Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,083,473 C→T 6.3% A112T (GCC→ACC)  aruG ← Arginine N‑succinyltransferase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,083,4730CT 6.3% 209.4 / 3.3 63A112T (GCC→ACC) aruGArginine N‑succinyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base T (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCGAACAGCTCGGGGAACTCGGCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATGCCCAC  >  ACB122/5083343‑5083599
                                                                                                                                  |                                                                                                                              
ccGAACAGCTCGGGGAACTCGGCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAg                                                                                                                             <  2:228963/134‑1 (MQ=255)
                acgcGGCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGAttt                                                                                                            <  1:212276/132‑1 (MQ=255)
                aCTCGGCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGAttt                                                                                                            <  2:28443/135‑1 (MQ=255)
                     gCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTcgcg                                                                                                        <  5:171073/134‑1 (MQ=255)
                     gCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTcgcg                                                                                                        >  4:190816/1‑134 (MQ=255)
                     gCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTcgcg                                                                                                        >  3:43856/1‑134 (MQ=255)
                     gCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCgg                                                                                                       <  4:46618/135‑1 (MQ=255)
                         tGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCgg                                                                                                       >  8:375401/1‑131 (MQ=255)
                              aGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTc                                                                                              <  2:265912/135‑1 (MQ=255)
                                 aTGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGCAACAGCGTCGGGAATTCGCGGTAGATCTTCAGc                                                                                           >  6:260503/1‑135 (MQ=255)
                                   gcgcgcgcgcGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTcc                                                                                        >  8:228196/1‑136 (MQ=255)
                                   gcgcgcgcgcGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTc                                                                                         >  4:54243/1‑135 (MQ=255)
                                   gcgcgcgcgcGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTc                                                                                         >  5:149480/1‑135 (MQ=255)
                                   gcgcgcgcgcGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTc                                                                                         >  5:156357/1‑135 (MQ=255)
                                   gcgcgcgcgcGACAGCAGGCGGCCAGTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGACCAGCGTCGGGATTTCGCGGTAGATCTTCAGCTc                                                                                         >  5:99025/1‑135 (MQ=255)
                                           gcgACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGTTCGTTGGCCAGGAACAGCGTCGGGAGTTCGCGGTAGATCTTCAGCTCCTGCGAgg                                                                                 >  4:22356/1‑135 (MQ=255)
                                                  cagGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGAc                                                                          <  4:53780/135‑1 (MQ=255)
                                                      cggcCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCAGCGAGGCGCTGACAGTCa                                                                     <  7:174745/136‑1 (MQ=255)
                                                       ggcCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCa                                                                     <  2:62192/135‑1 (MQ=255)
                                                       ggcCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAg                                                                    <  8:140173/136‑1 (MQ=255)
                                                               aGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGc                                                                  <  8:249099/130‑1 (MQ=255)
                                                               aGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGc                                                                  >  7:249099/1‑130 (MQ=255)
                                                                ggCGCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGGCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCGGACAGTCAGGCCGAcc                                                             <  4:327617/134‑1 (MQ=255)
                                                                  cccGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCgg                                                          <  3:206654/135‑1 (MQ=255)
                                                                    cGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAg                                                                                                                       <  7:220485/72‑1 (MQ=255)
                                                                    cGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAg                                                                                                                       >  8:220485/1‑72 (MQ=255)
                                                                      aGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCg                                                                                    >  7:293424/1‑105 (MQ=255)
                                                                      aGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCg                                                                                    <  8:293424/105‑1 (MQ=255)
                                                                         ggTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGGCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGt                                                   <  3:269198/135‑1 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGTCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAg                                              >  2:307870/1‑135 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGTCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAg                                              >  2:313148/1‑135 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCa                                                                     >  8:186565/1‑112 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCa                                                                     <  7:186565/112‑1 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCa                                               <  6:180010/134‑1 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAg                                              >  5:211963/1‑135 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAg                                              >  1:183726/1‑135 (MQ=255)
                                                                              tCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGGAGTTGTACCAg                                              >  4:76791/1‑135 (MQ=255)
                                                                                     gcAGGAACAGCGAGCTCAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCCGGCCGCCCCGGTAGTTGTACCAGGGCTCAc                                       >  2:116498/1‑135 (MQ=255)
                                                                                     gcAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTc                                         <  3:108910/133‑1 (MQ=255)
                                                                                       aGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCAc                                       >  2:212453/1‑133 (MQ=255)
                                                                                          aaCAGCGAGCACAGCTCTGAGTTGCCGGGCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGc                                 <  7:362783/136‑1 (MQ=255)
                                                                                           aCAGCGAGCACAGCTCTGAGGTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGc                                 <  4:274830/135‑1 (MQ=255)
                                                                                                 aGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCCCGCAGGCCGCCgg                           >  5:178605/1‑135 (MQ=255)
                                                                                                 aGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACgg                           >  1:87885/1‑135 (MQ=255)
                                                                                                  gCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGCCCCGCTAGTTGTACCAGGGCTCACGCAGGCCGCCGgc                          >  5:351932/1‑135 (MQ=255)
                                                                                                       gCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCgg                     <  3:80752/135‑1 (MQ=255)
                                                                                                           tGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCCGGGCTCCCGCAGGCCGACGGCCCCGGCgct                 >  1:373116/1‑133 (MQ=255)
                                                                                                           tGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTc                                         <  3:227364/111‑1 (MQ=255)
                                                                                                           tGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTc                                         <  3:231980/111‑1 (MQ=255)
                                                                                                           tGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTc                                         >  4:227364/1‑111 (MQ=255)
                                                                                                           tGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTc                                         >  4:231980/1‑111 (MQ=255)
                                                                                                               ttGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGAGTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCGGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCGCACGCAGGCCGACGGCGCCGGCGATGGCa             <  3:193839/135‑1 (MQ=255)
                                                                                                                tGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGCGGCGCTGACAGTCAGGCCGCCCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACt           >  7:90716/1‑136 (MQ=255)
                                                                                                                tGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGacac            >  2:74461/1‑135 (MQ=255)
                                                                                                                tGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCAc            >  2:25288/1‑135 (MQ=255)
                                                                                                                    ggTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGAt        <  4:57206/135‑1 (MQ=255)
                                                                                                                     gTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTcc                                                                                        >  3:20031/1‑54 (MQ=255)
                                                                                                                     gTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTcc                                                                                        <  4:20031/54‑1 (MQ=255)
                                                                                                                     gTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCActgctg       >  2:199773/1‑135 (MQ=255)
                                                                                                                     gTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATg       >  1:271974/1‑135 (MQ=255)
                                                                                                                     gTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATg       >  1:62667/1‑135 (MQ=255)
                                                                                                                          gTCGTTGGTCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATGCccac  <  1:307870/135‑1 (MQ=255)
                                                                                                                          gTCGTTGGTCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATGCccac  <  1:313148/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                              
CCGAACAGCTCGGGGAACTCGGCAATGAACAGCATGCGCGCGCGCGACAGCAGGCGGCCATTGAGGCCCGAGCGGTAGTCGCTGCGCAGGAACAGCGAGCACAGCTCTGAGTTGCCGGTCAGGTCGTTGGCCAGGAACAGCGTCGGGATTTCGCGGTAGATCTTCAGCTCCTGCGAGGCGCTGACAGTCAGGCCGACCCGGTAGTTGTACCAGGGCTCACGCAGGCCGACGGCGCCGGCGATGGCACTGATGCCCAC  >  ACB122/5083343‑5083599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑