Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,359,751 A→G 7.5% I258T (ATC→ACC)  ftsH ← ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,359,7510AG 7.5% 149.5 / 2.1 53I258T (ATC→ACC) ftsHATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base A (23/26);  new base G (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTT  >  ACB122/5359624‑5359886
                                                                                                                               |                                                                                                                                       
ggcgATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgtc                                                                                                                                  <  5:254515/135‑1 (MQ=255)
  cgatgacgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGa                                                                                                                                <  1:298779/135‑1 (MQ=255)
  cgatgacgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGa                                                                                                                                <  6:152893/135‑1 (MQ=255)
   gatgacgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGCCCAACGGCGTCGATTtcgtcgg                                                                                                                                >  2:298779/1‑133 (MQ=255)
   gatgacgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGCCCAACGGCGTCGATTTCGTCGAt                                                                                                                               >  1:248043/1‑135 (MQ=255)
        cgatgaTGCCATCATTCATCTCGAAGCCGGCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCTCGCTCGTGGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAg                                                                                                                           <  2:374548/134‑1 (MQ=255)
            gatgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatgat                                                                                                                      <  3:16927/135‑1 (MQ=255)
            gatgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatgat                                                                                                                      <  1:219710/135‑1 (MQ=255)
            gatgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatgat                                                                                                                      <  3:217670/135‑1 (MQ=255)
             atgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatgat                                                                                                                      <  3:13742/134‑1 (MQ=255)
                  atcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGCCCAACGGCGTCCATTTAGTCGATGAAGATTATGCACggg                                                                                                               >  8:173305/1‑136 (MQ=255)
                   tcattcatCTGGAAGCCGTCCATCTCCACCAGCAAGTGGTTGAGGGGTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              <  8:147873/136‑1 (MQ=255)
                   tcattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              <  7:198015/136‑1 (MQ=255)
                    cattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              <  4:153346/135‑1 (MQ=255)
                    cattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              <  1:295044/135‑1 (MQ=255)
                              gAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGcttctt                                                                                                    <  8:335186/135‑1 (MQ=255)
                                   cGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCGCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGGCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCt                                                                                               <  1:79359/135‑1 (MQ=255)
                                        aTCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTGGGCGTTGAAGATGATGCAACGGGCGTTCTTCTTGGCCGGGTCg                                                                                          >  1:199498/1‑135 (MQ=255)
                                        aTCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCg                                                                                          >  7:279074/1‑135 (MQ=255)
                                                  gCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGCCCAACGGCGTCGAGTTCGTCGATGCAACTGATTCCCGGGGAGTGGGGCTTTGGCTGCGCGAACCTggggc                                                                                >  6:250574/1‑131 (MQ=255)
                                                  gCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTcgc                                                                                <  3:131756/135‑1 (MQ=255)
                                                  gCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGGCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTcgc                                                                                <  3:385015/135‑1 (MQ=255)
                                                   cAACTGGTTGAGGGTTTGCTCACGCTAGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCGTGGCCTGCTCGAACATGTcgcg                                                                               >  1:217121/1‑135 (MQ=255)
                                                    aagtGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCTGCGCCACGATGGCGACCAACGGCGTCGATTTCGTGGATGAAGATGATGCTCGGGGCGTGCTTCTTGGCCTGCTCGAACTTGTcgc                                                                                <  5:367594/130‑1 (MQ=255)
                                                      cTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGccca                                                                           >  7:59462/1‑136 (MQ=255)
                                                                gTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCAc                                                                 >  7:119830/1‑136 (MQ=255)
                                                                 cttGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCAc                                                                 <  4:45350/134‑1 (MQ=255)
                                                                    gCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTcgcg                                                                               <  2:82940/118‑1 (MQ=255)
                                                                    gCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTcgcg                                                                               >  1:82940/1‑118 (MQ=255)
                                                                    gCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCCCCCGGCTGGCCCCgc                                                              >  2:362567/1‑134 (MQ=255)
                                                                      tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGCTGCACGGGGCGTGCTTCTTGGCCTGGTCGAACATGTCGCGCACACGGCGGGCACCgccc                                                            >  5:220369/1‑132 (MQ=255)
                                                                      tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGaca                                                            <  3:159925/135‑1 (MQ=255)
                                                                      tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGAGTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCCCCGaca                                                            >  5:140423/1‑135 (MQ=255)
                                                                       cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGGTTTCGTCGATGAAGATGATGc                                                                                                                    <  1:179443/78‑1 (MQ=255)
                                                                       cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGGTTTCGTCGATGAAGATGATGc                                                                                                                    <  1:184897/78‑1 (MQ=255)
                                                                       cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGGTTTCGTCGATGAAGATGATGc                                                                                                                    >  2:184897/1‑78 (MQ=255)
                                                                       cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGGTTTCGTCGATGAAGATGATGc                                                                                                                    >  2:179443/1‑78 (MQ=255)
                                                                       cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATGTCGGCGATGAAGATGATGCACGGGGCGGGCTGCTTGGCCTGCGCGGACCTGTCGCGCCCCCGGCTGGCCCCgccccc                                                          >  7:344413/1‑131 (MQ=255)
                                                                             cgtcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCGGGCACCGACCCCCAcca                                                     >  4:34630/1‑133 (MQ=255)
                                                                                     ccgccgCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTcc                                             <  5:149305/135‑1 (MQ=255)
                                                                                       gccgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACCCGGCTGGCCCCGACACCCACGCACATTTCCa                                            >  2:64831/1‑134 (MQ=255)
                                                                                       gccgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGGGCGTGCTGGTTTGCCTGCTCGAAAATGACTCGCGAACGGCTCGGCCCGCCCCCCCCGATCATATCCACg                                          >  8:341815/1‑136 (MQ=255)
                                                                                         cgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGaa                                        <  7:173305/136‑1 (MQ=255)
                                                                                           cccATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTAGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAg                                       >  4:175373/1‑135 (MQ=255)
                                                                                                  cGGCGCCACGATGGCGCCCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGCACATGTCGCGCCCCCGGCTGGCCCCGCCCCCCCCGCACCTTTCCACGAAGTCCGAAc                                >  1:328137/1‑135 (MQ=255)
                                                                                                       ccACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCAGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCCCGAACATTTCCACGAAGTCCGCACCGGaa                           >  1:70943/1‑135 (MQ=255)
                                                                                                        cACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              <  7:315159/51‑1 (MQ=255)
                                                                                                        cACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACgggg                                                                                                              >  8:315159/1‑51 (MQ=255)
                                                                                                         aCGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATg                        <  7:117265/136‑1 (MQ=255)
                                                                                                             tGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCTCGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATtgtg                     <  1:11194/135‑1 (MQ=255)
                                                                                                                           gTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACttt       <  2:13586/135‑1 (MQ=255)
                                                                                                                            tCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGCCACCCACGAACATTTCCACGAAGTACGAACCGGAAATGGTGCAGAACGGGACTttcg     >  8:175538/1‑136 (MQ=255)
                                                                                                                            tCGATTTCGTCGATGAAGATGAATCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCAACGAACCCCACGAACATTTCAACGAAGTACGAACCGCAAAAGGTGAACAACGGTACAttcg     >  8:175542/1‑136 (MQ=255)
                                                                                                                               aTTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGCCACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTttcgct   >  4:190685/1‑135 (MQ=255)
                                                                                                                               aTTTCGGCGATGAAGATGATGCACGGGGCGTGCGTCTTGGCCTGCTCGAACATGTAGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAACTCCGAACCGGAAATGGTGAAGAACGGTACAttcgctt  >  8:320794/1‑136 (MQ=255)
                                                                                                                               |                                                                                                                                       
GGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTT  >  ACB122/5359624‑5359886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑