACCGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCACCACCCCCACC > ACB122/4742465‑4742732 | tttGTCGCCGCACATGACCGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATTATCAGACAAAACCCGACACACACCCATGGGCTCCACgg < 1:72522/135‑1 (MQ=255)tttGTCGCCGCACATGACCGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACg < 6:310189/134‑1 (MQ=255) cgccgcACATGACCGGTATCGGCTCCGCCGCAACCACCAATGGCGCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCa < 2:181361/135‑1 (MQ=255) cgcACATGACCGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAgc > 4:377635/1‑134 (MQ=255) ccGGCATCGGCCCCGCCCAAAACCCCAAGGCCCCACGACGCCCGAAATACCGGGCCGGCAGCAGCCCCA‑CCGCGCCCATGATCACACAAACCCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGc < 7:295025/136‑1 (MQ=255) cGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGc < 3:316930/135‑1 (MQ=255) cGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGc < 4:330356/135‑1 (MQ=255) tCGGCTCCGCCGCAAACCCCAATGGCTCACGCCGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 7:120962/135‑1 (MQ=255) tCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 8:31734/135‑1 (MQ=255) tCGGCTCCGCCGCAAACACCAAGGCCACAGGACGCCCGAACTCCCGGCCCGGCAGCAGCCCCA‑CCGCCCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 7:272959/135‑1 (MQ=255) cGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 6:157428/134‑1 (MQ=255) cGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGGCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 4:192821/134‑1 (MQ=255) cGGCGCCGCCTCAAACACCACTGGCTCACGACGCCCGAAATCCCTGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAACACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 2:95937/134‑1 (MQ=255) cTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTg < 7:336868/136‑1 (MQ=255) tCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTg < 2:284757/135‑1 (MQ=255) tCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTg < 6:152367/135‑1 (MQ=255) tCCGCCGAAAACACCAATGGCTCACGACGACCTAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACAGCATCAGCGCAATCAGTGCCAACGGCGTg < 2:64234/135‑1 (MQ=255) ccgccgCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTgg > 8:124800/1‑135 (MQ=255) ccgcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGCCACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTAc > 3:384745/1‑135 (MQ=255) ccgcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCCCGGCATCAGCGCAATCAGTGCCCACGGCGTGGTAc > 1:207112/1‑135 (MQ=255) cgcAAACCCCAATGGCTCACGACGCCCGAAATACCGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACt < 1:129259/135‑1 (MQ=255) gcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAACCCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTg > 3:324355/1‑135 (MQ=255) gcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCCCGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTg > 5:128923/1‑135 (MQ=255) gcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTgg > 8:48172/1‑136 (MQ=255) gcAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACAGCATCAGCCCAATCAGTGCCAACGGCGTGGTACTg > 1:64234/1‑135 (MQ=255) aaaCACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGc < 3:377635/135‑1 (MQ=255) aaCACAAATGGCCACCGACCCCCGAAATACCGGGACGACAGCAGCCCAA‑CCGCGCCCATCATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGcc < 2:91473/135‑1 (MQ=255) acacCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAACCCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGccc < 2:45230/135‑1 (MQ=255) aaTGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTg < 7:231147/136‑1 (MQ=255) tGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCCTGATCAGACAAAACCCCACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGc > 6:126630/1‑135 (MQ=255) tGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCg > 8:145778/1‑136 (MQ=255) cacACGTCGCCCGAACTACCGGGCCGGCTGCAGCCCCA‑CCGCGCCCATGACCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTa < 6:27570/133‑1 (MQ=255) cTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATCATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTa < 5:105694/135‑1 (MQ=255) cACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACAACCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAgg < 6:305867/135‑1 (MQ=255) gacgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCCCGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCa > 3:53843/1‑135 (MQ=255) gacgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCa > 8:331633/1‑92 (MQ=255) gacgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCa < 7:331633/92‑1 (MQ=255) gacgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGccc > 3:326647/1‑134 (MQ=255) gacgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCAc < 7:71182/136‑1 (MQ=255) cgCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGt > 8:100509/1‑136 (MQ=255) cccccGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATTATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACg < 1:173768/133‑1 (MQ=255) gAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTa > 2:39332/1‑135 (MQ=255) aGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAgg < 7:145778/136‑1 (MQ=255) cagcagCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGcc > 3:128894/1‑135 (MQ=255) gcagcCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCAc > 3:194184/1‑112 (MQ=255) gcagcCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCAc < 4:194184/112‑1 (MQ=255) gcagcCCCA‑CCGCGCCCATGATAAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGATGTAGGTGAAGGAAATg > 4:111831/1‑131 (MQ=255) catccccA‑CCGCGCCCATGATCAGACAAACCCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGAt < 8:341774/133‑1 (MQ=255) agcCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCCTGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGAt < 3:76907/135‑1 (MQ=255) agcCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGAt < 1:9819/135‑1 (MQ=255) tccccACCCGCGCCCTTTACCACACAAACCCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGAt < 1:388828/134‑1 (MQ=255) gcCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTg < 2:115769/135‑1 (MQ=255) aCAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAg < 5:194846/135‑1 (MQ=255) aaaCCCGACACACACCCATGGGCTCCACAGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTg > 3:324748/1‑74 (MQ=255) aaaCCCGACACACACCCATGGGCTCCACAGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTg < 4:324748/74‑1 (MQ=255) ccGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg > 4:136042/1‑49 (MQ=255) ccGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACg < 3:136042/49‑1 (MQ=255) ccGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCcccccc > 1:322354/1‑135 (MQ=255) cacacCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCAcc > 8:33201/1‑135 (MQ=255) cacacCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCg > 7:66249/1‑136 (MQ=255) cATGGTCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTCCGTAGGCCACGTGGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGca > 7:286075/1‑136 (MQ=255) cATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGTTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGca > 8:397934/1‑136 (MQ=255) gCTCCACGGCATCAGCGCAATCAGTGACAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGcaccac < 4:324355/135‑1 (MQ=255) aCGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCACCCCCCcca > 3:243800/1‑135 (MQ=255) ggCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCCCCAcccccccc > 5:55550/1‑135 (MQ=255) ggCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCACCAcccccccc > 6:367551/1‑135 (MQ=255) ggCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCACCACCCccacc > 6:396500/1‑135 (MQ=255) | TTTGTCGCCGCACATGACCGGTATCGGCTCCGCCGCAAACACCAATGGCTCACGACGCCCGAAATACAGGGCCGTCAGCAGCCCCA‑CCGCGCCCATGATCAGACAAAACCCGACACACACCCATGGGCTCCACGGCATCAGCGCAATCAGTGCCAACGGCGTGGTACTGGCCCACAGTGCGTAGGCCACGTTGTAGGTGAAGGAAATGCCCGATACCCGGATCTGCGCCGGGAACAGCCCCACCATCACCGAGGGCACCACCCCCACC > ACB122/4742465‑4742732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |