Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,086,965 T→C 6.1% K209E (AAG→GAG)  cdhR_7 ← HTH‑type transcriptional regulator CdhR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,086,9650TC 6.1% 210.5 / 3.8 65K209E (AAG→GAG) cdhR_7HTH‑type transcriptional regulator CdhR
Reads supporting (aligned to +/- strand):  ref base T (36/25);  new base C (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GGGCACGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGCCAG  >  ACB122/5086831‑5087091
                                                                                                                                      |                                                                                                                              
gggCACGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTg                                                                                                                               >  8:56279/1‑136 (MQ=255)
 ggCACGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTg                                                                                                                               <  1:85087/135‑1 (MQ=255)
  gctcgcgTTTAAGGTACTGCTTGAAGAACCGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGGGGTCAGCGGCGCTTCGTTGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTgg                                                                                                                              <  2:379272/132‑1 (MQ=255)
  gCACGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTgg                                                                                                                              >  6:157954/1‑135 (MQ=255)
    aCGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTgggg                                                                                                                            >  1:251496/1‑135 (MQ=255)
     cgcgGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGt                                                                                                                           >  5:60075/1‑135 (MQ=255)
     cgcgGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGt                                                                                                                           >  6:259286/1‑135 (MQ=255)
          ttCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACt                                                                                                                       >  1:379272/1‑134 (MQ=255)
                     cTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCg                                                                                                           >  6:240891/1‑135 (MQ=255)
                     cTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCg                                                                                                           >  6:377349/1‑135 (MQ=255)
                       tGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGt                                                                                                         >  1:137480/1‑135 (MQ=255)
                       tGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGt                                                                                                         >  3:137008/1‑135 (MQ=255)
                       tGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGt                                                                                                         >  1:233463/1‑135 (MQ=255)
                       tGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGt                                                                                                         >  2:152280/1‑135 (MQ=255)
                         aaGATACGCTCCAACTGCCGCCGCGACACACACACATGCTGGGCGAGTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCCGCACCACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGGTc                                                                                                       >  4:305689/1‑135 (MQ=255)
                          aGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCt                                                                                                      <  3:194906/135‑1 (MQ=255)
                          aGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGAGTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTg                                                                                                     <  8:335626/136‑1 (MQ=255)
                           gATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTg                                                                                                     <  2:137480/135‑1 (MQ=255)
                           gATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGGGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTg                                                                                                     <  5:157954/135‑1 (MQ=255)
                            aTACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTg                                                                                                                          <  5:42527/113‑1 (MQ=255)
                            aTACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTg                                                                                                                          >  6:42527/1‑113 (MQ=255)
                            aTACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGCGCCCAGGCGGTTCt                                                                                                      >  6:125261/1‑133 (MQ=255)
                                gCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCg                                                                                                <  3:22464/135‑1 (MQ=255)
                                  tCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGa                                                                                              >  4:139869/1‑135 (MQ=255)
                                  tCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGCGTGACTCGAGCCCAGGCGGTTCTGCAGCGGa                                                                                              >  4:100866/1‑135 (MQ=255)
                                  tCCAACTGCCGACGCGACACACACACATGATGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGATTGGGGTGACTCGAGCCCAGGCGGTTCTTCAGAGGa                                                                                              >  2:129489/1‑135 (MQ=255)
                                    cAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATa                                                                                           <  7:235850/136‑1 (MQ=255)
                                             aCGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGgcg                                                                                   >  5:281359/1‑135 (MQ=255)
                                                            aTGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTcgc                                                                     >  3:120892/1‑134 (MQ=255)
                                                            aTGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   <  8:50878/136‑1 (MQ=255)
                                                            aTGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   <  7:56279/136‑1 (MQ=255)
                                                              gCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGa                                                                  <  5:259286/135‑1 (MQ=255)
                                                                tGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACCCGGCCTGAGTCAGCTTGGGGGGACTCGAGCCCAGGCGGTTCTGCAGCGGAGTACGCTGGCGCTCGCCCCCCTCGCGGATgc                                                               >  8:289982/1‑136 (MQ=255)
                                                                   gCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATgcgc                                                             <  5:377349/135‑1 (MQ=255)
                                                                   gCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATgcgc                                                             >  3:41319/1‑135 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCt                                                                                                      <  7:95975/92‑1 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCt                                                                                                      >  8:95975/1‑92 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGct                                                            >  5:148855/1‑134 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGct                                                            >  3:143889/1‑134 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGct                                                            >  5:100929/1‑134 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTCGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   <  8:327492/127‑1 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTCGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   <  8:321909/127‑1 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTCGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   >  7:327492/1‑127 (MQ=255)
                                                                     gATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTCGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCgg                                                                   >  7:321909/1‑127 (MQ=255)
                                                                       tttCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTac                                                        >  8:279849/1‑136 (MQ=255)
                                                                         tcgtcgGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTacc                                                       >  4:213964/1‑135 (MQ=255)
                                                                          cgtcgGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGGGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTaccac                                                     <  7:236371/136‑1 (MQ=255)
                                                                               gtggtCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATgcgc                                                             >  8:124232/1‑123 (MQ=255)
                                                                               gtggtCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATgcgc                                                             <  7:124232/123‑1 (MQ=255)
                                                                                      gCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCg                                          <  4:137008/135‑1 (MQ=255)
                                                                                      gCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGGGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCg                                          <  5:144059/135‑1 (MQ=255)
                                                                                             ttCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGc                                   >  6:296051/1‑135 (MQ=255)
                                                                                                       gCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGc                         >  4:112894/1‑135 (MQ=255)
                                                                                                                 gccacacGCCCTGAGGCAGGTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGGTGAATACGCTGGCGCTCGCCACCCTCGCGGATGGGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGt               <  1:158804/132‑1 (MQ=255)
                                                                                                                  cAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGTCCCGGCCAGCTCCGCCCCGTGa             <  8:40243/136‑1 (MQ=255)
                                                                                                                   aaCACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGa             <  2:30114/135‑1 (MQ=255)
                                                                                                                        ggCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCAGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCCGATCTTCCGACACTGCCCCGGCCCGCTCCGCCCCGTGATcgcgc       >  8:186919/1‑136 (MQ=255)
                                                                                                                           cTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGcc    >  7:309723/1‑136 (MQ=255)
                                                                                                                             gAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGcc                                  <  7:136265/104‑1 (MQ=255)
                                                                                                                             gAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGcc                                  >  8:136265/1‑104 (MQ=255)
                                                                                                                             gAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGCCAg  >  8:255300/1‑136 (MQ=255)
                                                                                                                              aGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGCCa   <  8:309723/134‑1 (MQ=255)
                                                                                                                               gTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTac                                                        <  3:3452/80‑1 (MQ=255)
                                                                                                                               gTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTac                                                        >  4:3452/1‑80 (MQ=255)
                                                                                                                               gTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGCCa   <  5:158373/133‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
GGGCACGCGGTTCAGGTACTGCTTGAAGATACGCTCCAACTGCCGACGCGACACACACACATGCTGGGCGATTTCGTCGGTGGTCAGCGGCTCTTCGATGTTGGCTTCCATCAGCAACACGGCCTGAGTCAGCTTGGGGTGACTCGAGCCCAGGCGGTTCTGCAGCGGAATACGCTGGCGCTCGCCACCCTCGCGGATGCGCTCTACCACCAGCTCTTCCGACACTGCCCCGGCCAGCTCCGCCCCGTGATCGCGCGCCAG  >  ACB122/5086831‑5087091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑