Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 464,193 A→G 7.5% L598L (TTG→CTG)  rpoD ← RNA polymerase sigma factor RpoD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122464,1930AG 7.5% 164.1 / 4.1 53L598L (TTG→CTG) rpoDRNA polymerase sigma factor RpoD
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

GTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCACGTGCGGTCAGG  >  ACB122/464063‑464326
                                                                                                                                  |                                                                                                                                     
gtgCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGCAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACgac                                                                                                                                   >  3:97461/1‑133 (MQ=255)
    aaCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcg                                                                                                                               >  2:130737/1‑135 (MQ=255)
    aaCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACCCcttcg                                                                                                                               >  2:70377/1‑135 (MQ=255)
       ggAAGGCGGGCTTTATGTCACGGGCAACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgctt                                                                                                                            <  2:115994/135‑1 (MQ=255)
            gCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATc                                                                                                                       >  5:358130/1‑135 (MQ=255)
              gggCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTg                                                                                                                     >  6:210069/1‑135 (MQ=255)
                 cgtaaTGGCACGGGCCACTAAAGAAACACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTTCGCTACGCCTTCGCTTCGATCTGACg                                                                                                                  <  3:120966/131‑1 (MQ=255)
                                 aCTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCCGGTGCTCGCTGCGAGTCGGGTGGCGCCGCTTGCGCAACGCCGTCGCGTCGATCTGTCGGATCCGCTCAcgcggt                                                                                                  >  4:225288/1‑133 (MQ=255)
                                   tAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAGCGCCTTCGCTTCGATCTGACGGATCCGc                                                                                                           >  4:111459/1‑124 (MQ=255)
                                   tAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAGCGCCTTCGCTTCGATCTGACGGATCCGc                                                                                                           <  3:111459/124‑1 (MQ=255)
                                          acaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCGTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAAc                                                                                         <  1:283062/133‑1 (MQ=255)
                                           aaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCCCGCGGTACGTCAAACt                                                                                        >  3:62955/1‑135 (MQ=255)
                                           aaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCGTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAACCTg                                                                                       >  8:34844/1‑136 (MQ=255)
                                                cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTg                                                                                   <  5:393481/135‑1 (MQ=255)
                                                  gCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGcc                                                                                 <  1:54071/135‑1 (MQ=255)
                                                           ggggTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc                                                                        <  6:358130/135‑1 (MQ=255)
                                                           ggggTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc                                                                        <  5:196784/135‑1 (MQ=255)
                                                               ttttGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACCCCTTCGCTTCGATCTGCCGGATCCGCTCACGCGTTTCGTCAAACTGCATGCCCACCACTTGGAgg                                                                    >  2:362678/1‑135 (MQ=255)
                                                                    tcatcaGGCGTCGAGGACGGCGCGCAGGTGCTCGCTGCGAGGCGGGGGGCGCTGGTTGCGAAAGGCGTGCGCTTGGATCTGACGCATCCGCGCAGGCTTTACGTCAAACTGCTTGCCCCCCTCTCCGAGGGTGTgg                                                              <  8:117134/136‑1 (MQ=255)
                                                                    tcatcaCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGgtgt                                                                <  1:130737/134‑1 (MQ=255)
                                                                    tcatcaCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTgg                                                              <  8:102930/136‑1 (MQ=255)
                                                                    tcatcaCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGCGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGgtgt                                                                <  1:396467/134‑1 (MQ=255)
                                                                     catcaCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGtgtg                                                               <  3:388188/134‑1 (MQ=255)
                                                                        caCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCg                                                           <  5:210069/135‑1 (MQ=255)
                                                                          cTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGgt                                                         >  4:123222/1‑135 (MQ=255)
                                                                          cTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGGTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGgtg                                                        >  7:364436/1‑136 (MQ=255)
                                                                                gAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCAt                                                   >  1:141072/1‑135 (MQ=255)
                                                                                 aggaaggaGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCAt                                                   <  2:141072/134‑1 (MQ=255)
                                                                                 aggaaggaGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATg                                                  <  4:107993/135‑1 (MQ=255)
                                                                                 aggaaggaGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGt                                                 >  8:65295/1‑136 (MQ=255)
                                                                                        aGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGt                                                 <  5:182739/129‑1 (MQ=255)
                                                                                        aGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATAc                                           <  1:284282/135‑1 (MQ=255)
                                                                                        aGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATAc                                           <  2:40197/135‑1 (MQ=255)
                                                                                        aGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACCCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGt                                                 >  6:182739/1‑129 (MQ=255)
                                                                                          cgcAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGGTCATGTCGATAcc                                          >  3:52849/1‑134 (MQ=255)
                                                                                            cAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGaaa                                      <  7:45899/136‑1 (MQ=255)
                                                                                            cAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGGTCATGTCGATACCGaaa                                      >  7:30407/1‑136 (MQ=255)
                                                                                               gTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAAc                                     >  2:265905/1‑134 (MQ=255)
                                                                                               gTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAAc                                     >  2:66156/1‑134 (MQ=255)
                                                                                                  cTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCAt                                 <  4:280378/135‑1 (MQ=255)
                                                                                                     gCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATgcgc                             >  7:378075/1‑136 (MQ=255)
                                                                                                     gCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCGTCGCTTCGATCTGACGGATCCGCTCACGCGGTACGGCAAACTGCTTGCCCACCTCTTCGAGGGGGTGGTCGGTGGTCATGTCGCTTCCGAAACGccagcg                              >  5:203417/1‑130 (MQ=255)
                                                                                                       tGCGAGTCGGGTTGCGCAGCTTGCGCAACGCCTGCGCGTCGATCTGACGGTTCCGCTCACGCGTGACGGCAAACTGCTTGCCCACCGCCTCGAGGGTGTGGTCGGGGGTCATGTGGCTTCCGAAACGCATGCgca                            >  1:250791/1‑135 (MQ=255)
                                                                                                       tGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCCTGCgcag                           >  7:132120/1‑136 (MQ=255)
                                                                                                          gAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATACGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCgcagca                         >  3:26979/1‑135 (MQ=255)
                                                                                                           aGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAACCGCATGCGCAGCAc                        >  3:312628/1‑135 (MQ=255)
                                                                                                               gggTGACGCAGTTTGCGCAGCGCCTTCGCTTCGATCTGACGGATACGTTCGCGGGTAACGTCGAAc                                                                                         <  3:98293/66‑1 (MQ=255)
                                                                                                               gggTGACGCAGTTTGCGCAGCGCCTTCGCTTCGATCTGACGGATACGTTCGCGGGTAACGTCGAAc                                                                                         >  4:98293/1‑66 (MQ=255)
                                                                                                                    gcgcAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGCAACGCATGCGCCGCCCCTTGGCTTc               >  3:136061/1‑135 (MQ=255)
                                                                                                                     cgcAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCa              <  5:228712/135‑1 (MQ=255)
                                                                                                                     cgcAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGATCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCa              <  3:2457/135‑1 (MQ=255)
                                                                                                                           ttGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCACGTGCg        <  1:70377/135‑1 (MQ=255)
                                                                                                                              cgcAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCACGTGCgg       <  6:361592/133‑1 (MQ=255)
                                                                                                                                 aaCGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCACGTGCGGTCAgg  >  5:380732/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                     
GTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGGTCGGTGTTCATGTCGATACCGAAACGCATGCGCAGCACCTTGGCTTCACGTGCGGTCAGG  >  ACB122/464063‑464326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑