Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,009,692 G→C 7.3% A5G (GCT→GGT)  CJ019_04705 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,009,6920GC 7.3% 193.3 / 5.8 55A5G (GCT→GGT) CJ019_04705hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base C (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGAT  >  ACB122/5009566‑5009826
                                                                                                                              |                                                                                                                                      
ttGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGTAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTc                                                                                                                                 <  3:346911/134‑1 (MQ=255)
ttGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTc                                                                                                                                 <  6:126221/134‑1 (MQ=255)
      tCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATc                                                                                                                          <  6:306607/135‑1 (MQ=255)
           tGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCCCGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGGCATCCTAgc                                                                                                                     >  6:79489/1‑135 (MQ=255)
           tGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAgc                                                                                                                     >  6:79491/1‑135 (MQ=255)
            gggTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAgcg                                                                                                                    <  7:388959/135‑1 (MQ=255)
              gTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAgcgc                                                                                                                   <  1:37775/134‑1 (MQ=255)
                    cTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGtt                                                                                                            <  1:82539/135‑1 (MQ=255)
                     tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGc                                                                                                                                       <  1:56642/107‑1 (MQ=255)
                     tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGc                                                                                                                                       >  2:56642/1‑107 (MQ=255)
                     tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTc                                                                                                                                 >  6:125378/1‑113 (MQ=255)
                     tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTc                                                                                                                                 <  5:125378/113‑1 (MQ=255)
                     tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGttt                                                                                                           >  7:36432/1‑135 (MQ=255)
                        cccGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGa                                                                                                         <  1:311068/134‑1 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCATGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGACCCAGCTc                                                                                                                                 <  5:81848/103‑1 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCATGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGACCCAGCTc                                                                                                                                 <  5:76075/103‑1 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCAGGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGACCCAGCTc                                                                                                                                 >  6:76075/1‑103 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCAGGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGACCCAGCTc                                                                                                                                 >  6:81848/1‑103 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCTGTCATCCTAGCGCTCTCGTTTGAGTCCACga                                                                                                 >  4:382531/1‑135 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACga                                                                                                 >  3:202033/1‑135 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACga                                                                                                 >  2:163570/1‑135 (MQ=255)
                               ggATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTGGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgct                                                                                                >  7:85536/1‑134 (MQ=255)
                                          cGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGaa                                                                                        >  2:74388/1‑133 (MQ=255)
                                            tAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTGTGAGTCCACGATGAGATGAATAAGc                                                                                   >  7:384602/1‑136 (MQ=255)
                                               ccGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGccc                                                                                 <  4:359168/135‑1 (MQ=255)
                                                      cAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCgg                                                                          >  3:216110/1‑135 (MQ=255)
                                                      cAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCgg                                                                          >  6:397234/1‑135 (MQ=255)
                                                      cAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCgg                                                                          >  5:190121/1‑135 (MQ=255)
                                                            gACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCg                                                                    <  4:244644/135‑1 (MQ=255)
                                                            gACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCg                                                                    <  3:61652/135‑1 (MQ=255)
                                                                   gggCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGc                                                            <  8:36432/136‑1 (MQ=255)
                                                                          aaGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTAc                                                      <  6:190121/135‑1 (MQ=255)
                                                                            gCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACtt                                                    <  3:25297/135‑1 (MQ=255)
                                                                                   ggCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGcc                                            >  7:247219/1‑136 (MQ=255)
                                                                                    gCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGGCTCCGGCGCGGGCTTTTACTTTCCgg                                               <  1:74388/132‑1 (MQ=255)
                                                                                     cAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCtt                                          <  7:258282/136‑1 (MQ=255)
                                                                                     cAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGGCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCtt                                          <  8:384602/136‑1 (MQ=255)
                                                                                      aGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCtt                                          <  3:242826/135‑1 (MQ=255)
                                                                                           tGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCa                                                                              >  1:295534/1‑94 (MQ=255)
                                                                                           tGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCa                                                                              <  2:295534/94‑1 (MQ=255)
                                                                                                gTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATc                                >  2:223299/1‑135 (MQ=255)
                                                                                                 tataGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCt                               <  6:78249/135‑1 (MQ=255)
                                                                                                     ggTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAcc                           >  4:287298/1‑135 (MQ=255)
                                                                                                     ggTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATATGAccc                          >  7:12763/1‑136 (MQ=255)
                                                                                                      gTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAcc                           <  5:106522/134‑1 (MQ=255)
                                                                                                      gTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAcc                           >  4:16904/1‑134 (MQ=255)
                                                                                                           cACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCg                                                                    <  1:277882/88‑1 (MQ=255)
                                                                                                           cACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCg                                                                    >  2:277882/1‑88 (MQ=255)
                                                                                                            aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGTCAGTATCTGACCCGAAAGt                    >  2:337252/1‑135 (MQ=255)
                                                                                                            aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGt                    <  5:79491/135‑1 (MQ=255)
                                                                                                            aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTGGGCCCGGCTCGGACCCGAAAgg                    >  3:204198/1‑134 (MQ=255)
                                                                                                            aCTGCTTCTTTGATCGGAGCCAGCTCGGGCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATACGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGt                    <  5:79489/135‑1 (MQ=255)
                                                                                                             cTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTa                   >  3:12471/1‑135 (MQ=255)
                                                                                                                 ttcttTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgat                                                                                                >  5:303659/1‑54 (MQ=255)
                                                                                                                 ttcttTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgat                                                                                                <  6:303659/54‑1 (MQ=255)
                                                                                                                              gCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgat  <  3:287298/135‑1 (MQ=255)
                                                                                                                              gCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgat  <  5:397234/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                      
TTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGAT  >  ACB122/5009566‑5009826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑