Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,409,654 A→G 8.2% L226P (CTG→CCG)  gudD ← Glucarate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,409,6540AG 8.2% 142.6 / 4.7 50L226P (CTG→CCG) gudDGlucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (26/18);  new base G (2/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

CGTTCTCGGCGCCGCAGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGCACCACTGCTTCCGGGGTCAGGGCCTTTTCGTG  >  ACB122/5409520‑5409787
                                                                                                                                      |                                                                                                                                     
cGTTCTCGGCGCCGCTGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGAGGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCa                                                                                                                                       <  2:339992/135‑1 (MQ=255)
cGTTCTCGGCGCCGCAGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCa                                                                                                                                       <  4:177090/135‑1 (MQ=255)
        gcgcCGCAGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTc                                                                                                                               <  6:104948/135‑1 (MQ=255)
              cAGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGcc                                                                                                                         <  5:155498/135‑1 (MQ=255)
               aGGGGTCTTCGGCGTAGGCCACCACATAGAGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATCCGCGCTTCGGGGACGCGTTCGGCCAGCGCTGTCACGGccg                                                                                                                        >  1:376794/1‑134 (MQ=255)
                      ttCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGTGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATctct                                                                                                                >  8:291199/1‑136 (MQ=255)
                                 ccAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTcaccac                                                                                                      >  3:288005/1‑135 (MQ=255)
                                        aTTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACAACGCAGCAc                                                                                               >  5:357689/1‑135 (MQ=255)
                                             gCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCAtct                                                                                                                  >  8:75659/1‑111 (MQ=255)
                                             gCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCAtct                                                                                                                  <  7:75659/111‑1 (MQ=255)
                                             gCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCAcgccgc                                                                                          >  1:265266/1‑135 (MQ=255)
                                             gCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCAcgccgc                                                                                          >  3:286392/1‑135 (MQ=255)
                                                    aCGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAg                                                                                   >  3:186759/1‑135 (MQ=255)
                                                    aCGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAg                                                                                   >  5:54990/1‑135 (MQ=255)
                                                    aCGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGc                                                                                  >  7:48367/1‑136 (MQ=255)
                                                       gCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTg                                                                               <  8:313501/136‑1 (MQ=255)
                                                         acaGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCtt                                                                                >  5:391196/1‑133 (MQ=255)
                                                                gATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGc                                                                        >  1:41828/1‑134 (MQ=255)
                                                                gATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGc                                                                        >  2:81097/1‑134 (MQ=255)
                                                                   ggCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTgca                                                                    >  4:374792/1‑133 (MQ=255)
                                                                     cctcctTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCa                                                                                                                     <  4:12196/84‑1 (MQ=255)
                                                                     cctcctTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCa                                                                                                                     >  3:12196/1‑84 (MQ=255)
                                                                     cctcctTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAg                                                                   >  3:29230/1‑134 (MQ=255)
                                                                       tcctTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCa                                                                <  6:54990/135‑1 (MQ=255)
                                                                          ttCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCCGCGCTGTCCCGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCCGGCGCTTGCAGTCGCTGACGCCagag                                                             >  1:202122/1‑135 (MQ=255)
                                                                          ttCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAg                                                             >  2:211986/1‑135 (MQ=255)
                                                                                  ccACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTg                                                                               >  5:27481/1‑109 (MQ=255)
                                                                                  ccACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTg                                                                               <  6:27481/109‑1 (MQ=255)
                                                                                    aCGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCGGCGCTg                                                                                                                                 >  8:213408/1‑57 (MQ=255)
                                                                                    aCGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCGGCGCTg                                                                                                                                 <  7:219058/57‑1 (MQ=255)
                                                                                    aCGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCGGCGCTg                                                                                                                                 >  8:219058/1‑57 (MQ=255)
                                                                                    aCGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCGGCGCTg                                                                                                                                 <  7:213408/57‑1 (MQ=255)
                                                                                          cATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACgcagc                                                                                                 >  5:377622/1‑83 (MQ=255)
                                                                                          cATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACgcagc                                                                                                 <  6:377622/83‑1 (MQ=255)
                                                                                               gggTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGc                                         <  5:99678/134‑1 (MQ=255)
                                                                                                ggTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGcc                                        <  4:78982/134‑1 (MQ=255)
                                                                                                     aGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTAGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGc                                  >  6:150002/1‑135 (MQ=255)
                                                                                                      gCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGca                                 <  4:29230/135‑1 (MQ=255)
                                                                                                       cGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATGTGTTCTACACCACGCAGCACGCCGCCCTAGAGCATGATATCGCTGAAGCAATAGCGCGCCATGGCGGCCTACGCCACCCGccc                                >  2:378933/1‑133 (MQ=255)
                                                                                                       cGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCCCGCCGCCCTGGAGAGTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGTCAAGCCGcac                                >  2:357565/1‑135 (MQ=255)
                                                                                                       cGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGccc                                >  3:163746/1‑133 (MQ=255)
                                                                                                       cGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGcac                                <  4:288005/135‑1 (MQ=255)
                                                                                                       cGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGcac                                <  4:286392/135‑1 (MQ=255)
                                                                                                        gggATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGcacca                              <  7:359871/134‑1 (MQ=255)
                                                                                                            tGCGCGCTTCGGGGAAGCGTTCGGCCCGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGcaccacgg                           >  6:198210/1‑133 (MQ=255)
                                                                                                                   ttCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGCACCACTGCTTCCgg                    <  4:186759/135‑1 (MQ=255)
                                                                                                                    tCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGCACCACTGCTTCCggg                   >  5:113261/1‑135 (MQ=255)
                                                                                                                        ggAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCt                                                                                 <  6:153068/69‑1 (MQ=255)
                                                                                                                        ggAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCt                                                                                 >  5:153068/1‑69 (MQ=255)
                                                                                                                                  ggCCAGCGCTGTCACGGCCTCCATCTCTTCTTcaccac                                                                                                      <  7:262358/38‑1 (MQ=255)
                                                                                                                                  ggCCAGCGCTGTCACGGCCTCCATCTCTTCTTcaccac                                                                                                      >  8:262358/1‑38 (MQ=255)
                                                                                                                                    ccAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGCACCACTGCTTCCGGGGTCAGGGCCTTTTCGTg  <  7:291199/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CGTTCTCGGCGCCGCAGGGGTCTTCGGCGTAGGCCAGCACATTGTGCTTGTCACGGCACAGGGCGATGGCCTCCTTCAGCGACCACGCGCCATTGGGGTCGAGCGTGATGCGCGCTTCGGGGAAGCGTTCGGCCAGCGCTGTCACGGCCTCCATCTCTTCTTCACCACGCAGCACGCCGCCCTTGAGCTTGAAGTCGCTGAAGCCATAGCGCGCCTTGGCGGCCTCGGCCAGCCGCACCACTGCTTCCGGGGTCAGGGCCTTTTCGTG  >  ACB122/5409520‑5409787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑