Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	6,020,340	0	A	C	46.7%	26.6 / 18.5	64	L58R (CTG→CGG)	ycaD_4	putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base A (7/25);  new base C (20/8);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 2.11e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTG  >  ACB122/6020206‑6020471                                                                                                                                       |                                                                                                                                     cGATCATCCGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                      <  7:73013/135‑1 (MQ=255)      atcaGCCGCAGCAGCAGCCTTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGcag                                                                                                                                 >  6:136031/1‑133 (MQ=255)      atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACATGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGAGGCCCGGCTg                                                                                                                                 >  2:114730/1‑135 (MQ=255)       tcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                                 <  7:143252/134‑1 (MQ=255)          gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGg‑cg                                                                                                                             <  4:12577/134‑1 (MQ=255)          gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCCCGCTGg‑cgc                                                                                                                            >  4:395423/1‑135 (MQ=255)               cgccgcagcCAAACCGGGTACCTAGGCAGCAGCGCCAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACTGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCGGG‑CGCCGGcc                                                                                                                       <  5:527537/131‑1 (MQ=255)               agcagcagcCATAACGGGTAACTAGGCTGCAGCGCGAGCAGGCCAATCGCCAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGcc                                                                                                                       <  3:413012/135‑1 (MQ=255)                       ccATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGCCCGGCTGG‑CGCCCGCCCGTACGcc                                                                                                               >  6:314779/1‑135 (MQ=255)                       ccATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCGGG‑CGCCGGCCACGACGcc                                                                                                               >  2:470439/1‑135 (MQ=255)                         aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGGCACGCTTG‑CGCCCGCCCCGACGcccc                                                                                                             >  6:423010/1‑134 (MQ=255)                              gggTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCACCCGGG‑CGCCCGCCCGGACGCCCCAcccc                                                                                                        >  3:39842/1‑135 (MQ=255)                                    ctcggCAGCCGCGCGAGCCGGCCAATCCCCCGCGCCCCGCCCCCCCGGCACCGGCGCTTCGCCGACGGTACGCCAACCCAACCTGCCCGGGGGCTGGCCAGGCTGG‑CGCGGCCCATTACGCCCAACGCCGGCAt                                                                                                   <  4:458102/131‑1 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGTCTGG‑CGCCGGCCAGTACGCCCAACGCCCGCCtt                                                                                                  >  8:177118/1‑135 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGG‑CGCCGGCCCCTACGCCCAACGCCGGCAtt                                                                                                  >  4:82963/1‑135 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCGGG‑CCCCCGCCCCGACGCCCCACCCCCGccat                                                                                                  >  1:318547/1‑132 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCTGG‑CGCCGGCCAGTACGCCCAACGCCCGCaat                                                                                                  >  7:414914/1‑133 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCCCCCGGCCAGACCCCCCACCCCCGGCaa                                                                                                   >  3:289135/1‑134 (MQ=255)                                    cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGCG‑CGCCCGCCCGCACCCCCCAccccccccat                                                                                                  >  6:26572/1‑129 (MQ=255)                                         ccgccgcgcgcgcCGGCCAAGCCCCCGCGCCCCGCCCCCCCGCCACAGGGGCACCGCCGAAGGTCCGCCACCCCACCCTGCCTGCCGGCTGGCCCGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTgccgc                                                                                             <  8:513993/130‑1 (MQ=255)                                         ccccagcGCGAGCCGGCCAATCGCCAGCGCCCCGCCCCACAGGCACAGGCGCAGCGCCGAAGGTACGCCAACCCACCCTGCCCGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTgccgc                                                                                             <  2:447800/132‑1 (MQ=255)                                                  gAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGG‑CGGCGGCCCCTACGCCCCACGCCCGCCGTGCCGCCCTCCCGcc                                                                                    >  1:355103/1‑135 (MQ=255)                                                    gccggCCAAGCGCCCGCGCCCCGCCCCCCCGGCACCGGCGCATCGCCGACGGTACGCCACCCCAACCGGCCCGGCGGCTGGCCAGGCGGG‑CGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGa                                                                                  <  8:251570/132‑1 (MQ=255)                                                    gCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGG‑CGCCCGCCCGGCCGCCCCACCCCGGCCTTGGCGCCATCAcccc                                                                                    >  7:128497/1‑133 (MQ=255)                                                          cAATCGACAGCGCCCCGCCCCACCGGCACAGGCGCATCGCCGACGGTACGCCAACCCAACCTGCCCGGCGGCTGGCCCGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGa                                                                            <  4:405992/135‑1 (MQ=255)                                                           aaTCGACATCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                      >  3:121463/1‑77 (MQ=255)                                                           aaTCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                      <  4:121463/77‑1 (MQ=255)                                                           aaTCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGG‑GGCCGGCCCGTACGCCCCACCCCCGCCTTGCCCCCCGCACGCCGCAGGCgga                                                                           >  6:112607/1‑133 (MQ=255)                                                                cccccgccccgcccCACCGGCACTGGCGCATCGCCGAAGGTACGCCAACCCACCCTGCCCGGGGGCTGGCCAGGGGGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTg                                                                       <  8:122764/130‑1 (MQ=255)                                                                     gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCGGG‑CGCCGGGCAGTACGCCCAACGCCCGCCTTGCCGCCATCACGCCCATGGCGAAGCTGCCGTAg                                                                 >  2:327995/1‑135 (MQ=255)                                                                     gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCCGGCTGG‑CGCCGGCCCGTACGCCCCACCCCCGGCGTGCCGCCCACACGCCGCTGGCGCCGCTGCCGGAg                                                                 >  6:163891/1‑135 (MQ=255)                                                                       cccgccccACCGGCACCGGCGCATCGCCGACGGTCCGCCACCCCAACCTGCCCGGCGGCGGGCCAGGCTGG‑CGCCGGCCTGTCCGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcc                                                               <  3:528440/135‑1 (MQ=255)                                                                         cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGGGGGCCGGCGGG‑CGCCGGCCCGGTCGCCCCCCGCCGGCCGTGCCGCCAGCGCCCCGCAGGCGCCGCTGCCGGAGcccc                                                             >  1:391801/1‑135 (MQ=255)                                                                            cccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGGCCCGCGGG‑CGCCGGCCCCTACGCCCCACGCCGCCCATGCCGCCCGCACCCCCAAGGCGCAGCTGCCCTAGccccccg                                                          >  2:436553/1‑132 (MQ=255)                                                                            cccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGG‑CGCCCGCCACTACCCCCAACCCCCGCAATTCCGCCAACACGCCCATTGCGAAGCTGCCGAAGCCCCAgg                                                          >  8:522897/1‑135 (MQ=255)                                                                            cccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGG‑CGCCGGGCCGGACGCCCCCCCCCCGCCGTGCCGCCCGCCCCCCCCTGGGGGCGCGGCCGTAGccccccg                                                          >  1:177698/1‑132 (MQ=255)                                                                            cccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGG‑CGCCCGCCCGTCCGCCCCACGCCCGCATTGCCGCCCTCACCCCGCTGGCGAAGCTGCCGTAGccccccg                                                          >  7:443768/1‑132 (MQ=255)                                                                                 cggcacaggcGCATCGCCGAAGGAACGCCAACCCAACCTGCCCGGCGGCTGGCCAGGCGGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTcc                                                     <  6:247690/134‑1 (MQ=255)                                                                                    aacaggcGCAGCGCCGACGGAACCCCACCCCACCCTGCCCGGCGGCTGGCCAGGCGGG‑CGCCGGCCAGTACGCCAAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAgg                                                  <  7:86393/134‑1 (MQ=255)                                                                                       cggcGCAGCGCCGACGGTACCCCAACCCACCCTGCCCGGGGGCTGGCGAGGCTGG‑GGCCGGCCTGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                               <  3:318807/134‑1 (MQ=255)                                                                                       cgccgaaTCGCCGATGTTACCCCACCCCAACCTCCCCGGCGGCGGGCCAGGGGGG‑CGCGGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                               <  5:389409/129‑1 (MQ=255)                                                                                       aggcGCATCCCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGGTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                               <  8:175013/135‑1 (MQ=255)                                                                                            cATCGCCGACGGTACGCCAACCCAACCTGCCCGGCGGCTGGCCAGGCTGG‑CGCCGCCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGa                                          <  8:260720/135‑1 (MQ=255)                                                                                              gcGCCTAAGGTACGCCAACCCACCCTCCCAGGCGGCTGGCCAGGCGGG‑CGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGaca                                        <  6:389520/134‑1 (MQ=255)                                                                                                 ccGAAGGTACGCCAACCCAACCTGCCAGGCGGCTTGCCACGCTGG‑GCCCGGCCAGTACGCCCAACGCCCGCCATGCCGCCCTCACGCCCCAGGCGAAGCTGCCGGAGCCCCCGGCCCCCACGCGCAGGGACACCa                                     >  7:452839/1‑135 (MQ=255)                                                                                                  cGAAGGTACGCCAACCCAACCTGCCCGGCGGCGGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                    <  4:35132/135‑1 (MQ=255)                                                                                                        taggCCAACCCAACCCGCCAGGCGGCTGGCCTGGCTGG‑CGCCGGCGATTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                              <  1:5598/132‑1 (MQ=255)                                                                                                        tACGCCAACCCACCCTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                              <  6:104298/135‑1 (MQ=255)                                                                                                        gaCGCCAACCCAACCTGCCAGGGGGCTGGCCAGGCGGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                              <  8:434749/134‑1 (MQ=255)                                                                                                        aaCGCCAACCCACCCGGCCAGGCGGCGGGCCAGGCGGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                              <  1:321729/134‑1 (MQ=255)                                                                                                             caacccaaccTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGGACGCCCCACGCCGGCATTGCCGCCCTCACCCCGAAGGCGCAGCTGCCGTCGCCCCCCGCCGCCCCGCGCCGGGCCACCCGCGGCATGCTcc                         >  7:66649/1‑134 (MQ=255)                                                                                                              aacccaaccTGCCAGGCGGCTGGCCCGGCTGG‑CGCCGGCCAGGACGCCCCACGCCGGCATTTCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCACCCGGCGCAAGGACACCCCCCGCAAGCTCAc                        >  8:389770/1‑135 (MQ=255)                                                                                                              aacccaaccTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCAc                        <  2:43433/135‑1 (MQ=255)                                                                                                                   aaccTGCCAGGCGGCTGGCCCGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCCTCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGGCACCCGCGGCATGCTCACGccca                   >  1:81588/1‑133 (MQ=255)                                                                                                                    cccggCCGGGCGGCGGGCCTGGGGGG‑GGCGGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  5:129363/131‑1 (MQ=255)                                                                                                                    cccTGCCAGGGGGCGGGCCCGGCGGG‑CGCGGCCAAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  5:423010/134‑1 (MQ=255)                                                                                                                    cccTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  4:259618/134‑1 (MQ=255)                                                                                                                    cccTGCCAGGCGGCTGGCACGGCTGG‑CGCCGGCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  5:129657/134‑1 (MQ=255)                                                                                                                    cccTCCCAGGGGGCGGGCCCGGGGGG‑CGCCGCCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  1:436553/134‑1 (MQ=255)                                                                                                                    accTGCCAGGCGGCTGGCCCGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  3:473363/135‑1 (MQ=255)                                                                                                                    accTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  4:222404/135‑1 (MQ=255)                                                                                                                    accTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCAAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                  <  4:112123/135‑1 (MQ=255)                                                                                                                         cccgggggcTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa             <  1:327995/129‑1 (MQ=255)                                                                                                                         cccggcggcTGGCCTGGGTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa             <  7:389770/132‑1 (MQ=255)                                                                                                                                gcgggCCCGGGTGG‑CGCCGGCCTTGACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACAACAGCGGCATGCTCACGCCAAGCGCCAGCCCGac      <  1:359603/132‑1 (MQ=255)                                                                                                                                gcTGGCCCGGCTGG‑CGCCGGCCTGAACCCCCCACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGac      <  5:475529/135‑1 (MQ=255)                                                                                                                                    gCCTGGCTGG‑CGCGGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTg  <  3:395423/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGG‑CGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTG  >  ACB122/6020206‑6020471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑